KEGG   ENZYME: 1.1.1.283
Entry
EC 1.1.1.283                Enzyme                                 

Name
methylglyoxal reductase (NADPH);
lactaldehyde dehydrogenase (NADP+);
GRE2 (gene name);
methylglyoxal reductase (NADPH-dependent);
lactaldehyde:NADP+ oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(S)-lactaldehyde:NADP+ oxidoreductase
Reaction(IUBMB)
(S)-lactaldehyde + NADP+ = 2-oxopropanal + NADPH + H+ [RN:R02260]
Reaction(KEGG)
R02260
Substrate
(S)-lactaldehyde [CPD:C00424];
NADP+ [CPD:C00006]
Product
2-oxopropanal [CPD:C00546];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme from the yeast Saccharomyces cerevisiae catalyses the reduction of a keto group in a number of compounds, forming enantiopure products. Among the substrates are methylglyoxal (which is reduced to (S)-lactaldehyde) [1,2], 3-methylbutanal [3], hexane-2,5-dione [4] and 3-chloro-1-phenylpropan-1-one [5]. The enzyme differs from EC 1.1.1.78, methylglyoxal reductase (NADH), which is found in mammals, by its coenzyme requirement, reaction direction, and enantiomeric preference.
History
EC 1.1.1.283 created 2005, modified 2013
Pathway
ec00620  Pyruvate metabolism
ec00640  Propanoate metabolism
ec01100  Metabolic pathways
Orthology
K17741  NADPH-dependent methylglyoxal reductase
K23257  methylglyoxal/glyoxal reductase
Genes
SCE: YOL151W(GRE2)
KLA: KLLA0_D11000g
KMX: KLMA_30018(ARI1) KLMA_40628 KLMA_60425(GRE2) KLMA_80004 KLMA_80060
LTH: KLTH0B06182g KLTH0B06402g KLTH0E09680g KLTH0F04026g
VPO: Kpol_1030p19 Kpol_1057p21 Kpol_416p7 Kpol_529p27
ZRO: ZYRO0A13574g ZYRO0B00330g ZYRO0B16742g ZYRO0C18546g ZYRO0E10362g ZYRO0F05214g ZYRO0F18656g ZYRO0G00242g ZYRO0G15400g ZYRO0G22506g
CGR: CAGL0E05170g CAGL0E05280g
NCS: NCAS_0A03090(NCAS0A03090) NCAS_0A03400(NCAS0A03400) NCAS_0A07210(NCAS0A07210) NCAS_0C05270(NCAS0C05270) NCAS_0C05980(NCAS0C05980) NCAS_0D02420(NCAS0D02420) NCAS_0D03480(NCAS0D03480) NCAS_0D04850(NCAS0D04850) NCAS_0G03820(NCAS0G03820) NCAS_0H00790(NCAS0H00790)
NDI: NDAI_0D00910(NDAI0D00910) NDAI_0D05090(NDAI0D05090) NDAI_0F01560(NDAI0F01560) NDAI_0I02450(NDAI0I02450)
TPF: TPHA_0A00590(TPHA0A00590) TPHA_0F03690(TPHA0F03690) TPHA_0M01160(TPHA0M01160) TPHA_0N01940(TPHA0N01940) TPHA_0O00640(TPHA0O00640) TPHA_0O01220(TPHA0O01220)
TBL: TBLA_0A01590(TBLA0A01590) TBLA_0C00270(TBLA0C00270) TBLA_0C00280(TBLA0C00280) TBLA_0C03410(TBLA0C03410) TBLA_0C03420(TBLA0C03420) TBLA_0C03430(TBLA0C03430) TBLA_0D00750(TBLA0D00750)
TDL: TDEL_0A00160(TDEL0A00160) TDEL_0C00230(TDEL0C00230) TDEL_0C02800(TDEL0C02800) TDEL_0D00270(TDEL0D00270)
KAF: KAFR_0A08710(KAFR0A08710) KAFR_0A08720(KAFR0A08720) KAFR_0C00150(KAFR0C00150)
PIC: PICST_32463(GRP3.1) PICST_58065(GRP3.3) PICST_72153(GRE2)
CAL: CAALFM_C205560WA(CaO19.6868) CAALFM_C206710WA(CaO19.3151) CAALFM_C206720WA(GRE2) CAALFM_C502860CA(GRP2) CAALFM_C603240WA(CaO19.5611) CAALFM_CR01810CA(CaO19.2581)
BSU: BSU29050(ytbE) BSU33400(yvgN)
BSH: BSU6051_29050(ytbE) BSU6051_33400(yvgN)
BSUT: BSUB_03093(ytbE) BSUB_03568(yvgN)
BSUL: BSUA_03093(ytbE) BSUA_03568(yvgN)
BSS: BSUW23_14120(ytbE) BSUW23_16365(yvgN)
BSO: BSNT_09325(ytbE) BSNT_09856(yvgN)
BSQ: B657_29050(ytbE) B657_33400(yvgN)
BSX: C663_2750(ytbE) C663_3209(yvgN)
BLI: BL00471(ytbE) BL00701(yvgN)
BLD: BLi02838(ytbE) BLi03528(yvgN)
BLH: BaLi_c29370(ytbE) BaLi_c35910(yvgN)
BAQ: BACAU_2626(ytbE) BACAU_3082(yvgN)
BYA: BANAU_2824(ytbE) BANAU_3247(yvgN)
BAML: BAM5036_2549(ytbE) BAM5036_2970(yvgN)
BAMA: RBAU_2746(ytbE) RBAU_3189(yvgN)
BAMN: BASU_2552(ytbE) BASU_2975(yvgN)
BAMB: BAPNAU_0944(ytbE) BAPNAU_3254(yvgN)
BAMY: V529_28970(ytbE) V529_33190
BMP: NG74_02751(ytbE) NG74_03226(yvgN)
BAO: BAMF_2708(ytbE) BAMF_3187(yvgN)
BAZ: BAMTA208_14265(ytbE) BAMTA208_16930(yvgN)
BQL: LL3_02993(ytbE) LL3_03470(yvgN)
BXH: BAXH7_02921(ytbE) BAXH7_03457(yvgN)
BQY: MUS_3180(ytbE) MUS_3654(yvgN)
BTG: BTB_c02190(yvgN1) BTB_c42340(yvgN2) BTB_c52890
BWW: bwei_0852(yvgN) bwei_4697(dkgA) bwei_5541
BACO: OXB_0365
BACL: BS34A_31680(ytbE) BS34A_36460(yvgN)
BGY: BGLY_3155(ytbE) BGLY_3917(yvgN)
BFD: NCTC4823_02898(yvgN)
OIH: OB2664
GKA: GK0562
GGH: GHH_c05420(ytbE)
GEA: GARCT_00563(yvgN)
AFL: Aflv_1208
AAMY: GFC30_947
AXL: AXY_22140
LSP: Bsph_0089(ytbE) Bsph_4482
HLI: HLI_12560
VPN: A21D_02538(yvgN)
PSYO: PB01_10775
BSE: Bsel_1577
LMO: lmo0823
LMOE: BN418_0968
LMOB: BN419_0972
LMOD: LMON_0827
LMOW: AX10_12655
LMOQ: LM6179_1137(yvgN)
LMR: LMR479A_0841(yvgN)
LMOM: IJ09_06215
LMOG: BN389_08530(yvgN)
LMP: MUO_04385
LMOX: AX24_01430
LMQ: LMM7_0854(dkgA)
LMS: LMLG_2493
LMOK: CQ02_04305
LIN: lin0819
LWE: lwe0817
LSG: lse_0721
LIV: LIV_0760
LGZ: NCTC10812_02018(yvgN_2) NCTC10812_02121(ytbE)
BLR: BRLA_c006250(ytbE_1) BRLA_c009840 BRLA_c046180(ytbE_2)
PPM: PPSC2_01645(ytbE) PPSC2_04330(yvgN)
PPO: PPM_0311(ytbE) PPM_0809(yvgN)
PMS: KNP414_03688(yvgN) KNP414_07911(ytbE)
PRI: PRIO_2517(yvgN1) PRIO_5501(ytbE1) PRIO_6536(ytbE3)
PSWU: SY83_16510
PBK: Back11_54940(ytbE)
ASOC: CB4_02589(yvgN)
COHN: KCTCHS21_58600(ytbE)
AAC: Aaci_0848
AAD: TC41_0849
BTS: Btus_0334
SIV: SSIL_1990
LLA: L132617(yddB)
LLK: LLKF_0388(yddB)
LLT: CVCAS_0318(yddB)
LLS: lilo_0300(yddB)
LLM: llmg_0352
LLC: LACR_0378
LLR: llh_1960
LLW: kw2_0336
LLJ: LG36_0336(yddB)
LSL: LSL_1681
LSI: HN6_01406
LSJ: LSJ_1722c
PPE: PEPE_0025
PPEN: T256_00125
EFI: OG1RF_10362(morA) OG1RF_10915(dkgB)
ENE: ENT_24880
EHR: EHR_10290
ECAS: ECBG_03152
MPS: MPTP_1009
MPX: MPD5_0938
WKO: WKK_05705
WCE: WS08_1257
WCT: WS74_1328
CML: BN424_3458(ytbE)
CAC: CA_C1958
CAE: SMB_G1990(yvgN)
CAY: CEA_G1975
CBO: CBO0984(dkgA)
CBA: CLB_1023
CBH: CLC_1037
CBY: CLM_1138
CBL: CLK_0430
CBK: CLL_A1844
CBB: CLD_3583
CBI: CLJ_B1026
CBT: CLH_1658
CBF: CLI_1068
CBM: CBF_1038
CPAS: Clopa_4262
ASF: SFBM_0648
PUF: UFO1_0239
PFT: JBW_01307
FIT: Fi14EGH31_27840(morA)
GFO: GFO_0840
GFL: GRFL_2730
ZPR: ZPR_1260
MARM: YQ22_08605
MLT: VC82_1415
SPON: HME9304_01592(dkgA)
PMO: Pmob_1501
DTN: DTL3_1551(yvgN1)
 » show all
Reference
1  [PMID:3896793]
  Authors
Murata K, Fukuda Y, Simosaka M, Watanabe K, Saikusa T, Kimura A.
  Title
Metabolism of 2-oxoaldehyde in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae.
  Journal
Eur J Biochem 151:631-6 (1985)
DOI:10.1111/j.1432-1033.1985.tb09151.x
  Sequence
[sce:YOL151W]
Reference
2  [PMID:12722185]
  Authors
Chen CN, Porubleva L, Shearer G, Svrakic M, Holden LG, Dover JL, Johnston M, Chitnis PR, Kohl DH
  Title
Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae.
  Journal
Yeast 20:545-54 (2003)
DOI:10.1002/yea.979
  Sequence
[sce:YOL151W]
Reference
3  [PMID:16999827]
  Authors
Hauser M, Horn P, Tournu H, Hauser NC, Hoheisel JD, Brown AJ, Dickinson JR
  Title
A transcriptome analysis of isoamyl alcohol-induced filamentation in yeast reveals a novel role for Gre2p as isovaleraldehyde reductase.
  Journal
FEMS Yeast Res 7:84-92 (2007)
DOI:10.1111/j.1567-1364.2006.00151.x
  Sequence
[sce:YOL151W]
Reference
4  [PMID:20237665]
  Authors
Muller M, Katzberg M, Bertau M, Hummel W
  Title
Highly efficient and stereoselective biosynthesis of (2S,5S)-hexanediol with a dehydrogenase from Saccharomyces cerevisiae.
  Journal
Org Biomol Chem 8:1540-50 (2010)
DOI:10.1039/b920869k
  Sequence
[sce:YOL151W]
Reference
5  [PMID:20111861]
  Authors
Choi YH, Choi HJ, Kim D, Uhm KN, Kim HK
  Title
Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity.
  Journal
Appl Microbiol Biotechnol 87:185-93 (2010)
DOI:10.1007/s00253-010-2442-5
  Sequence
[sce:YOL151W]
Reference
6  [PMID:20606287]
  Authors
Breicha K, Muller M, Hummel W, Niefind K
  Title
Crystallization and preliminary crystallographic analysis of Gre2p, an NADP(+)-dependent alcohol dehydrogenase from Saccharomyces cerevisiae.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 66:838-41 (2010)
DOI:10.1107/S1744309110018889
  Sequence
[sce:YOL151W]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.283
IUBMB Enzyme Nomenclature: 1.1.1.283
ExPASy - ENZYME nomenclature database: 1.1.1.283
BRENDA, the Enzyme Database: 1.1.1.283
CAS: 78310-66-4
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