KEGG   ENZYME: 2.3.2.22
Entry
EC 2.3.2.22                 Enzyme                                 

Name
cyclo(L-leucyl-L-leucyl) synthase;
YvmC;
cLL synthase;
cyclodileucine synthase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
Sysname
L-leucyl-tRNALeu:L-leucyl-tRNALeu leucyltransferase (cyclizing)
Reaction(IUBMB)
2 L-leucyl-tRNALeu = 2 tRNALeu + cyclo(L-leucyl-L-leucyl) [RN:R10336]
Reaction(KEGG)
R10336
Substrate
L-leucyl-tRNA(Leu) [CPD:C02047]
Product
tRNA(Leu) [CPD:C01645];
cyclo(L-leucyl-L-leucyl) [CPD:C20514]
Comment
The reaction proceeds following a ping-pong mechanism forming a covalent intermediate between an active site serine and the first L-leucine residue [2]. The proteins from bacteria of the genus Bacillus also form small amounts of cyclo(L-phenylalanyl-L-leucyl) and cyclo(L-leucyl-L-methionyl) [1].
History
EC 2.3.2.22 created 2013
Orthology
K17485  cyclo(L-leucyl-L-leucyl) synthase
Genes
PLU: plu0297
PLUM: A4R40_01450
MME: Marme_0276
BSU: BSU35070(yvmC)
BSL: A7A1_1219
BSH: BSU6051_35070(yvmC)
BSUT: BSUB_03737
BSUL: BSUA_03737(yvmC)
BSUS: Q433_19195
BSQ: B657_35070
BSS: BSUW23_17190(yvmC)
BST: GYO_3844
BLI: BL00817(yvmC)
BLD: BLi03566(yvmC)
BLH: BaLi_c36280(yvmC)
BACY: QF06_15920
BACL: BS34A_38170(yvmC)
BALM: BsLM_3514
SEPS: DP17_2385
SHA: pSHaeC06
SDT: SPSE_0787
CJK: jk0923
 » show all
Reference
1  [PMID:19430487]
  Authors
Gondry M, Sauguet L, Belin P, Thai R, Amouroux R, Tellier C, Tuphile K, Jacquet M, Braud S, Courcon M, Masson C, Dubois S, Lautru S, Lecoq A, Hashimoto S, Genet R, Pernodet JL
  Title
Cyclodipeptide synthases are a family of tRNA-dependent peptide bond-forming enzymes.
  Journal
Nat Chem Biol 5:414-20 (2009)
DOI:10.1038/nchembio.175
  Sequence
[bsu:BSU35070] [plu:plu0297]
Reference
2  [PMID:21325056]
  Authors
Bonnefond L, Arai T, Sakaguchi Y, Suzuki T, Ishitani R, Nureki O
  Title
Structural basis for nonribosomal peptide synthesis by an aminoacyl-tRNA synthetase paralog.
  Journal
Proc Natl Acad Sci U S A 108:3912-7 (2011)
DOI:10.1073/pnas.1019480108
  Sequence
[bli:BL00817]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.22
IUBMB Enzyme Nomenclature: 2.3.2.22
ExPASy - ENZYME nomenclature database: 2.3.2.22
BRENDA, the Enzyme Database: 2.3.2.22
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