KEGG ENZYME: 2.5.1.149

ENZYME: 2.5.1.149

Entry

EC 2.5.1.149                Enzyme

Name

lycopene elongase/hydratase (flavuxanthin-forming);
crtEb (gene name);
dimethylallyl-diphosphate:all-trans-lycopene dimethylallyltransferase (hydrating, flavuxanthin-forming)

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

prenyl-diphosphate:all-trans-lycopene prenyltransferase (hydrating, flavuxanthin-forming)

Reaction(IUBMB)

(1) prenyl diphosphate + all-trans-lycopene + acceptor + H2O = nonaflavuxanthin + reduced electron acceptor + diphosphate [RN:R12283];
(2) prenyl diphosphate + nonaflavuxanthin + acceptor + H2O = flavuxanthin + reduced electron acceptor + diphosphate [RN:R12284]

Reaction(KEGG)

R12283 R12284

Substrate

prenyl diphosphate [CPD:C00235];
all-trans-lycopene [CPD:C05432];
acceptor [CPD:C00028];
H2O [CPD:C00001];
nonaflavuxanthin [CPD:C22056]

Product

nonaflavuxanthin [CPD:C22056];
reduced electron acceptor;
diphosphate [CPD:C00013];
flavuxanthin [CPD:C22057]

Comment

The enzyme, characterized from the bacterium Corynebacterium glutamicum, is bifunctional. It catalyses the elongation of the C40 carotenoid all-trans-lycopene by attaching an isoprene unit at C-2, as well as the hydroxylation of the new isoprene unit. The enzyme acts at both ends of the substrate, forming the C50 carotenoid flavuxanthin via the C45 intermediate nonaflavuxanthin. cf. EC 2.5.1.150, lycopene elongase/hydratase (dihydrobisanhydrobacterioruberin-forming).

History

EC 2.5.1.149 created 2018

Orthology

K23155

lycopene elongase/hydratase (flavuxanthin-forming)

Genes

RUV:

EC9_18980

PND:	Pla175_07110

UAM:	UABAM_00068

UHL:	UABHE_001783

AIC:	JK629_03455

CGL:	Cgl0620(Cgl0620)

CGB:	cg0717(crtEb)

CGU:	WA5_0594(CrtEb)

CGT:

cgR_0740

CGS:	C624_03875(ubiA)

CGG:	C629_03875(ubiA)

CGM:	cgp_0717(crtEb)

CGJ:	AR0_03700(ubiA)

CGQ:	CGLAR1_03560(ubiA)

CGX:	SB89_03315(ubiA)

CEF:	CE0637(crtEb)

CHN:	A605_03535(ubiA)

CCN:	H924_00345(ubiA)

CMD:	B841_05595(ubiA)

CFN:	CFAL_10280(ubiA)

CVT:	B843_10365(ubiA)

CUV:	CUREI_00105(ubiA)

CHM:	B842_00595(ubiA)

CMQ:	B840_00375(ubiA)

CMV:	CMUST_03130(ubiA)

CTED:

CTEST_03075

CDX:	CDES_03195(ubiA)

CSTA:

CSTAT_10575(ubiA)

CCJZ:

ccrud_03140(ubiA)

CFC:	CFLV_10475(ubiA)

CPEG:

CPELA_00975

CEE:	CENDO_09950

CSAN:

E3227_00065

CGK:	CGERO_00975

CRL:	NCTC7448_00519(ubiA)

CCHO:

CCHOA_00110

CPSO:

CPPEL_00910

CSUR:

N24_0758(ubiA)

CCYS:

SAMEA4530656_2401(ubiA)

CYZ:	C3B44_06280

CUO:	CUROG_08910

CQN:	G7Y29_03570

CCOE:

CETAM_00640

COK:	COCCU_07355

CWK:	IA203_00750

CFG:	CFREI_03330

CFAC:

CFAEC_02680

CATR:

CATRI_02910 CATRI_10290

CHAN:

CHAN_00205

CGF:	CGUA_00070

CAPP:

CAPP_09505

CSUE:

QP029_06860

CORE:

RIU96_06425

BFV:	C628_03535(ubiA)

SNK:	CP967_33620

NAEI:

GCM126_12280

ACAO:

NF551_13220

AZH:	MUK71_13160

AGEG:

MUG94_14205

AKO:	N9A08_12980

AZX:	N2K95_13100

AJR:	N2K98_13805

ARTL:

ArtHe_11080

PDEL:

JCQ34_14720

» show all

Reference

1 [PMID:11432736]

Authors

Krubasik P, Kobayashi M, Sandmann G

Title

Expression and functional analysis of a gene cluster involved in the synthesis of decaprenoxanthin reveals the mechanisms for C50 carotenoid formation.

Journal

Eur J Biochem 268:3702-8 (2001)
DOI:10.1046/j.1432-1327.2001.02275.x

Sequence

[cgb:cg0717]

Reference

2 [PMID:22963379]

Authors

Heider SA, Peters-Wendisch P, Wendisch VF

Title

Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum.

Journal

BMC Microbiol 12:198 (2012)
DOI:10.1186/1471-2180-12-198