KEGG ORTHOLOGY: K13272

ORTHOLOGY: K13272

Entry

K13272                      KO

Symbol

AOMT, CROMT2

Name

flavonoid O-methyltransferase [EC:2.1.1.267]

Pathway

map00944

Flavone and flavonol biosynthesis

map01110

Biosynthesis of secondary metabolites

Reaction

R06815

S-adenosyl-L-methionine:myricetin O-methyltransferase

R06816

S-adenosyl-L-methionine:myricetin O-methyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00944 Flavone and flavonol biosynthesis
    K13272  AOMT, CROMT2; flavonoid O-methyltransferase
Enzymes [BR:ko01000]
2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.267  flavonoid 3',5'-methyltransferase
     K13272  AOMT, CROMT2; flavonoid O-methyltransferase

Other DBs

GO:	0033799 0070448

Genes

TCC:

GRA:

GHI:

GAB:

HSYR:

120117959 120138721 120179562 120197908

DZI:	111282216 111288157 111317993

EGR:	104415685 104415688 104415689 104417751

RARG:

115738850

FVE:	101297250 101302900 101307171

AANS:

126795993 126795995

RCN:	112181235 112201013

PPER:

PMUM:

PAVI:

PDUL:

MDM:	103403159 103452136

MSYL:

126595165 126611729

PXB:	103929948 103961472 103965012

PCOX:

137731728 137732533 137736493 137748436

ZJU:

107428351

CSAV:

115712602 115712603 115712971 115712973

JRE:

CILL:

CAVE:

AGLU:

QSU:	112011645 112011654 112017664 112017707 136065185 136066073

QLO:	115982101 115982433

VVI:	100233134(FAOMT) 100245452 100250567 100250579

VRI:	117911684 117922688 117923246

SLY:	101255734(AnthOMT)

SPEN:

107031250

SOT:	102599242(AOMT3)

SSTN:

125851865

LBB:

132617324

SIND:

105156703 105168404 105168406 105168407

OEU:	111391869 111393471 111393476

EGT:	105950082 105952615 105952640 105958570 105958578 105958584 105970959

SSPL:

121793763 121798458 121802564 121802888 121809514

SMIL:

130995613 130995615

SHIS:

125198982 125200483 125204768 125211759 125211845 125217009

APAN:

127241181 127241183 127244939

HPUM:

140871705 140871825

PHUA:

140982457

PEBU:

140808260 140808261 140817306 140830010 140840494

CSIN:

114275659 114293531 114305583 114312686 114317032

RVL:	131311302 131311303 131311304

AEW:	130771302 130771738

DLT:

127796891

ATRI:

130809233 130809234

SLAF:

141586205 141623483 141637806

NNU:

104593275

MING:

122079574

TSS:	122647755 122652785 122652787 122663698

CJF:

131038561

AG:	AAM97497(OMT2)

» show all

Reference

PMID:12482447

Authors

Cacace S, Schroder G, Wehinger E, Strack D, Schmidt J, Schroder J

Title

A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations.

Journal

Phytochemistry 62:127-37 (2003)
DOI:10.1016/S0031-9422(02)00483-1

Sequence

[ag:AAM97497]

Reference

PMID:19525322

Authors

Hugueney P, Provenzano S, Verries C, Ferrandino A, Meudec E, Batelli G, Merdinoglu D, Cheynier V, Schubert A, Ageorges A

Title

A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine.

Journal

Plant Physiol 150:2057-70 (2009)
DOI:10.1104/pp.109.140376

Sequence

[vvi:100233134]

LinkDB

ENZYME: 2.1.1.267

Entry

EC 2.1.1.267                Enzyme

Name

flavonoid 3',5'-methyltransferase;
AOMT;
CrOMT2

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:flavonoid 3'-O-methyltransferase

Reaction(IUBMB)

(1) S-adenosyl-L-methionine + a 3'-hydroxyflavonoid = S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid;
(2) S-adenosyl-L-methionine + a 5'-hydroxy-3'-methoxyflavonoid = S-adenosyl-L-homocysteine + a 3',5'-dimethoxyflavonoid

Reaction(KEGG)

(other) R06815 R06816

Substrate

S-adenosyl-L-methionine [CPD:C00019];
3'-hydroxyflavonoid [CPD:C02790];
5'-hydroxy-3'-methoxyflavonoid

Product

S-adenosyl-L-homocysteine [CPD:C00021];
3'-methoxyflavonoid;
3',5'-dimethoxyflavonoid

Comment

Isolated from Vitis vinifera (grape) [2]. Most active with delphinidin 3-glucoside but also acts on cyanidin 3-glucoside, cyanidin, myricetin, quercetin and quercetin 3-glucoside. The enzyme from Catharanthus roseus was most active with myricetin [1].

History

EC 2.1.1.267 created 2013, modified 2014

Pathway

ec00944

Flavone and flavonol biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K13272

flavonoid O-methyltransferase

Genes

TCC:

18609023

GRA:

105785009

GHI:

107933317

GAB:

108458342

HSYR:

120117959 120138721 120179562 120197908

DZI:	111282216 111288157 111317993

EGR:	104415685 104415688 104415689 104417751

RARG:

115738850

FVE:	101297250 101302900 101307171

AANS:

126795993 126795995

RCN:	112181235 112201013

PPER:

109947186 18793505

PMUM:

103322058 103331367

PAVI:

110756049 110769740

PDUL:

117616544 117619928

MDM:	103403159 103452136

MSYL:

126595165 126611729

PXB:	103929948 103961472 103965012

PCOX:

137731728 137732533 137736493 137748436

ZJU:

107428351

CSAV:

115712602 115712603 115712971 115712973

JRE:

109003166

CILL:

122309323

CAVE:

132192083

AGLU:

133864727

QSU:	112011645 112011654 112017664 112017707 136065185 136066073

QLO:	115982101 115982433

VVI:	100233134(FAOMT) 100245452 100250567 100250579

VRI:	117911684 117922688 117923246

SLY:	101255734(AnthOMT)

SPEN:

107031250

SOT:	102599242(AOMT3)

SSTN:

125851865

LBB:

132617324

SIND:

105156703 105168404 105168406 105168407

OEU:	111391869 111393471 111393476

EGT:	105950082 105952615 105952640 105958570 105958578 105958584 105970959

SSPL:

121793763 121798458 121802564 121802888 121809514

SMIL:

130995613 130995615

SHIS:

125198982 125200483 125204768 125211759 125211845 125217009

APAN:

127241181 127241183 127244939

HPUM:

140871705 140871825

PHUA:

140982457

PEBU:

140808260 140808261 140817306 140830010 140840494

CSIN:

114275659 114293531 114305583 114312686 114317032

RVL:	131311302 131311303 131311304

AEW:	130771302 130771738

DLT:

127796891

ATRI:

130809233 130809234

SLAF:

141586205 141623483 141637806

NNU:

104593275

MING:

122079574

TSS:	122647755 122652785 122652787 122663698

CJF:

131038561

» show all

Reference

1 [PMID:12482447]

Authors

Cacace S, Schroder G, Wehinger E, Strack D, Schmidt J, Schroder J

Title

A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations.

Journal

Phytochemistry 62:127-37 (2003)
DOI:10.1016/S0031-9422(02)00483-1

Sequence

[ag:AAM97497]

Reference

2 [PMID:19525322]

Authors

Hugueney P, Provenzano S, Verries C, Ferrandino A, Meudec E, Batelli G, Merdinoglu D, Cheynier V, Schubert A, Ageorges A

Title

A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine.

Journal

Plant Physiol 150:2057-70 (2009)
DOI:10.1104/pp.109.140376

Sequence

[vvi:100233134]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.267

IUBMB Enzyme Nomenclature:

2.1.1.267

ExPASy - ENZYME nomenclature database:

2.1.1.267

BRENDA, the Enzyme Database:

2.1.1.267

LinkDB

REACTION: R06815

Entry

R06815                      Reaction

Name

S-adenosyl-L-methionine:myricetin O-methyltransferase

Definition

Myricetin + S-Adenosyl-L-methionine <=> Laricitrin + S-Adenosyl-L-homocysteine

Equation

C10107 + C00019 <=> C12633 + C00021

Reaction class

RC00003

C00019_C00021

RC00392

C10107_C12633

Enzyme

2.1.1.267

Pathway

rn00944

Flavone and flavonol biosynthesis

rn01110

Biosynthesis of secondary metabolites

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.267
     R06815  Myricetin + S-Adenosyl-L-methionine <=> Laricitrin + S-Adenosyl-L-homocysteine

Orthology

K13272

flavonoid O-methyltransferase [EC:2.1.1.267]

Other DBs

RHEA:

25632

LinkDB

DBGET integrated database retrieval system