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Entry
EC 4.4.1.30                 Enzyme                                 

Name
phycobiliprotein beta-cysteine-155 phycobilin lyase;
cpcT (gene name);
cpeT1 (gene name);
cpcT1 (gene name)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
BRITE hierarchy
Sysname
[phycobiliprotein beta-subunit]-Cys155-phycocyanobilin:phycocyanobilin lyase
Reaction(IUBMB)
(1) [C-phycocyanin beta-subunit]-Cys155-phycocyanobilin = apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin;
(2) [phycoerythrocyanin beta-subunit]-Cys155-phycocyanobilin = apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin
Substrate
[C-phycocyanin beta-subunit]-Cys155-phycocyanobilin;
[phycoerythrocyanin beta-subunit]-Cys155-phycocyanobilin
Product
apo-[C-phycocyanin beta-subunit];
(2R,3E)-phycocyanobilin;
apo-[phycoerythrocyanin beta-subunit]
Comment
The enzyme, found in cyanobacteria and red algae, catalyses the attachment of the phycobilin chromophore phycocyanobilin to cysteine 155 of the beta subunits of the phycobiliproteins C-phycocyanin and phycoerythrocyanin. The numbering used here corresponds to the enzyme from Anabaena, and could vary slightly in other organisms.
History
EC 4.4.1.30 created 2015
Reference
1  [PMID:17895251]
  Authors
Zhao KH, Zhang J, Tu JM, Bohm S, Ploscher M, Eichacker L, Bubenzer C, Scheer H, Wang X, Zhou M
  Title
Lyase activities of CpcS- and CpcT-like proteins from Nostoc PCC7120 and sequential reconstitution of binding sites of phycoerythrocyanin and phycocyanin  beta-subunits.
  Journal
J Biol Chem 282:34093-103 (2007)
DOI:10.1074/jbc.M703038200
  Sequence
[ana:all5339]
Reference
2  [PMID:24768724]
  Authors
Zhang R, Feng XT, Wu F, Ding Y, Zang XN, Zhang XC, Yuan DY, Zhao BR
  Title
Molecular cloning and expression analysis of a new bilin lyase: the cpcT gene encoding a bilin lyase responsible for attachment of phycocyanobilin to Cys-153 on the beta-subunit of phycocyanin in Arthrospira platensis FACHB314.
  Journal
Gene 544:191-7 (2014)
DOI:10.1016/j.gene.2014.04.050
Reference
3  [PMID:25074932]
  Authors
Zhou W, Ding WL, Zeng XL, Dong LL, Zhao B, Zhou M, Scheer H, Zhao KH, Yang X
  Title
Structure and mechanism of the phycobiliprotein lyase CpcT.
  Journal
J Biol Chem 289:26677-89 (2014)
DOI:10.1074/jbc.M114.586743
  Sequence
[ana:all5339]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.30
IUBMB Enzyme Nomenclature: 4.4.1.30
ExPASy - ENZYME nomenclature database: 4.4.1.30
BRENDA, the Enzyme Database: 4.4.1.30
LinkDB All DBs

DBGET integrated database retrieval system