KEGG   ENZYME: 3.7.1.24Help
Entry
EC 3.7.1.24                 Enzyme                                 

Name
2,4-diacetylphloroglucinol hydrolase;
PhlG
Class
Hydrolases;
Acting on carbon-carbon bonds;
In ketonic substances
BRITE hierarchy
Sysname
2,4-diacetylphloroglucinol acetylhydrolase
Reaction(IUBMB)
2,4-diacetylphloroglucinol + H2O = 2-acetylphloroglucinol + acetate [RN:R12392]
Reaction(KEGG)
Substrate
2,4-diacetylphloroglucinol [CPD:C21896];
H2O [CPD:C00001]
Product
2-acetylphloroglucinol [CPD:C21895];
acetate [CPD:C00033]
Comment
Requires Zn2+. Isolated from the bacteria Pseudomonas fluorescens, Pseudomonas sp. YGJ3 and Mycobacterium abscessus 103. It reduces the antibiotic activity of 2,4-diacetylphloroglucinol.
History
EC 3.7.1.24 created 2019
Orthology
K23519  2,4-diacetylphloroglucinol hydrolase
Genes
SMW: SMWW4_v1c24760
SERF: L085_16355
ASU: Asuc_2081
PFL: PFL_5952(phlG)
PPRC: PFLCHA0_c59060(phlG)
PPRO: PPC_5899(phlG)
PFE: PSF113_2464(phlG)
PBA: PSEBR_a2324
PSOS: POS17_5894(phlG)
THD: BHV28_04310(phlG)
MAB: MAB_4487c
STRE: GZL_08194
SMAL: SMALA_7692
 » show all
Taxonomy
Reference
1  [PMID:16391073]
  Authors
Bottiglieri M, Keel C
  Title
Characterization of PhlG, a hydrolase that specifically degrades the antifungal compound 2,4-diacetylphloroglucinol in the biocontrol agent Pseudomonas fluorescens CHA0.
  Journal
Appl Environ Microbiol 72:418-27 (2006)
DOI:10.1128/AEM.72.1.418-427.2006
Reference
2  [PMID:20018877]
  Authors
He YX, Huang L, Xue Y, Fei X, Teng YB, Rubin-Pitel SB, Zhao H, Zhou CZ
  Title
Crystal structure and computational analyses provide insights into the catalytic  mechanism of 2,4-diacetylphloroglucinol hydrolase PhlG from Pseudomonas fluorescens.
  Journal
J Biol Chem 285:4603-11 (2010)
DOI:10.1074/jbc.M109.044180
  Sequence
[pfl:PFL_5952]
Reference
3  [PMID:22790955]
  Authors
Saitou H, Watanabe M, Maruyama K
  Title
Molecular and catalytic properties of 2,4-diacetylphloroglucinol hydrolase (PhlG) from Pseudomonas sp. YGJ3.
  Journal
Biosci Biotechnol Biochem 76:1239-41 (2012)
DOI:10.1271/bbb.120054
  Sequence
Reference
4  [PMID:25664803]
  Authors
Zhang Z, Jiang YL, Wu Y, He YX
  Title
Crystallization and preliminary X-ray diffraction analysis of a putative carbon-carbon bond hydrolase from Mycobacterium abscessus 103.
  Journal
Acta Crystallogr F Struct Biol Commun 71:239-42 (2015)
DOI:10.1107/S2053230X15001612
  Sequence
[mab:MAB_4487c]
Other DBs
ExplorEnz - The Enzyme Database: 3.7.1.24
IUBMB Enzyme Nomenclature: 3.7.1.24
ExPASy - ENZYME nomenclature database: 3.7.1.24
BRENDA, the Enzyme Database: 3.7.1.24
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