KEGG   ENZYME: 1.1.1.365Help
Entry
EC 1.1.1.365                Enzyme                                 

Name
D-galacturonate reductase;
GalUR;
gar1 (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
L-galactonate:NADP+ oxidoreductase
Reaction(IUBMB)
L-galactonate + NADP+ = D-galacturonate + NADPH + H+ [RN:R07676]
Reaction(KEGG)
Substrate
L-galactonate [CPD:C15930];
NADP+ [CPD:C00006]
Product
D-galacturonate [CPD:C00333];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme from plants is involved in ascorbic acid (vitamin C) biosynthesis [1,2]. The enzyme from the fungus Trichoderma reesei (Hypocrea jecorina) is involved in a eukaryotic degradation pathway of D-galacturonate. It is also active with D-glucuronate and glyceraldehyde [3]. Neither enzyme shows any activity with NADH.
History
EC 1.1.1.365 created 2013
Pathway
ec00040  Pentose and glucuronate interconversions
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
Orthology
K19634  D-galacturonate reductase
K19642  D-galacturonate reductase
Genes
TCC: 18609968 18609969 18609970
GRA: 105794339
GHI: 107909951 107948305
GAB: 108488709
DZI: 111282964 111287841 111318131
EGR: 104432997
FVE: 101312822
RCN: 112179335 112180478 112197683
PPER: 18792656
PMUM: 103320996 103320997
MCHA: 111023026
CMAX: 111487459
CMOS: 111447573
CPEP: 111776980
HBR: 110634149
JRE: 108996990
SLY: 101256763
SPEN: 107016409
SOT: 102586306
NSY: 104245176
NTO: 104110610
NAU: 109216145
INI: 109166850
OEU: 111399339
NNU: 104588465
TRE: TRIREDRAFT_22004(gar1)
 » show all
Taxonomy
Reference
1  [PMID:13363799]
  Authors
ISHERWOOD FA, MAPSON LW
  Title
Biological synthesis of ascorbic acid: the conversion of derivatives of D-galacturonic acid into L-ascorbic acid by plant extracts.
  Journal
Biochem J 64:13-22 (1956)
Reference
2  [PMID:12524550]
  Authors
Agius F, Gonzalez-Lamothe R, Caballero JL, Munoz-Blanco J, Botella MA, Valpuesta V
  Title
Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase.
  Journal
Nat Biotechnol 21:177-81 (2003)
DOI:10.1038/nbt777
  Sequence
Reference
3  [PMID:16101307]
  Authors
Kuorelahti S, Kalkkinen N, Penttila M, Londesborough J, Richard P
  Title
Identification in the mold Hypocrea jecorina of the first fungal D-galacturonic acid reductase.
  Journal
Biochemistry 44:11234-40 (2005)
DOI:10.1021/bi050792f
  Sequence
Reference
4  [PMID:18768163]
  Authors
Martens-Uzunova ES, Schaap PJ
  Title
An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation.
  Journal
Fungal Genet Biol 45:1449-57 (2008)
DOI:10.1016/j.fgb.2008.08.002
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.365
IUBMB Enzyme Nomenclature: 1.1.1.365
ExPASy - ENZYME nomenclature database: 1.1.1.365
BRENDA, the Enzyme Database: 1.1.1.365
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