KEGG   ENZYME: 3.2.1.155
Entry
EC 3.2.1.155                Enzyme                                 

Name
xyloglucan-specific endo-processive beta-1,4-glucanase;
Cel74A;
[(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan exo-glucohydrolase (ambiguous);
xyloglucan-specific exo-beta-1,4-glucanase (ambiguous)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
[(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan endo-processive glucohydrolase
Reaction(IUBMB)
Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the newly-formed chain end after endo-initiation on a polymer molecule
Comment
The enzyme removes branched oligosaccharides, containing preferentially four glucoside residues in the main chain, from xyloglucan molecules in a processive manner after the initial endo-type attack on a polysaccharide [1-5]. Hydrolysis occurs at either the unsubstituted D-glucopyranose residue in the main backbone and/or the D-glucopyranose residue bearing a xylosyl group [1-5]. The enzyme does not display activity, or shows very low activity, towards other beta-D-glucans [1,2,4,5].
History
EC 3.2.1.155 created 2005, withdrawn at public-review stage, modified and reinstated 2006, modified 2020
Orthology
K18578  xyloglucan-specific exo-beta-1,4-glucanase
Genes
NCR: NCU05955
NTE: NEUTE1DRAFT88314(NEUTE1DRAFT_88314)
SMP: SMAC_06979
PAN: PODANSg3079
TTT: THITE_2110055
MTM: MYCTH_116384
CTHR: CTHT_0040730
MGR: MGG_11553
TMN: UCRPA7_4728
FGR: FGSG_11208
ANI: AN5061.2
ANG: ANI_1_236014(An01g01870)
ABP: AGABI1DRAFT51133(AGABI1DRAFT_51133)
ABV: AGABI2DRAFT214617(AGABI2DRAFT_214617)
CJA: CJA_2477(gly74A)
BCON: NL30_17635
STAX: MC45_12315
PPY: PPE_02493
PPM: PPSC2_13345(celZ)
PPO: PPM_2561(celZ)
PPOL: X809_29760
PPOY: RE92_23455
PRI: PRIO_2780(xeg74)
CLT: CM240_3328(xghA)
CTH: Cthe_1398
HSC: HVS_14015(xghA5)
CCE: Ccel_1207
RCH: RUM_03220
CSC: Csac_1085
IDO: I598_1172
CFI: Celf_1913
KFL: Kfla_1830
ACE: Acel_0618
ACTI: UA75_19405
ACAD: UA74_18915
ASE: ACPL_2582
ACTN: L083_5389
AFS: AFR_18920
ACTS: ACWT_2455
GLJ: GKIL_4000
FBA: FIC_01257
 » show all
Reference
1  [PMID:15541296]
  Authors
Grishutin SG, Gusakov AV, Markov AV, Ustinov BB, Semenova MV, Sinitsyn AP.
  Title
Specific xyloglucanases as a new class of polysaccharide-degrading enzymes.
  Journal
Biochim Biophys Acta 1674:268-81 (2004)
DOI:10.1016/j.bbagen.2004.07.001
  Sequence
Reference
2  [PMID:22941084]
  Authors
Ichinose H, Araki Y, Michikawa M, Harazono K, Yaoi K, Karita S, Kaneko S
  Title
Characterization of an endo-processive-type xyloglucanase having a beta-1,4-glucan-binding module and an endo-type xyloglucanase from Streptomyces avermitilis.
  Journal
Appl Environ Microbiol 78:7939-45 (2012)
DOI:10.1128/AEM.01762-12
Reference
3  [PMID:24657616]
  Authors
Matsuzawa T, Saito Y, Yaoi K
  Title
Key amino acid residues for the endo-processive activity of GH74 xyloglucanase.
  Journal
FEBS Lett 588:1731-8 (2014)
DOI:10.1016/j.febslet.2014.03.023
Reference
4  [PMID:30463871]
  Authors
Arnal G, Stogios PJ, Asohan J, Skarina T, Savchenko A, Brumer H
  Title
Structural enzymology reveals the molecular basis of substrate regiospecificity and processivity of an exemplar bacterial glycoside hydrolase family 74 endo-xyloglucanase.
  Journal
Biochem J 475:3963-3978 (2018)
DOI:10.1042/BCJ20180763
Reference
5  [PMID:31324716]
  Authors
Arnal G, Stogios PJ, Asohan J, Attia MA, Skarina T, Viborg AH, Henrissat B, Savchenko A, Brumer H
  Title
Substrate specificity, regiospecificity, and processivity in glycoside hydrolase  family 74.
  Journal
J Biol Chem 294:13233-13247 (2019)
DOI:10.1074/jbc.RA119.009861
Reference
6  [PMID:31911243]
  Authors
Gusakov AV
  Title
Additional sequence and structural characterization of an endo-processive GH74 xyloglucanase from Myceliophthora thermophila and the revision of the EC 3.2.1.155 entry.
  Journal
Biochim Biophys Acta Gen Subj 1864:129511 (2020)
DOI:10.1016/j.bbagen.2020.129511
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.155
IUBMB Enzyme Nomenclature: 3.2.1.155
ExPASy - ENZYME nomenclature database: 3.2.1.155
BRENDA, the Enzyme Database: 3.2.1.155
CAS: 1000598-79-7
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