Escherichia coli BL21-Gold(DE3)pLysS AG: ECBD_2622
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Entry
ECBD_2622 CDS
T00939
Name
(GenBank) nickel-dependent hydrogenase large subunit
KO
K06281
hydrogenase large subunit [EC:
1.12.99.6
]
Organism
ebd
Escherichia coli BL21-Gold(DE3)pLysS AG
Pathway
ebd00633
Nitrotoluene degradation
ebd01120
Microbial metabolism in diverse environments
ebd02020
Two-component system
Brite
KEGG Orthology (KO) [BR:
ebd00001
]
09100 Metabolism
09111 Xenobiotics biodegradation and metabolism
00633 Nitrotoluene degradation
ECBD_2622
09130 Environmental Information Processing
09132 Signal transduction
02020 Two-component system
ECBD_2622
Enzymes [BR:
ebd01000
]
1. Oxidoreductases
1.12 Acting on hydrogen as donor
1.12.99 With unknown physiological acceptors
1.12.99.6 hydrogenase (acceptor)
ECBD_2622
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GFIT
Motif
Pfam:
NiFeSe_Hases
Motif
Other DBs
NCBI-ProteinID:
ACT29644
UniProt:
A0A140NB83
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Position
complement(2742249..2744042)
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AA seq
597 aa
AA seq
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MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVITNAVSCGTMFRGLEIIL
QGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDNANIIRNIMLATLWCHDHL
VHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSPGYFFDVQNRLKKFVEGGQLG
IFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVKIHAVFGGKNPHPNWIVGGMPCA
INIDESGAVGAVNMERLNLVQSIITRTADFINNVMIPDALAIGQFNKPWSEIGTGLSDKC
VLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNVLPVDLVDPQQVQEFVDHAWYRYPNDQ
VGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQERYSWIKAPRWRGNAMEVGPLARTLIAY
HKGDAATVESVDRMMSALNLPLSGIQSTLGRILCRAHEAQWAAGKLQYFFDKLMTNLKNG
NLATASTEKWEPATWPTECRGVGFTEAPRGALGHWAAIRDGKIDLYQCVVPTTWNASPRD
PKGQIGAYEAALMNTKMAIPEQPLEILRTLHSFDPCLACSTHVLGDDGSELISVQVR
NT seq
1794 nt
NT seq
+upstream
nt +downstream
nt
atgagcactcagtacgaaactcagggatacaccatcaataatgccggacgccgcctggtg
gtcgacccgattacgcgcatcgaaggccacatgcgctgcgaagtgaatattaacgatcag
aatgtgatcaccaatgccgtctcctgcggcaccatgtttcgcgggctggagatcatccta
caagggcgcgacccgcgcgatgcgtgggcgttcgttgaacgtatctgcggcgtctgtact
ggcgtacacgccctggcttcggtttacgccatcgaagatgctatcggtattaaagtgccg
gacaacgccaatatcatccgcaacattatgctggcaacgctctggtgccacgatcatctg
gtgcacttctatcagcttgccgggatggactggatcgatgtgttagatgcgctgaaagcc
gacccgcggaaaacctccgaactggcgcaaagtctctcctcttggccgaaatcatcccct
ggctatttcttcgacgtacaaaaccgcctgaaaaaatttgttgaaggcgggcagttgggg
atcttccgcaatggctactgggggcacccgcagtacaaactgccgccagaagctaacctg
atgggctttgcccactatctcgaagctctcgatttccagcgtgaaattgtcaaaatccac
gcggtctttggcggtaaaaacccgcatccaaactggattgtcggcgggatgccttgcgcc
atcaacattgacgaaagcggcgcggtcggggcagtcaatatggaacgcctgaacctggtg
cagtcaattatcacccgcacggcggacttcattaacaacgtgatgatccccgacgcctta
gccatcggtcagttcaacaaaccgtggagcgaaatcggcactggtctttctgataaatgc
gttctcagctacggcgcattcccggatattgccaacgactttggcgagaaaagtctgctg
atgcctggcggcgcggtgattaacggcgacttcaacaatgtgctgccagtggatttggtt
gatccgcagcaggtgcaggagtttgtcgaccacgcctggtatcgatatcccaacgatcag
gtcgggcgtcatccgttcgatggcatcaccgacccgtggtacaaccccggcgatgtcaaa
ggcagcgataccaacattcagcagctgaatgaacaggaacgctactcgtggatcaaagcg
ccacgctggcgcggtaacgcgatggaagtggggccgctggcgcgcacgttaatcgcttat
cacaaaggcgatgctgcgaccgttgagtcggtcgatcgcatgatgtcggcgttgaacctg
ccgctttccggtatccagtcaacgttaggccgcattttgtgccgcgcgcacgaagcgcag
tgggccgcaggtaagttgcagtatttcttcgacaagctgatgaccaacctgaaaaacggc
aatctcgccactgcttccacggaaaaatgggaacctgcaacctggccgacagagtgccgt
ggtgtcggttttaccgaagcgccgcgcggggcgttaggccactgggccgccattcgcgat
ggcaagattgatctctaccagtgcgtggtgccgaccacctggaacgccagcccgcgcgat
cccaaagggcagattggcgcttatgaagcggcgctgatgaacaccaaaatggcgatcccc
gagcaaccgctggagatcctgcgtactctgcacagctttgacccgtgcctcgcctgttca
acacacgtgctgggcgacgacggtagcgagctgatctccgtgcaggtgcgttaa
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