KEGG   ORTHOLOGY: K12910
Entry
K12910            Tight     KO                                     
Symbol
pmmS
Name
2-phosphinomethylmalate synthase [EC:2.3.3.18]
Pathway
map00440  Phosphonate and phosphinate metabolism
map01110  Biosynthesis of secondary metabolites
Reaction
R08870  acetyl-CoA:phosphinopyruvate C-acetyltransferase (thioester-hydrolysing, carboxymethyl forming)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09106 Metabolism of other amino acids
   00440 Phosphonate and phosphinate metabolism
    K12910  pmmS; 2-phosphinomethylmalate synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.3  Acyltransferases
   2.3.3  Acyl groups converted into alkyl groups on transfer
    2.3.3.18  2-phosphinomethylmalate synthase
     K12910  pmmS; 2-phosphinomethylmalate synthase
Genes
AG: AAU00086(pms) BAA90528(pmmS)
Reference
PMID:19489722
  Authors
Metcalf WW, van der Donk WA
  Title
Biosynthesis of phosphonic and phosphinic acid natural products.
  Journal
Annu Rev Biochem 78:65-94 (2009)
DOI:10.1146/annurev.biochem.78.091707.100215
Reference
PMID:1368511
  Authors
Shimotohno KW, Imai S, Murakami T, Seto H
  Title
Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase.
  Journal
Agric Biol Chem 54:463-70 (1990)
DOI:10.1271/bbb1961.54.463
  Sequence
[ag:BAA90528]
LinkDB

KEGG   ENZYME: 2.3.3.18
Entry
EC 2.3.3.18                 Enzyme                                 
Name
2-phosphinomethylmalate synthase;
pmmS (gene name)
Class
Transferases;
Acyltransferases;
Acyl groups converted into alkyl groups on transfer
Sysname
acetyl-CoA:phosphinopyruvate C-acetyltransferase (thioester-hydrolysing, phosphinomethylmalate-forming)
Reaction(IUBMB)
acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate = phosphinomethylmalate + CoA [RN:R08870]
Reaction(KEGG)
R08870
Substrate
acetyl-CoA [CPD:C00024];
H2O [CPD:C00001];
3-(hydroxyphosphinoyl)pyruvate
Product
phosphinomethylmalate;
CoA [CPD:C00010]
Comment
The enzyme, characterized from the bacterium Streptomyces hygroscopicus, participates in the pathway for bialaphos biosynthesis. It requires a divalent metal ion and can also act on oxaloacetate.
History
EC 2.3.3.18 created 2017
Pathway
ec00440  Phosphonate and phosphinate metabolism
ec01110  Biosynthesis of secondary metabolites
Orthology
K12910  2-phosphinomethylmalate synthase
Reference
1  [PMID:3170341]
  Authors
Shimotohno KW, Seto H, Otake N, Imai S, Murakami T
  Title
Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293.
  Journal
J Antibiot (Tokyo) 41:1057-65 (1988)
DOI:10.7164/antibiotics.41.1057
Reference
2  [PMID:1368511]
  Authors
Shimotohno KW, Imai S, Murakami T, Seto H
  Title
Purification and characterization of citrate synthase from Streptomyces hygroscopicus SF-1293 and comparison of its properties with those of 2-phosphinomethylmalic acid synthase.
  Journal
Agric Biol Chem 54:463-70 (1990)
DOI:10.1271/bbb1961.54.463
  Sequence
[ag:BAA90528]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.3.18
IUBMB Enzyme Nomenclature: 2.3.3.18
ExPASy - ENZYME nomenclature database: 2.3.3.18
BRENDA, the Enzyme Database: 2.3.3.18
LinkDB

KEGG   REACTION: R08870
Entry
R08870                      Reaction                               
Name
acetyl-CoA:phosphinopyruvate C-acetyltransferase (thioester-hydrolysing, carboxymethyl forming)
Definition
3-(Hydrohydroxyphosphoryl)pyruvate + Acetyl-CoA + H2O <=> 2-Phosphinomethylmalate + CoA
Equation
C06368 + C00024 + C00001 <=> C17947 + C00010
Comment
2-phosphinomethylmalic acid synthase
Reaction class
RC00004  C00010_C00024
RC00067  C06368_C17947
Enzyme
2.3.3.18
Pathway
rn00440  Phosphonate and phosphinate metabolism
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.3  Acyltransferases
   2.3.3  Acyl groups converted into alkyl groups on transfer
    2.3.3.18
     R08870  3-(Hydrohydroxyphosphoryl)pyruvate + Acetyl-CoA + H2O <=> 2-Phosphinomethylmalate + CoA
Orthology
K12910  2-phosphinomethylmalate synthase [EC:2.3.3.18]
LinkDB

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