KEGG   ENZYME: 2.3.2.16
Entry
EC 2.3.2.16                 Enzyme                                 

Name
lipid II:glycine glycyltransferase;
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:N6-glycine transferase;
femX (gene name);
alanyl-D-alanine-diphospho-ditrans,octacis-undecaprenyl-N-acetylglucosamine:glycine N6-glycyltransferase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
Sysname
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine N6-glycyltransferase
Reaction(IUBMB)
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + tRNAGly [RN:R08776]
Reaction(KEGG)
R08776
Substrate
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]
Product
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]
Comment
The enzyme from Staphylococcus aureus catalyses the transfer of glycine from a charged tRNA to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphosphoundecaprenyl-GlcNAc (lipid II), attaching it to the N6 of the L-Lys at position 3 of the pentapeptide. This is the first step in the synthesis of the pentaglycine interpeptide bridge that is used in S. aureus for the crosslinking of different glycan strands to each other. Four additional Gly residues are subsequently attached by EC 2.3.2.17 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase) and EC 2.3.2.18 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase).
History
EC 2.3.2.16 created 2010
Pathway
ec00550  Peptidoglycan biosynthesis
ec01100  Metabolic pathways
Orthology
K11693  peptidoglycan pentaglycine glycine transferase (the first glycine)
Genes
DAK: DaAHT2_1769
DALK: DSCA_12040
SDO: SD1155_00950
SAU: SA2057(fmhB)
SAV: SAV2262(fmhB)
SAW: SAHV_2246(fmhB)
SAH: SaurJH1_2329
SAJ: SaurJH9_2288
SAM: MW2180(fmhB)
SAS: SAS2152
SAR: SAR2346(fmhB)
SAC: SACOL2253(femX)
SAX: USA300HOU_2244(fmhB)
SAE: NWMN_2163(fmhB)
SAD: SAAV_2326(femX)
SUU: M013TW_2220(fmhB)
SUJ: SAA6159_02168(fmhB)
SUK: SAA6008_02299(fmhB)
SUQ: HMPREF0772_10937(femX)
SUZ: MS7_2282(femX)
SUX: SAEMRSA15_21600(fmhB)
SUW: SATW20_23950(fmhB)
SUG: SAPIG2314
SUF: SARLGA251_20470(fmhB)
SAUA: SAAG_00090
SAUE: RSAU_002097(femX)
SAUS: SA40_2008(fmhB)
SAUU: SA957_2092(fmhB)
SAUG: SA268_2163(fmhB)
SAUZ: SAZ172_2361(fmhB)
SAUV: SAI7S6_1016700(femX)
SAUW: SAI5S5_1016640(femX)
SAUX: SAI6T6_1016650(femX)
SAUY: SAI8T7_1016680(femX)
SAUF: X998_2246(fmhB)
SAB: SAB2134c(fmhB)
SUY: SA2981_2200(fmhB)
SAUB: C248_2299(fmhB)
SAUC: CA347_2342(femX)
SAUR: SABB_01408(femX)
SAUI: AZ30_11940
SAUD: CH52_07650
SAMS: NI36_11150
SER: SERP1844(femX)
SEP: SE1835
SEPP: SEB_01846
SEPS: DP17_786(femX)
SHA: SH0790(fmtB)
SHH: ShL2_00689(fmhB)
SSP: SSP0651
SCA: SCA_1750(fmhB)
SLN: SLUG_07600(fmhB)
SDT: SPSE_0559(femX)
SPAS: STP1_0738(femX)
SXO: SXYL_00693(femX)
SCAP: AYP1020_1437(femX)
SSCH: LH95_02915
SSCZ: RN70_03190
SAGQ: EP23_06995
SDP: NCTC12225_00638(femX)
SARL: SAP2_07240(femX)
SPIC: SAMEA4384060_0782(fmhB)
SSH: NCTC13712_02209(femX)
SSIM: SAMEA4384339_2091(fmhB)
MCL: MCCL_0176
MCAK: MCCS_02970(femX)
ESI: Exig_1829
EAN: Eab7_1680
PLV: ERIC2_c17540(femX)
AUR: HMPREF9243_0081(femX)
CPE: CPE2090
CPF: CPF_2347
CPR: CPR_2059
CLO: HMPREF0868_0028(femX)
CCEL: CCDG5_0870
SWO: Swol_2417
SLP: Slip_2315
SALQ: SYNTR_2105
DSY: DSY4943
DHD: Dhaf_4843
SGY: Sgly_3298
HMO: HM1_1069
TMR: Tmar_0525
MTA: Moth_2408
MTHO: MOTHE_c24880(femX)
MTHZ: MOTHA_c25600(femX)
TPZ: Tph_c27860(femX)
TOC: Toce_0288
FMA: FMG_0801
ACH: Achl_1256
CAG: Cagg_3053
TRO: trd_0929
ATM: ANT_22610
TPED: TPE_2360
SAAL: L336_0559
TMG: US01_C0001G0727(femAB)
BGW: VE98_C0001G0551(femX)
 » show all
Reference
1  [PMID:15228543]
  Authors
Schneider T, Senn MM, Berger-Bachi B, Tossi A, Sahl HG, Wiedemann I
  Title
In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.
  Journal
Mol Microbiol 53:675-85 (2004)
DOI:10.1111/j.1365-2958.2004.04149.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.16
IUBMB Enzyme Nomenclature: 2.3.2.16
ExPASy - ENZYME nomenclature database: 2.3.2.16
BRENDA, the Enzyme Database: 2.3.2.16
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