Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy

3-(methylsulfanyl)prop-2-enoyl-CoA hydro-lyase (acetaldehyde-forming)

3-(methylsulfanyl)acryloyl-CoA + 2 H2O = acetaldehyde + methanethiol + CoA + CO2 (overall reaction) [RN:R10936];
(1a) 3-(methylsulfanyl)acryloyl-CoA + H2O = 3-hydroxy-3-(methylsulfanyl)propanoyl-CoA [RN:R10945];
(1b) 3-hydroxy-3-(methylsulfanyl)propanoyl-CoA = 3-oxopropanoyl-CoA + methanethiol [RN:R10946];
(1c) 3-oxopropanoyl-CoA + H2O = 3-oxopropanoate + CoA [RN:R10947];
(1d) 3-oxopropanoate = acetaldehyde + CO2 [RN:R06973]

R06973 R10936 R10945 R10946 R10947
Reaction

3-(methylsulfanyl)acryloyl-CoA [CPD:C20955];
H2O [CPD:C00001];
3-hydroxy-3-(methylsulfanyl)propanoyl-CoA [CPD:C20968];
3-oxopropanoyl-CoA [CPD:C05989];
3-oxopropanoate [CPD:C00222]

The enzyme is involved in the degradation of 3-(dimethylsulfonio)propanoate, an osmolyte produced by marine phytoplankton. Isolated from the bacterium Ruegeria pomeroyi.

EC 4.2.1.155 created 2015

1  [PMID:23704947]

Tan D, Crabb WM, Whitman WB, Tong L

Crystal structure of DmdD, a crotonase superfamily enzyme that catalyzes the hydration and hydrolysis of methylthioacryloyl-CoA.

PLoS One 8:e63870 (2013)
DOI:10.1371/journal.pone.0063870

[sil:SPO3805]