KEGG   ENZYME: 3.2.1.113
Entry
EC 3.2.1.113                Enzyme                                 

Name
mannosyl-oligosaccharide 1,2-alpha-mannosidase;
mannosidase 1A;
mannosidase 1B;
1,2-alpha-mannosidase;
exo-alpha-1,2-mannanase;
mannose-9 processing alpha-mannosidase;
glycoprotein processing mannosidase I;
mannosidase I;
Man9-mannosidase;
ManI;
1,2-alpha-mannosyl-oligosaccharide alpha-D-mannohydrolase;
MAN1A1 (gene name);
MAN1A2 (gene name);
MAN1C1 (gene name);
2-alpha-mannosyl-oligosaccharide alpha-D-mannohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
Man9GlcNAc2-[protein] alpha-2-mannohydrolase (configuration-inverting)
Reaction(IUBMB)
(1) Man9GlcNAc2-[protein] + 4 H2O = Man5GlcNAc2-[protein] + 4 beta-D-mannopyranose (overall reaction);
(1a) Man9GlcNAc2-[protein] + H2O = Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2) + beta-D-mannopyranose;
(1b) Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2) + H2O = Man7GlcNAc2-[protein] (isomer 7A1,2,3B2) + beta-D-mannopyranose;
(1c) Man7GlcNAc2-[protein] (isomer 7A1,2,3B2) + H2O = Man6GlcNAc2-[protein] (isomer 6A1,2B2) + beta-D-mannopyranose;
(1d) Man6GlcNAc2-[protein] (isomer 6A1,2B2) + H2O = Man5GlcNAc2-[protein] + beta-D-mannopyranose;
(2) Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + 3 H2O = Man5GlcNAc2-[protein] + 3 beta-D-mannopyranose (overall reaction);
(2a) Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3) + H2O = Man7GlcNAc2-[protein] (isomer 7A1,2,3B1) + beta-D-mannopyranose;
(2b) Man7GlcNAc2-[protein] (isomer 7A1,2,3B1) + H2O = Man6GlcNAc2-[protein] (isomer 6A1,2,3) + beta-D-mannopyranose;
(2c) Man6GlcNAc2-[protein] (isomer 6A1,2,3) + H2O = Man5GlcNAc2-[protein] + beta-D-mannopyranose
Reaction(KEGG)
(other) R12479(G)
Substrate
Man9GlcNAc2-[protein];
H2O [CPD:C00001];
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2);
Man7GlcNAc2-[protein] (isomer 7A1,2,3B2);
Man6GlcNAc2-[protein] (isomer 6A1,2B2);
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,3);
Man7GlcNAc2-[protein] (isomer 7A1,2,3B1);
Man6GlcNAc2-[protein] (isomer 6A1,2,3)
Product
Man5GlcNAc2-[protein];
beta-D-mannopyranose;
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2);
Man7GlcNAc2-[protein] (isomer 7A1,2,3B2);
Man6GlcNAc2-[protein] (isomer 6A1,2B2);
Man7GlcNAc2-[protein] (isomer 7A1,2,3B1);
Man6GlcNAc2-[protein] (isomer 6A1,2,3)
Comment
This family of mammalian enzymes, located in the Golgi system, participates in the maturation process of N-glycans that leads to formation of hybrid and complex structures. The enzymes catalyse the hydrolysis of the four (1->2)-linked alpha-D-mannose residues from the Man9GlcNAc2 oligosaccharide attached to target proteins as described in reaction (1). Alternatively, the enzymes act on the Man8GlcNAc2 isomer formed by EC 3.2.1.209, endoplasmic reticulum Man9GlcNAc2 1,2-alpha-mannosidase, as described in reaction (2). The enzymes are type II membrane proteins, require Ca2+, and use an inverting mechanism. While all three human enzymes can catalyse the reactions listed here, some of the enzymes can additionally catalyse hydrolysis in an alternative order, generating additional isomeric intermediates, although the final product is the same. The names of the isomers listed here are based on a nomenclature system proposed by Prien et al [7].
History
EC 3.2.1.113 created 1986, modified 2019
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K01230  mannosyl-oligosaccharide alpha-1,2-mannosidase
Genes
HSA: 10905(MAN1A2) 4121(MAN1A1) 57134(MAN1C1)
PTR: 457159(MAN1A2) 463148(MAN1A1) 736491(MAN1C1)
PPS: 100977047(MAN1A2) 100983605(MAN1A1) 100984948(MAN1C1)
GGO: 101133209(MAN1A2) 101142550(MAN1C1) 101144345(MAN1A1)
PON: 100434522(MAN1A1) 100439526(MAN1C1) 100459953(MAN1A2)
NLE: 100579310(MAN1A1) 100592662(MAN1A2) 100594141(MAN1C1)
MCC: 711548(MAN1A2) 714609(MAN1A1) 719686(MAN1C1)
MCF: 102116178(MAN1A2) 102133475(MAN1C1) 102134558(MAN1A1)
CSAB: 103224125(MAN1A2) 103225352(MAN1C1) 103240478(MAN1A1)
RRO: 104656091(MAN1C1) 104670284(MAN1A1) 104670672(MAN1A2)
RBB: 108518016(MAN1C1) 108528145(MAN1A2) 108529738(MAN1A1)
CJC: 100387951(MAN1A1) 100396923(MAN1A2) 100411429(MAN1C1)
SBQ: 101029383(MAN1C1) 101039212(MAN1A1) 101047170(MAN1A2)
MMU: 17155(Man1a) 17156(Man1a2) 230815(Man1c1)
MCAL: 110290797(Man1a2) 110292777(Man1c1) 110302882(Man1a1)
MPAH: 110319841(Man1a2) 110323014(Man1c1) 110326269(Man1a1)
RNO: 294410(Man1a1) 295319(Man1a2) 362625(Man1c1)
MUN: 110539790(Man1a2) 110552127(Man1c1) 110563941(Man1a1) 110566577
CGE: 100761494(Man1c1) 100767553(Man1a1) 100770527(Man1a2)
NGI: 103725065(Man1c1) 103734033(Man1a2) 103736289(Man1a1)
HGL: 101699709(Man1c1) 101700934(Man1a2) 101714110(Man1a1)
CCAN: 109683046(Man1c1) 109685361 109685366 109693393(Man1a1)
OCU: 100009182(MAN1A1) 100338665(MAN1A2) 100354369(MAN1C1)
TUP: 102475708(MAN1A1) 102490275(MAN1A2) 102498287(MAN1C1)
CFA: 476275(MAN1A1) 478178(MAN1C1) 483141(MAN1A2)
VVP: 112916781(MAN1A2) 112922685(MAN1A1) 112931689(MAN1C1)
AML: 100467154 100478751(MAN1A2) 100480907(MAN1C1)
UMR: 103663450(MAN1A1) 103666782(MAN1C1) 103680866(MAN1A2)
UAH: 113244370(MAN1A2) 113259398(MAN1A1) 113268483(MAN1C1)
ORO: 101364359(MAN1A2) 101378145(MAN1A1) 101382672(MAN1C1)
FCA: 101086830(MAN1A2) 101099737(MAN1C1) 101100723(MAN1A1)
PTG: 102953645(MAN1A2) 102953958(MAN1A1) 102956478(MAN1C1)
PPAD: 109248477(MAN1A1) 109272149(MAN1A2) 109274338(MAN1C1)
AJU: 106968317(MAN1A1) 106977625(MAN1C1) 106978925(MAN1A2)
BTA: 530027(MAN1A1) 532645(MAN1A2) 615012(MAN1C1)
BOM: 102266017(MAN1A2) 102269574(MAN1A1) 102286189(MAN1C1)
BIU: 109555865 109563775(MAN1A1) 109563776 109576096(MAN1C1)
BBUB: 102390945(MAN1C1) 102403875(MAN1A1) 102411005(MAN1A2)
CHX: 102172355(MAN1A1) 102178963(MAN1A2) 102182552(MAN1C1)
OAS: 101103326(MAN1A1) 101108177(MAN1A2) 101118089(MAN1C1)
SSC: 100154147(MAN1A2) 100524150(MAN1C1) 396919(MAN1A1)
CFR: 102503605(MAN1A1) 102510529(MAN1A2) 102523440(MAN1C1)
CDK: 105088351(MAN1A1) 105089605(MAN1A2) 105100665(MAN1C1)
BACU: 103005884(MAN1A1) 103007272(MAN1C1) 103018894(MAN1A2)
LVE: 103070296(MAN1C1) 103087206(MAN1A2) 103087551(MAN1A1)
OOR: 101277960(MAN1A1) 101282517(MAN1A2) 101283566(MAN1C1)
DLE: 111163346(MAN1C1) 111165103(MAN1A1) 111179970(MAN1A2)
PCAD: 102982982(MAN1A1) 102991760(MAN1C1) 102996939(MAN1A2)
ECB: 100057488(MAN1C1) 100066466(MAN1A2) 100072869(MAN1A1)
EPZ: 103555530(MAN1A1) 103556781(MAN1C1) 103559230 103560293(MAN1A2)
EAI: 106830488(MAN1A1) 106835703(MAN1A2) 106839943(MAN1C1)
MYB: 102238828(MAN1A2) 102246069(MAN1A1) 102247391(MAN1C1)
MYD: 102763868(MAN1C1) 102764904(MAN1A1) 102766164(MAN1A2)
MNA: 107526302(MAN1A1) 107539790(MAN1C1) 107545314(MAN1A2)
DRO: 112297042(MAN1A1) 112299230(MAN1C1) 112314012(MAN1A2)
PALE: 102887313(MAN1A1) 102889844(MAN1A2) 102897246(MAN1C1)
RAY: 107502524(MAN1C1) 107515226(MAN1A1) 107518829(MAN1A2)
MJV: 108398774(MAN1C1) 108401964(MAN1A2) 108404531(MAN1A1)
LAV: 100657059(MAN1A2) 100658532(MAN1A1) 100671581(MAN1C1)
MDO: 100010854(MAN1A2) 100023598(MAN1A1) 100617488(MAN1C1)
SHR: 100913792(MAN1A1) 100925784(MAN1C1) 100934193(MAN1A2)
PCW: 110197597(MAN1A1) 110206094(MAN1C1) 110219515(MAN1A2)
OAA: 100079674(MAN1A1) 100081124(MAN1A2) 100082150(MAN1C1)
GGA: 418265(MAN1A2) 419588(MAN1C1) 421726(MAN1A1)
MGP: 100546016(MAN1A2) 100549489(MAN1C1) 100549765(MAN1A1)
CJO: 107311841(MAN1A1) 107319054(MAN1A2) 107323893(MAN1C1)
NMEL: 110387421(MAN1C1) 110395643(MAN1A1) 110398840(MAN1A2)
APLA: 101795067(MAN1A1) 101803658(MAN1A2)
ACYG: 106032458(MAN1A1) 106038021(MAN1C1) 106049769(MAN1A2)
TGU: 100220181(MAN1A2) 100221810(MAN1C1) 100232070(MAN1A1)
LSR: 110472867(MAN1C1) 110479685(MAN1A1) 110480834(MAN1A2)
SCAN: 103818314(MAN1A1) 103823519(MAN1C1) 103827137(MAN1A2)
GFR: 102038714(MAN1A1) 102039589(MAN1A2) 102039998(MAN1C1)
FAB: 101814086(MAN1A1) 101817258(MAN1A2) 101820958(MAN1C1)
PHI: 102101333(MAN1A2) 102108536(MAN1C1) 102109517(MAN1A1)
PMAJ: 107201780(MAN1A1) 107208361(MAN1A2) 107214221(MAN1C1)
CCAE: 111927193(MAN1A1) 111938572(MAN1A2) 111939097(MAN1C1)
CCW: 104687107(MAN1A2) 104691020(MAN1A1) 104694641(MAN1C1)
ETL: 114058197(MAN1A2) 114066157(MAN1A1) 114067076 114069020(MAN1C1)
FPG: 101910863(MAN1A1) 101918152(MAN1C1) 101922245
FCH: 102048637(MAN1C1) 102052529(MAN1A2) 102054649(MAN1A1)
CLV: 102087646(MAN1A2) 102093752(MAN1A1) 102094812(MAN1C1)
EGZ: 104126519(MAN1A2) 104131968(MAN1A1) 104134894(MAN1C1)
NNI: 104008647(MAN1A2) 104008861(MAN1A1) 104017726(MAN1C1)
ACUN: 113478539(MAN1A1) 113486782(MAN1A2) 113488235(MAN1C1)
PADL: 103918380(MAN1A1) 103921210(MAN1A2) 103923712(MAN1C1)
AAM: 106485019(MAN1C1) 106497207(MAN1A2) 106498000(MAN1A1)
ASN: 102369572(MAN1A1) 102378356(MAN1C1) 102388362(MAN1A2)
AMJ: 102558211(MAN1C1) 102563847(MAN1A1) 106736681(MAN1A2)
PSS: 102449928(MAN1A1) 102451974(MAN1C1) 102453471(MAN1A2)
CMY: 102930970(MAN1C1) 102934433 102944110(MAN1A1)
CPIC: 101932451(MAN1C1) 101948035(MAN1A2) 101950812(MAN1A1)
ACS: 100552898(man1a1) 100553205(man1c1) 100561704(man1a2)
PVT: 110076944(MAN1A2) 110083601(MAN1A1) 110084141(MAN1C1)
PBI: 103051064(MAN1C1) 103057418(MAN1A1) 103063461(MAN1A2)
PMUR: 107285786(MAN1A2) 107293110 107295591(MAN1C1) 107299749(MAN1A1)
TSR: 106539697(MAN1C1) 106542702 106544534(MAN1A1) 106550491
PMUA: 114587319(MAN1A2) 114594095(MAN1A1) 114601556(MAN1C1)
GJA: 107108705(MAN1A1) 107110812(MAN1C1) 107114859(MAN1A2)
XLA: 108708264 108716798 108717911 431910(man1c1.S) 446337(man1a2.L) 734993
XTR: 100216029(man1a2) 100485889(man1c1) 100491314(man1a1)
NPR: 108788290(MAN1C1) 108792840(MAN1A1) 108797861(MAN1A2)
DRE: 101882461 335538(man1a2) 566078(man1a1)
IPU: 108256527(man1c1) 108257868(man1a1) 108266693(man1a2)
PHYP: 113525570(man1a2) 113531863(man1a1) 113532696
AMEX: 103028203 103030688(man1a2) 103042818(man1a1)
EEE: 113572236(man1a1) 113580013 113587749(man1a2)
LCO: 104921495(man1a2) 104924713(man1c1) 104933631(man1a1)
MZE: 101464129 101465263(man1a1) 101483462(man1a2)
ONL: 100703754(man1a1) 100705345 109194341(man1a2)
OLA: 101155716(man1c1) 101158740 101162719(man1a2)
XMA: 102228830(man1a2) 102229946 102233097(man1a1)
XCO: 114141878(man1c1) 114148310(man1a2) 114158682(man1a1)
PRET: 103457245(man1a1) 103477781 103477910(man1a2)
KMR: 108240356 108244885(man1a1) 108247704(man1a2)
ALIM: 106510981 106523059(man1c1) 106530909(man1a2) 106531563
CSEM: 103381156(man1a1) 103387925 103391752(man1a2)
LCF: 108872812(man1a2) 108885258 108899472(man1c1)
SDU: 111218623(man1a2) 111235719 111237597(man1a1)
SLAL: 111651769 111665792(man1a2) 111672654(man1a1)
HCQ: 109510052(man1a2) 109515390 109523542(man1a1)
BPEC: 110156066(man1a2) 110166909(man1a1) 110174250
MALB: 109954665 109968853(man1a1) 109969891(man1a2)
ELS: 105006133 105012565 105016440(man1a2) 105017948(man1a1)
SFM: 108919178 108926840(man1a2) 108928530(man1a1) 108938572
PKI: 111836163(man1a1) 111844543 111855689 111856873(man1a2)
LCM: 102354592(MAN1C1) 102364126(MAN1A1) 102365619 102366726(MAN1A2)
CMK: 103179580 103180170(man1a2) 103182593(man1a1)
BFO: 118422544
CIN: 100185200
SPU: 581367
APLC: 110988207
SKO: 100376162
DME: Dmel_CG31202(alpha-Man-Ic) Dmel_CG42275(alpha-Man-Ia)
DSI: Dsimw501_GD17544(Dsim_GD17544) Dsimw501_GD24646(Dsim_GD24646)
DAN: 6505288
DAZ: 108620320
DNV: 115563934
DHE: 111593076
DVI: 6632126
LCQ: 111684411
AAG: 5578387
AALB: 109428360
AME: 100576383
BIM: 100745368
BTER: 100646409
CCAL: 108624370
OBB: 114877797
SOC: 105195935
MPHA: 105838260
AEC: 105150033
ACEP: 105620656
VEM: 105558151
HST: 105182725
DQU: 106748027
CFO: 105254509
LHU: 105669398
PGC: 109860716
OBO: 105286029
PCF: 106793692
NVI: 100119928
CSOL: 105366138
MDL: 103573956
TCA: 658773
DPA: 109534603
NVL: 108566967
PMAC: 106720767
PRAP: 110995197
HAW: 110383953
TNL: 113500731
API: 100163952
DNX: 107168850
AGS: 114132324
RMD: 113552584
BTAB: 109036238
CLEC: 106663826
ZNE: 110834785
PVM: 113800476
TUT: 107364560
DPTE: 113797529
CSCU: 111624473
PTEP: 107453286
CEL: CELE_C52E4.5(mans-2) CELE_D2030.1(mans-1) CELE_ZC410.3(mans-4)
BMY: Bm1_35990
TSP: Tsp_10255
PCAN: 112566007
CRG: 105330001
MYI: 110461635
OBI: 106870512
LAK: 106181834
SHX: MS3_08116
EGL: EGR_04601
NVE: 5510448
EPA: 110242380
ADF: 107355569
PDAM: 113678818
SPIS: 111334378
DGT: 114535692
HMG: 100211957
ATH: AT1G51590(MNS1) AT3G21160(MNS2)
CIT: 102615170
EGR: 104450686
LJA: Lj3g3v2055490.1(Lj3g3v2055490.1) Lj4g3v1983210.2(Lj4g3v1983210.2) Lj4g3v2139300.1(Lj4g3v2139300.1) Lj6g3v1318420.1(Lj6g3v1318420.1)
CSV: 101215522
CMO: 103500624
SIND: 105157913
OEU: 111401579
BVG: 104908666
SOE: 110785566
NNU: 104611388
OSA: 4336917
DOSA: Os04t0606400-01(Os04g0606400)
OBR: 102715848
BDI: 100824763
ATS: 109774541(LOC109774541)
DCT: 110110129
PEQ: 110023373
AOF: 109841366
ATR: 18435677
APRO: F751_5574
SCE: YLR057W(MNL2)
ERC: Ecym_1503
KMX: KLMA_20306(MNL2)
NCS: NCAS_0J00430(NCAS0J00430)
NDI: NDAI_0C06450(NDAI0C06450)
TPF: TPHA_0D00420(TPHA0D00420)
TBL: TBLA_0D02500(TBLA0D02500)
TDL: TDEL_0C00640(TDEL0C00640)
KAF: KAFR_0D01000(KAFR0D01000)
SLB: AWJ20_1894(MNL2)
NCR: NCU01059(gh47-3) NCU02235(gh47-6) NCU03134(gh47-2) NCU05836(gh47-4) NCU07067(gh47-5) NCU09028(gh47-8)
NTE: NEUTE1DRAFT111609(NEUTE1DRAFT_111609) NEUTE1DRAFT131655(NEUTE1DRAFT_131655) NEUTE1DRAFT51051(NEUTE1DRAFT_51051) NEUTE1DRAFT58018(NEUTE1DRAFT_58018) NEUTE1DRAFT67654(NEUTE1DRAFT_67654) NEUTE1DRAFT68020(NEUTE1DRAFT_68020)
ANG: ANI_1_1702014(An01g12550) ANI_1_180054(An06g01510) ANI_1_2010184(An04g06990)
CNE: CNG03330
CNB: CNBG1430
TASA: A1Q1_07106
ABP: AGABI1DRAFT115219(AGABI1DRAFT_115219) AGABI1DRAFT86588(AGABI1DRAFT_86588)
ABV: AGABI2DRAFT194180(AGABI2DRAFT_194180)
MRT: MRET_4105
DFA: DFA_08771
SMIN: v1.2.038650.t1(symbB.v1.2.038650.t1)
FCY: FRACYDRAFT_168118(MNS1)
CAK: Caul_4035
CSE: Cseg_0791
SCT: SCAT_4875
SEN: SACE_6225
FLM: MY04_4850
 » show all
Reference
1  [PMID:500665]
  Authors
Tabas I, Kornfeld S.
  Title
Purification and characterization of a rat liver Golgi alpha-mannosidase capable of processing asparagine-linked oligosaccharides.
  Journal
J Biol Chem 254:11655-63 (1979)
Reference
2  [PMID:7061502]
  Authors
Tulsiani DR, Hubbard SC, Robbins PW, Touster O.
  Title
alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates.
  Journal
J Biol Chem 257:3660-8 (1982)
Reference
3  [PMID:7588811]
  Authors
Bieberich E, Bause E
  Title
Man9-mannosidase from human kidney is expressed in COS cells as a Golgi-resident  type II transmembrane N-glycoprotein.
  Journal
Eur J Biochem 233:644-9 (1995)
DOI:10.1111/j.1432-1033.1995.644_2.x
Reference
4  [PMID:9592125]
  Authors
Tremblay LO, Campbell Dyke N, Herscovics A
  Title
Molecular cloning, chromosomal mapping and tissue-specific expression of a novel  human alpha1,2-mannosidase gene involved in N-glycan maturation.
  Journal
Glycobiology 8:585-95 (1998)
DOI:10.1093/glycob/8.6.585
Reference
5  [PMID:9719679]
  Authors
Lal A, Pang P, Kalelkar S, Romero PA, Herscovics A, Moremen KW
  Title
Substrate specificities of recombinant murine Golgi alpha1, 2-mannosidases IA and IB and comparison with endoplasmic reticulum and Golgi processing alpha1,2-mannosidases.
  Journal
Glycobiology 8:981-95 (1998)
DOI:10.1093/glycob/8.10.981
Reference
6  [PMID:10915796]
  Authors
Tremblay LO, Herscovics A
  Title
Characterization of a cDNA encoding a novel human Golgi alpha 1, 2-mannosidase (IC) involved in N-glycan biosynthesis.
  Journal
J Biol Chem 275:31655-60 (2000)
DOI:10.1074/jbc.M004935200
Reference
7  [PMID:19181540]
  Authors
Prien JM, Ashline DJ, Lapadula AJ, Zhang H, Reinhold VN
  Title
The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
  Journal
J Am Soc Mass Spectrom 20:539-56 (2009)
DOI:10.1016/j.jasms.2008.11.012
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.113
IUBMB Enzyme Nomenclature: 3.2.1.113
ExPASy - ENZYME nomenclature database: 3.2.1.113
BRENDA, the Enzyme Database: 3.2.1.113
CAS: 9068-25-1
LinkDB

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