Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

sophoraflavanone-B,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)

sophoraflavanone B + [reduced NADPH---hemoprotein reductase] + O2 = leachianone G + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R08922]

R08922

sophoraflavanone B [CPD:C18023];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]

A membrane-bound cytochrome P-450 (heme-thiolate) protein that is associated with the endoplasmic reticulum [1,2]. This enzyme is specific for sophoraflavanone B as substrate. Along with EC 2.5.1.70 (naringenin 8-dimethylallyltransferase) and EC 2.5.1.71 (leachianone G 2''-dimethylallyltransferase), this enzyme forms part of the sophoraflavanone G biosynthetic pathway.

EC 1.14.14.142 created 2007 asEC 1.14.13.103, transferred 2018 to EC 1.14.14.142

1  [PMID:11672730]

Yamamoto H, Yatou A, Inoue K.

8-dimethylallylnaringenin 2'-hydroxylase, the crucial cytochrome P450 mono-oxygenase for lavandulylated flavanone formation in Sophora flavescens cultured cells.

Phytochemistry 58:671-6 (2001)
DOI:10.1016/S0031-9422(01)00270-9

2  [PMID:14551337]

Zhao P, Inoue K, Kouno I, Yamamoto H.

Characterization of leachianone G 2"-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures.

Plant Physiol 133:1306-13 (2003)
DOI:10.1104/pp.103.025213