KEGG ENZYME: 2.5.1.97

ENZYME: 2.5.1.97

Entry

EC 2.5.1.97                 Enzyme

Name

pseudaminic acid synthase;
PseI;
NeuB3

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

phosphoenolpyruvate:2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose transferase (phosphate-hydrolysing, 2,7-acetylamino-transfering, 2-carboxy-2-oxoethyl-forming)

Reaction(IUBMB)

phosphoenolpyruvate + 2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose + H2O = 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid + phosphate [RN:R09841]

Reaction(KEGG)

R09841

Substrate

phosphoenolpyruvate [CPD:C00074];
2,4-bis(acetylamino)-2,4,6-trideoxy-beta-L-altropyranose;
H2O [CPD:C00001]

Product

5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid [CPD:C20082];
phosphate [CPD:C00009]

Comment

The enzyme requires a divalent metal ion, the highest activity values are observed in the presence of Mn2+ and Co2+ (10 mM).

History

EC 2.5.1.97 created 2011

Pathway

ec00541

Biosynthesis of various nucleotide sugars

ec01100

Metabolic pathways

Orthology

K15898

pseudaminic acid synthase

Genes

SHM:	Shewmr7_1391

SJA:	SJ2017_2979

IAB:	K5X84_03460(pseI)

AMG:	AMEC673_04890

FPZ:	LA55_1639(pseI)

FPER:

ACH24_00845

FAD:	CDH04_02740(pseI)

FMI:	F0R74_04805(pseI)

FGU:	SD28_06730

TLH:	NR989_03670(pseI)

ACAV:

VI35_07445(pseI)

AEA:	C2U39_08085(pseI)

ARV:	C7N77_17160(pseI)

ASIM:

FE240_05740(pseI)

PAGI:

ABHF91_11850(pseI)

PSE:

NH8B_3550

AMAH:

DLM_3379

CHIC:

N8I74_00610(pseI)

BVIT:

JIP62_12975(pseI)

BREA:

HZ989_05585(pseI)

HPY:

HP_0178

HEO:	C694_00885

HPJ:	jhp_0166(neuB)

HPA:	HPAG1_0175

HPS:	HPSH_00900

HHP:	HPSH112_00870

HHQ:	HPSH169_00865

HHR:	HPSH417_00875

HPG:

HPG27_163

HPP:	HPP12_0178

HPB:	HELPY_0182(neuB)

HPL:	HPB8_1388(neuB)

HPC:	HPPC_00900

HCA:	HPPC18_00890

HPM:	HPSJM_00980

HPE:	HPELS_05885

HPO:	HMPREF4655_20420(pseI)

HPI:	hp908_0187

HPQ:	hp2017_0183(neuB)

HPW:	hp2018_0186

HPU:	HPCU_00850

HEF:	HPF16_0186(neuB)

HPF:	HPF30_1116(neuB)

HEQ:	HPF32_0188(neuB)

HEX:	HPF57_0197(neuB)

HPT:	HPSAT_00865

HPZ:

HPKB_0188

HPV:	HPV225_0194

HPX:	HMPREF0462_0233(pseI)

HEN:	HPSNT_01075

HPH:	HPLT_00935

HEG:	HPGAM_00920

HPN:	HPIN_06250

HEP:	HPPN120_00905

HEU:	HPPN135_00890

HES:	HPSA_00905

HPYS:

HPSA20_0199(pseI)

HCN:	HPB14_00860

HPD:	KHP_0177(neuB)

HEY:

MWE_0252

HER:	C695_00885

HEI:	C730_00885

HPYA:

HPAKL117_00890

HPYK:

HPAKL86_06095

HPYO:

HPOK113_0186(neuB)

HPYL:

HPOK310_0184(neuB)

HPYB:

HPOKI102_01200

HPYC:

HPOKI112_01195

HPYD:

HPOKI128_00980

HPYE:

HPOKI154_00970

HPYF:

HPOKI422_01195

HPYG:

HPOKI673_00975

HPYH:

HPOKI828_00965

HPYJ:

HPOKI898_01205

HPYR:

K747_09550

HPYI:

K750_07650

HPYU:

K751_06745

HPYM:

K749_04795

HEM:	K748_03215

HEB:

U063_0520

HEZ:

U064_0521

HHE:

HH_0908

HAC:

Hac_0364

HMS:	HMU11700(neuB3)

HFE:	HFELIS_15210(neuB3)

HBI:	HBZC1_10970

HCE:

HCW_03725

HCM:

HCD_03040

HCP:

HCN_1783

HCB:	HCBAA847_2066

HHM:	BN341_3140

HTY:	BN2458_PEG0381

HBL:	XJ32_01420

HAD:	CDV25_07195(pseI)

HET:	BBW65_06570

HCL:	NCTC13205_00368(spsE_1)

HWI:	A0Z60_02455(pseI)

HSH:	NHP194022_15540(neuB)

HPUL:

NCTC13154_00281(spsE)

HAIL:

ASB7_09380(pseI)

HFI:	NHP21005_02350(neuB)

HGA:	NHP190012_07660(neuB)

HGS:	NHP190003_09930(neuB)

HMY:	V3I05_06960(pseI)

WSU:

WS2109

SUA:

Saut_0136

SKU:	Sulku_0155

SULR:

B649_00690

CJE:	Cj1317(pseI)

CJB:	BN148_1317(pseI)

CJJ:	CJJ81176_1334(neuB)

CJU:	C8J_1251(neuB)

CJN:	ICDCCJ07001_1263

CJI:	CJSA_1255(pseI)

CJM:	CJM1_1290(pseI)

CJS:

CJS3_1417

CJP:	A911_06405

CJEJ:

N564_01335

CJEU:

N565_01374

CJEN:

N755_01370

CJEI:

N135_01406

CJER:

H730_07535

CJV:	MTVDSCj20_1313(pseI)

CJY:	QZ67_01452(pseI)

CJQ:	UC78_1266(pseI)

CJL:	PJ17_06975

CJW:	PJ18_06780

CJR:	CJE1512(neuB)

CJD:	JJD26997_0397

CJZ:	M635_02275

CJX:	BN867_13060

CFF:	CFF8240_1556

CFT:	CFF04554_1565(pseI)

CFV:	CFVI03293_1587(pseI)

CFX:	CFV97608_1689(pseI)

CFZ:

CSG_16920

CAMP:

CFT03427_1513(pseI)

CFP:	CR44_07675

CCV:	CCV52592_0565(pseI)

CLA:	CLA_0169(pseI)

CLR:	UPTC16701_0178(pseI)

CLM:	UPTC16712_0176(pseI)

CLQ:	UPTC4110_0170(pseI)

CLN:	UPTC3659_0189(pseI)

CLL:	CONCH_0164(pseI)

CCOL:

BN865_08190c

CCC:	G157_02210

CCQ:	N149_1280(pseI)

CCF:

YSQ_02260

CCY:

YSS_07155

CCOI:

YSU_02275

CCOF:

VC76_06635(pseI)

CCOO:

ATE51_00930(pseI)

CAJ:	CIG1485E_1505(pseI)

CIS:	CINS_0157(pseI)

CVO:	CVOL_0160(pseI)

CPEL:

CPEL_0174(pseI)

CAMR:

CAQ16704_0169(pseI)

CSM:	CSUB8521_0179(pseI)

CSF:	CSUB8523_0173(pseI)

CHYO:

CHH_1600(pseI)

CHV:	CHELV3228_0385(pseI)

CPIN:

CPIN18020_0127(pseI)

CCUN:

CCUN_1340(pseI)

CLX:	CLAN_1240(pseI)

CAVI:

CAV_1218(pseI)

CHW:	A2J15_000480(pseI)

CAMZ:

CVIC12175_0323(pseI)

CAMY:

CSUIS_1327(pseI)

COJ:	CORN_0174(pseI)

CUX:	CUP3940_1257(pseI)

CRX:	CRECT_0228(pseI)

CARM:

CARM_0135(pseI)

CMUC:

CMCT_1057(pseI)

CSHO:

CSHOW_1837(pseI)

CINF:

CINF_1035(pseI)

CNV:	CNZW441b_1300(pseI)

CVU:	CVULP_0373(pseI)

CDEV:

CIGN_1364(pseI)

CCAQ:

CCAL_1596(pseI)

CMOL:

CMOL_0978(pseI)

SDL:

Sdel_2213

SBA:	Sulba_2447

SMUL:

SMUL_3176(pseI)

SHAL:

SHALO_2912

SULS:

Sdiek1_2901

SULJ:

SJPD1_2797

ABU:	Abu_2246(neuB)

ABT:

ABED_2056

ABL:	A7H1H_2193(pseI)

ACRE:

ACRYA_0127(pseI)

ALAN:

ALANTH_2129(pseI)

AFC:	AFAEC_0081(pseI)

ATHR:

ATH_1859(pseI)

ANT:	Arnit_2950

AAQI:

AAQM_2371(pseI)

AVP:	AVENP_2942(pseI)

APOC:

APORC_1981(pseI)

AROS:

NJU99_14105(pseI)

AIE:	LXN10_05630(pseI)

AMAR:

AMRN_2660(pseI)

ACAA:

ACAN_2663(pseI)

AMOL:

AMOL_2612(pseI)

HYO:

NNO_0332

HTM:	RZR91_12640(pseI)

SINU:

IMZ28_03850(pseI)

NAM:

NAMH_1595

NAP:	C3L23_08285(pseI)

CMED:

FE773_01415(pseI)

CPAF:

C6V80_09010(pseI)

HMR:	Hipma_1649

DSF:

UWK_02421

TID:	Thein_0449

BFG:	BF638R_3762

BHL:	Bache_1948

BHF:	C3V43_13585(pseI)

BEG:	INE88_04056(pseI)

BHUM:

JXR92_011985(pseI)

BSAL:

K6V25_09565(pseI)

BACE:

H1A11_05720(pseI)

PET:	PEIBARAKI_4457

PSAC:

PSM36_1392(pseI)

PARC:

CI960_11570(pseI)

OSP:	Odosp_0125

PGS:	CPT03_16765(pseI)

PEDO:

AB3G38_15150(pseI)

SPHN:

BV902_02100

SPDR:

G6053_00975(pseI)

SSIY:

JVX97_02420(pseI)

EST:	DN752_19920(pseI)

PSEZ:

HME7025_02498(pseI)

AANH:

G9X63_04255(pseI)

DYC:	NFI80_14160(pseI)

DFQ:	NFI81_15315(pseI)

DCN:	MUK70_18795(pseI)

PACT:

CA264_07975

MSAA:

QYS49_02435(pseI)

FLM:	MY04_2866(pseI)

FUA:	LVD17_18025(pseI)

CNQ:	PZB72_13120(pseI)

ATK:	AUTU_44530

GFO:	GFO_2016(NeuB)

FIN:	KQS_04780(neuB)

FFA:	FFWV33_16665

FPAL:

HYN49_00315(pseI)

FMG:	HYN48_00290(pseI)

FFL:	HYN86_01685(pseI)

FOK:	KK2020170_15430

FCG:	LNP23_01695(pseI)

FLAV:

HAV12_004185(pseI)

CAT:	CA2559_13043

DOD:	DCS32_13465(pseI)

ZLA:	Q5W13_08655(pseI)

NOM:	AAT17_01915

NUL:	R1T42_06075(pseI)

PHAL:

H9I45_06435(pseI)

PLIT:

K8354_12850(pseI)

PAAE:

NPX36_13245(pseI)

WIN:

WPG_0822

WIJ:	BWZ20_09290

TMAR:

MARIT_1555(pseI)

TPAC:

PG913_04905(pseI)

FOR:	FORMB_12950

FOH:	FORMA_12200

TAY:	JJL45_03330(pseI)

TAJ:	C1A40_06900(pseI)

MLIL:

QLS71_006835(pseI)

ANP:	FK178_01775(pseI)

BIA:	GMA17_12820(pseI)

FBM:	MQE35_08980(pseI)

SAHN:

JRG66_01025(pseI)

SAZS:

VS868_14080(pseI)

FBU:	UJ101_00315(neuB)

FTE:	Fluta_0224

FLU:	CHH17_13955(pseI)

CRM:	K6119_04505(pseI)

MRO:

MROS_1160

HTH:	HTH_1538(neuB)

HTE:	Hydth_1526

DAP:	Dacet_0631

GTL:	EP073_12670(pseI)

CNI:	Calni_0723

MSCH:

N508_001046(pseI_1) N508_001414(pseI_2)

BSHI:

LGQ02_19515(pseI)

GCS:	MUN88_20020(pseI)

EXM:	U719_13415

JEO:

JMA_28170

AVS:	AWM76_07140

JEP:	BW721_08310

CAC:	CA_C2187(spsE)

CAE:

SMB_G2220

CAY:	CEA_G2201(spsE)

CTET:

BN906_01852

CNO:	NT01CX_1891

CBO:

CBO2720

CBA:

CLB_2661

CBH:

CLC_2594

CBY:	CLM_3086(pseI)

CBL:

CLK_2105

CBK:

CLL_A0832

CBI:

CLJ_B2947

CBT:

CLH_0798

CBJ:	H04402_02802

CBE:

Cbei_4278

CBZ:

Cbs_4278

CBEI:

LF65_04801

CLJ:	CLJU_c05510

CSR:	Cspa_c45320(pseI)

CSB:	CLSA_c39500(pseI)

CAH:	CAETHG_2633(pseI)

CLT:	CM240_1654(pseI)

CSQ:

CSCA_3176

CLD:	CLSPO_c27820(pseI)

CEU:	A7L45_14325

CTAE:

BGI42_03935(pseI)

CFM:	BJL90_07025

CARG:

RSJ17_12835

CDRK:

B9W14_04045

CDY:	F3K33_21895(pseI)

CCOH:

SAMEA4530647_1491(spsE)

CFER:

D4Z93_09240(pseI)

CLOS:

DMR38_02440(pseI)

CRW:	CROST_020420(pseI)

CFB:	CLFE_020370(pseI)

CAUN:

CLAUR_009480(pseI)

CNN:	CNEO_3840(pseI)

CINT:

HZF06_22520(pseI)

OCW:	OW730_17355(pseI) OW730_21380(pseI)

CTH:

Cthe_2229

CTX:	Clo1313_2902

CCL:	Clocl_4082

HSC:	HVS_01475(pseI1) HVS_04485(pseI2)

RHER:

EHE19_000755(pseI)

DOR:	Desor_4905

ACAE:

HYG86_00790(pseI)

BPEN:

NQ488_12705(pseI)

BPB:	bpr_I2559(neuB2)

BHU:

bhn_I2423

RHO:	RHOM_00785

AHB:	bsdtb5_38070

ERE:	EUBREC_3247

KIB:	RBB56_12260(pseI)

KSI:	V6984_18860(pseI)

CBIL:

EUBC25_01560

LBP:	V1224_13590(pseI)

AOE:

Clos_2510

GFE:	Gferi_06945

CSPO:

QNI18_06135(pseI)

PBIF:

KXZ80_05435(pseI)

TEM:	JW646_12045(pseI)

TGC:	L0P85_15665(pseI)

TPET:

TPHSE_34860(pseI)

TMT:	Tmath_0718

KME:	H0A61_02803(pseI)

KPAR:

JL105_08005(pseI)

CAZO:

G3A45_09455(pseI)

SRI:	SELR_23720

SOVA:

SOV_39710(pseI)

FBS:	N510_001652(pseI)

ELE:

Elen_2425

EYY:	EGYY_09060

MAEO:

JRV97_05815(pseI)

FCI:	I6I83_02445(pseI)

HABY:

HLVA_17210

BBGW:

UT28_C0001G0399

NBN:	HYY55_00155(pseI)

MSI:

Msm_1539

METV:

K4897_08545(pseI)

MCUB:

MCBB_1285(pseI)

MFEG:

GCM10025860_24300

MEQ:	MSWHS_1969

MPY:

Mpsy_2359

PSYT:

DSAG12_01856(pseI)

» show all

Reference

1 [PMID:16120604]

Authors

Chou WK, Dick S, Wakarchuk WW, Tanner ME

Title

Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase.

Journal

J Biol Chem 280:35922-8 (2005)
DOI:10.1074/jbc.M507483200

Sequence

[cje:Cj1317]

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.97

IUBMB Enzyme Nomenclature:

2.5.1.97

ExPASy - ENZYME nomenclature database:

2.5.1.97

BRENDA, the Enzyme Database:

2.5.1.97

LinkDB

DBGET integrated database retrieval system