KEGG   ENZYME: 1.1.1.292
Entry
EC 1.1.1.292                Enzyme                                 

Name
1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming);
1,5-anhydro-D-fructose reductase (ambiguous);
AFR (ambiguous)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
1,5-anhydro-D-mannitol:NADP+ oxidoreductase
Reaction(IUBMB)
1,5-anhydro-D-mannitol + NADP+ = 1,5-anhydro-D-fructose + NADPH + H+ [RN:R08194]
Reaction(KEGG)
R08194
Substrate
1,5-anhydro-D-mannitol [CPD:C16538];
NADP+ [CPD:C00006]
Product
1,5-anhydro-D-fructose [CPD:C06485];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
This enzyme is present in some but not all Rhizobium species and belongs in the GFO/IDH/MocA protein family [2]. This enzyme differs from hepatic 1,5-anhydro-D-fructose reductase, which yields 1,5-anhydro-D-glucitol as the product (see EC 1.1.1.263). In Sinorhizobium morelense, the product of the reaction, 1,5-anhydro-D-mannitol, can be further metabolized to D-mannose [1]. The enzyme also reduces 1,5-anhydro-D-erythro-hexo-2,3-diulose and 2-ketoaldoses (called osones), such as D-glucosone (D-arabino-hexos-2-ulose) and 6-deoxy-D-glucosone. It does not reduce common aldoses and ketoses, or non-sugar aldehydes and ketones [1].
History
EC 1.1.1.292 created 2007
Orthology
K19181  1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)
Genes
DJI: CH75_01615
DJA: HY57_19505
DTX: ATSB10_21920
LRZ: BJI69_02960
LPY: FIV34_16705
PFL: PFL_3704
PPRC: PFLCHA0_c37490(afr)
PPRO: PPC_3847
PFO: Pfl01_3165
PFC: PflA506_2436
PMAN: OU5_0029
PSOS: POS17_3866
PANR: A7J50_2502
VEI: Veis_3569
LCH: Lcho_4310
CCX: COCOR_00217(afr)
MHUA: MCHK_4557
AMIH: CO731_02111(afr_2)
SME: SMc04400
SMX: SM11_chr3428(afr)
SMI: BN406_03101(afr)
SMEL: SM2011_c04400(afr)
SMER: DU99_17800
SMD: Smed_3174
SFD: USDA257_c58200(afr)
EAD: OV14_0832
RGA: RGR602_CH02580(afr)
BME: BMEI0020
BMEL: DK63_1414(afr)
BMEE: DK62_1534(afr)
BMF: BAB1_2049
BABO: DK55_1982(afr)
BABR: DO74_1993(afr)
BABT: DK49_1739(afr)
BABB: DK48_137(afr)
BABU: DK53_1967(afr)
BABS: DK51_1575(afr)
BABC: DO78_1886(afr)
BMS: BR2048
BSZ: DK67_318(afr)
BOV: BOV_1970
BCAR: DK60_2024(afr)
BCAS: DA85_09845
BMR: BMI_I2070
BPP: BPI_I2106
BPV: DK65_1488(afr)
OAN: Oant_0871
OAH: DR92_409(afr)
OCH: CES85_2562(afr)
MDI: METDI3441
MEX: Mext_2682
MCH: Mchl_2909
MPO: Mpop_2804
MET: M446_2288
MOR: MOC_4117
META: Y590_12890
MAQU: Maq22A_1p30425(mviM)
BID: Bind_0626
MMED: Mame_00846(afr_2)
JAN: Jann_3940
MALG: MALG_00773
LABT: FIU93_08820(afr1)
RID: RIdsm_04030(afr)
GXY: GLX_01130
KSC: CD178_03511(afr)
PMW: B2K_32355
SIV: SSIL_0513
CTC: CTC_00910
CPY: Cphy_2322
RER: RER_09450
ROP: ROP_22020
SFK: KY5_7290
MOY: CVS54_00213(gfo)
ART: Arth_1860
ACIJ: JS278_00456(iolX_1)
NML: Namu_4105
GOB: Gobs_1023
MMAR: MODMU_0874
KRA: Krad_3430
AMN: RAM_03135
AMM: AMES_0611(rifS)
AMZ: B737_0612(rifS)
TRA: Trad_0667
TTR: Tter_2602
BVO: Pan97_37330(afr_3)
FTJ: FTUN_0104
IPA: Isop_2920
ACA: ACP_2132
SUS: Acid_3185
ABAC: LuPra_02187(afr_3)
GBA: J421_4304
BTHO: Btheta7330_03056(afr_2)
BXY: BXY_15300
BOA: Bovatus_00164(afr)
BCEL: BcellWH2_04629(afr_2)
PMUC: ING2E5A_1728(afr)
MBAS: ALGA_3915
FAE: FAES_4139
ASAC: ATHSA_1604
 » show all
Reference
1  [PMID:16461673]
  Authors
Kuhn A, Yu S, Giffhorn F
  Title
Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis.
  Journal
Appl Environ Microbiol 72:1248-57 (2006)
DOI:10.1128/AEM.72.2.1248-1257.2006
  Sequence
Reference
2  [PMID:16906761]
  Authors
Dambe TR, Kuhn AM, Brossette T, Giffhorn F, Scheidig AJ
  Title
Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 A resolution: construction of a NADH-accepting mutant and its application in rare sugar synthesis.
  Journal
Biochemistry 45:10030-42 (2006)
DOI:10.1021/bi052589q
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.292
IUBMB Enzyme Nomenclature: 1.1.1.292
ExPASy - ENZYME nomenclature database: 1.1.1.292
UM-BBD (Biocatalysis/Biodegradation Database): 1.1.1.292
BRENDA, the Enzyme Database: 1.1.1.292
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