KEGG   ORTHOLOGY: K13272
Entry
K13272                      KO                                     
Symbol
AOMT, CROMT2
Name
flavonoid O-methyltransferase [EC:2.1.1.267]
Pathway
map00944  Flavone and flavonol biosynthesis
map01110  Biosynthesis of secondary metabolites
Reaction
R06815  S-adenosyl-L-methionine:myricetin O-methyltransferase
R06816  S-adenosyl-L-methionine:myricetin O-methyltransferase
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09110 Biosynthesis of other secondary metabolites
   00944 Flavone and flavonol biosynthesis
    K13272  AOMT, CROMT2; flavonoid O-methyltransferase
Enzymes [BR:ko01000]
 2. Transferases
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.267  flavonoid 3',5'-methyltransferase
     K13272  AOMT, CROMT2; flavonoid O-methyltransferase
Other DBs
GO: 0033799 0070448
Genes
TCC: 18609023
GRA: 105785009
GHI: 107933317
GAB: 108458342
HSYR: 120117959 120138721 120179562 120197908
DZI: 111282216 111288157 111317993
EGR: 104415685 104415688 104415689 104417751
RARG: 115738850
FVE: 101297250 101302900 101307171
AANS: 126795993 126795995
ZJU: 107428351
JRE: 109003166
CILL: 122309323
CAVE: 132192083
AGLU: 133864727
SLY: 101255734(AnthOMT)
SPEN: 107031250
SOT: 102599242(AOMT3)
SSTN: 125851865
LBB: 132617324
PHUA: 140982457
DLT: 127796891
NNU: 104593275
MING: 122079574
CJF: 131038561
AG: AAM97497(OMT2)
 » show all
Reference
  Authors
Cacace S, Schroder G, Wehinger E, Strack D, Schmidt J, Schroder J
  Title
A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations.
  Journal
Phytochemistry 62:127-37 (2003)
DOI:10.1016/S0031-9422(02)00483-1
  Sequence
Reference
  Authors
Hugueney P, Provenzano S, Verries C, Ferrandino A, Meudec E, Batelli G, Merdinoglu D, Cheynier V, Schubert A, Ageorges A
  Title
A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine.
  Journal
Plant Physiol 150:2057-70 (2009)
DOI:10.1104/pp.109.140376
  Sequence
[vvi:100233134]
LinkDB

KEGG   ENZYME: 2.1.1.267
Entry
EC 2.1.1.267                Enzyme                                 
Name
flavonoid 3',5'-methyltransferase;
AOMT;
CrOMT2
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:flavonoid 3'-O-methyltransferase
Reaction(IUBMB)
(1) S-adenosyl-L-methionine + a 3'-hydroxyflavonoid = S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid;
(2) S-adenosyl-L-methionine + a 5'-hydroxy-3'-methoxyflavonoid = S-adenosyl-L-homocysteine + a 3',5'-dimethoxyflavonoid
Reaction(KEGG)
(other) R06815 R06816
Substrate
S-adenosyl-L-methionine [CPD:C00019];
3'-hydroxyflavonoid [CPD:C02790];
5'-hydroxy-3'-methoxyflavonoid
Product
S-adenosyl-L-homocysteine [CPD:C00021];
3'-methoxyflavonoid;
3',5'-dimethoxyflavonoid
Comment
Isolated from Vitis vinifera (grape) [2]. Most active with delphinidin 3-glucoside but also acts on cyanidin 3-glucoside, cyanidin, myricetin, quercetin and quercetin 3-glucoside. The enzyme from Catharanthus roseus was most active with myricetin [1].
History
EC 2.1.1.267 created 2013, modified 2014
Pathway
ec00944  Flavone and flavonol biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13272  flavonoid O-methyltransferase
Genes
TCC18609023
GRA105785009
GHI107933317
GAB108458342
HSYR120117959 120138721 120179562 120197908
DZI111282216 111288157 111317993
EGR104415685 104415688 104415689 104417751
RARG115738850
FVE101297250 101302900 101307171
AANS126795993 126795995
RCN112181235 112201013
PPER109947186 18793505
PMUM103322058 103331367
PAVI110756049 110769740
PDUL117616544 117619928
MDM103403159 103452136
MSYL126595165 126611729
PXB103929948 103961472 103965012
PCOX137731728 137732533 137736493 137748436
ZJU107428351
CSAV115712602 115712603 115712971 115712973
JRE109003166
CILL122309323
CAVE132192083
AGLU133864727
QSU112011645 112011654 112017664 112017707 136065185 136066073
QLO115982101 115982433
VVI100233134(FAOMT) 100245452 100250567 100250579
VRI117911684 117922688 117923246
SLY101255734(AnthOMT)
SPEN107031250
SOT102599242(AOMT3)
SSTN125851865
LBB132617324
SIND105156703 105168404 105168406 105168407
OEU111391869 111393471 111393476
EGT105950082 105952615 105952640 105958570 105958578 105958584 105970959
SSPL121793763 121798458 121802564 121802888 121809514
SMIL130995613 130995615
SHIS125198982 125200483 125204768 125211759 125211845 125217009
APAN127241181 127241183 127244939
HPUM140871705 140871825
PHUA140982457
PEBU140808260 140808261 140817306 140830010 140840494
CSIN114275659 114293531 114305583 114312686 114317032
RVL131311302 131311303 131311304
AEW130771302 130771738
DLT127796891
ATRI130809233 130809234
SLAF141586205 141623483 141637806
NNU104593275
MING122079574
TSS122647755 122652785 122652787 122663698
CJF131038561
 » show all
Reference
1  [PMID:12482447]
  Authors
Cacace S, Schroder G, Wehinger E, Strack D, Schmidt J, Schroder J
  Title
A flavonol O-methyltransferase from Catharanthus roseus performing two sequential methylations.
  Journal
Phytochemistry 62:127-37 (2003)
DOI:10.1016/S0031-9422(02)00483-1
  Sequence
Reference
2  [PMID:19525322]
  Authors
Hugueney P, Provenzano S, Verries C, Ferrandino A, Meudec E, Batelli G, Merdinoglu D, Cheynier V, Schubert A, Ageorges A
  Title
A novel cation-dependent O-methyltransferase involved in anthocyanin methylation in grapevine.
  Journal
Plant Physiol 150:2057-70 (2009)
DOI:10.1104/pp.109.140376
  Sequence
[vvi:100233134]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.267
IUBMB Enzyme Nomenclature: 2.1.1.267
ExPASy - ENZYME nomenclature database: 2.1.1.267
BRENDA, the Enzyme Database: 2.1.1.267
LinkDB

KEGG   REACTION: R06815
Entry
R06815                      Reaction                               
Name
S-adenosyl-L-methionine:myricetin O-methyltransferase
Definition
Myricetin + S-Adenosyl-L-methionine <=> Laricitrin + S-Adenosyl-L-homocysteine
Equation
Reaction class
RC00003  C00019_C00021
RC00392  C10107_C12633
Enzyme
Pathway
rn00944  Flavone and flavonol biosynthesis
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 2. Transferase reactions
  2.1  Transferring one-carbon groups
   2.1.1  Methyltransferases
    2.1.1.267
     R06815  Myricetin + S-Adenosyl-L-methionine <=> Laricitrin + S-Adenosyl-L-homocysteine
Orthology
K13272  flavonoid O-methyltransferase [EC:2.1.1.267]
Other DBs
RHEA: 25632
LinkDB

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