Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase.
The enzyme, characterized from the bacterium Streptomyces hygroscopicus, participates in the pathway for bialaphos biosynthesis. It requires a divalent metal ion and can also act on oxaloacetate.
Shimotohno KW, Seto H, Otake N, Imai S, Murakami T
Title
Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293.
Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase.
Enzymatic reactions [BR:br08201]
2. Transferase reactions
2.3 Acyltransferases
2.3.3 Acyl groups converted into alkyl groups on transfer
2.3.3.18
R08870 3-(Hydrohydroxyphosphoryl)pyruvate + Acetyl-CoA + H2O <=> 2-Phosphinomethylmalate + CoA