KEGG ENZYME: 2.5.1.136

ENZYME: 2.5.1.136

Entry

EC 2.5.1.136                Enzyme

Name

2-acylphloroglucinol 4-prenyltransferase;
PT-1 (gene name);
PT1L (gene name);
aromatic prenyltransferase (ambiguous);
dimethylallyl-diphosphate:2-acylphloroglucinol 4-dimethylallyltransferase

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

prenyl-diphosphate:2-acylphloroglucinol 4-prenyltransferase

Reaction(IUBMB)

prenyl diphosphate + a 2-acylphloroglucinol = diphosphate + a 2-acyl-4-prenylphloroglucinol [RN:R11609]

Reaction(KEGG)

R11609

Substrate

prenyl diphosphate [CPD:C00235];
2-acylphloroglucinol [CPD:C21498]

Product

diphosphate [CPD:C00013];
2-acyl-4-prenylphloroglucinol [CPD:C21499]

Comment

The enzyme, characterized from hop (Humulus lupulus), acts on phlorisovalerophenone, phlormethylbutanophenone, and phlorisobutanophenone during the synthesis of bitter acids. It also acts with much lower activity on naringenin chalcone. Forms a complex with EC 2.5.1.137, 2-acyl-4-prenylphloroglucinol 6-prenyltransferase, which catalyses additional prenylation reactions. Requires Mg2+.

History

EC 2.5.1.136 created 2017

Orthology

K21590

2-acylphloroglucinol 4-prenyltransferase

Reference

1 [PMID:22166201]

Authors

Tsurumaru Y, Sasaki K, Miyawaki T, Uto Y, Momma T, Umemoto N, Momose M, Yazaki K

Title

HlPT-1, a membrane-bound prenyltransferase responsible for the biosynthesis of bitter acids in hops.

Journal