ENZYME: 1.14.14.94
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Entry
EC 1.14.14.94 Enzyme
Name
leukotriene-B4 20-monooxygenase;
leukotriene-B4 20-hydroxylase;
leucotriene-B4 omega-hydroxylase;
LTB4 20-hydroxylase;
LTB4 omega-hydroxylase;
CYP4F2 (gene name);
CYP4F3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH---hemoprotein reductase] + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R03866
]
Reaction(KEGG)
R03866
Reaction
Substrate
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate [CPD:
C02165
];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024
];
O2 [CPD:
C00007
]
Product
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate [CPD:
C04853
];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161
];
H2O [CPD:
C00001
]
Comment
A cytochrome P-450 (heme-thiolate) protein found in mammals.
History
EC 1.14.14.94 created 1989 as EC 1.14.13.30, transferred 2018 to EC 1.14.14.94
Pathway
ec00590
Arachidonic acid metabolism
Orthology
K17726
phylloquinone omega-hydroxylase / docosahexaenoic acid omega-hydroxylase / leukotriene-B4 20-monooxygenase
Genes
HSA:
4051
(CYP4F3)
8529
(CYP4F2)
PTR:
100609185
748117
(CYP4F3)
748531
(CYP4F2)
PPS:
100967411
100969141
100969847
100982619
GGO:
101125692
101135418
101152881
PON:
100434096
100437847
100453229
NLE:
100579320
100588006
115836968
MCC:
718349
719317
MCF:
102130440
(CYP4F3v2)
102134301
(CYP4F45)
CSAB:
103234079
103234089
RRO:
104661012
104661014
RBB:
108543262
108543288
108543294
CJC:
100411160
100412575
(CYP4F2)
SBQ:
101050944
101051361
OCU:
100348038
100350957
TUP:
102483333
DRO:
112305042
» show all
Taxonomy
Reference
1 [PMID:
3027095
]
Authors
Romano MC, Eckardt RD, Bender PE, Leonard TB, Straub KM, Newton JF.
Title
Biochemical characterization of hepatic microsomal leukotriene B4 hydroxylases.
Journal
J Biol Chem 262:1590-5 (1987)
Reference
2 [PMID:
4044832
]
Authors
Shak S, Goldstein IM.
Title
Leukotriene B4 omega-hydroxylase in human polymorphonuclear leukocytes. Partial purification and identification as a cytochrome P-450.
Journal
J Clin Invest 76:1218-28 (1985)
DOI:
10.1172/JCI112077
Reference
3 [PMID:
2986111
]
Authors
Soberman RJ, Harper TW, Murphy RC, Austen KF.
Title
Identification and functional characterization of leukotriene B4 20-hydroxylase of human polymorphonuclear leukocytes.
Journal
Proc Natl Acad Sci U S A 82:2292-5 (1985)
DOI:
10.1073/pnas.82.8.2292
Other DBs
ExplorEnz - The Enzyme Database:
1.14.14.94
IUBMB Enzyme Nomenclature:
1.14.14.94
ExPASy - ENZYME nomenclature database:
1.14.14.94
BRENDA, the Enzyme Database:
1.14.14.94
CAS:
90119-11-2
LinkDB
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DBGET
integrated database retrieval system