Entry
Name
L-proline---[L-prolyl-carrier protein] ligase;
pltF (gene name);
bmp4 (gene name);
pigI (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
BRITE hierarchy
Sysname
L-proline:[L-prolyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-proline + holo-[L-prolyl-carrier protein] = AMP + diphosphate + L-prolyl-[L-prolyl-carrier protein] (overall reaction);
(1a) ATP + L-proline = diphosphate + (L-prolyl)adenylate [RN:
R12056 ];
(1b) (L-prolyl)adenylate + holo-[L-prolyl-carrier protein] = AMP + L-prolyl-[L-prolyl-carrier protein]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
L-proline [CPD:
C00148 ];
holo-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:
C20656 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
L-prolyl-[L-prolyl-carrier protein];
(L-prolyl)adenylate [CPD:
C20656 ]
Comment
The enzyme participates in the biosynthesis of several pyrrole-containing compounds, such as undecylprodigiosin, prodigiosin, pyoluteorin, and coumermycin A1. It catalyses the activation of L-proline to an adenylate form, followed by its transfer to the 4'-phosphopantheine moiety of an L-prolyl-carrier protein.
History
EC 6.2.1.53 created 2018
Pathway
ec01110 Biosynthesis of secondary metabolites
Orthology
K12719 L-proline---[L-prolyl-carrier protein] ligase
K21780 L-proline---[L-prolyl-carrier protein] ligase
Genes
SMW : SMWW4_v1c10960(pigI)
PPRC : PFLCHA0_c28500(tycC2)
TSV : DSM104635_00866(ppsD)
MSD : MYSTI_05336 MYSTI_06018
MFB : MFUL124B02_24105 MFUL124B02_29115
MLAN : JY572_36870 JY572_40520
PBRU : LZC95_20990 LZC95_48840
PRUE : LVJ94_20195 LVJ94_43385 LVJ94_49200
PALJ : LZC94_10615 LZC94_20060
SGM : GCM10017557_78140 GCM10017557_82750
SCYG : S1361_04950(tycC1) S1361_05215(lgrB1)
PSUU : Psuf_005010 Psuf_018720
» show all
Taxonomy
Reference
Authors
Thomas MG, Burkart MD, Walsh CT.
Title
Conversion of L-proline to pyrrolyl-2-carboxyl-S-PCP during undecylprodigiosin and pyoluteorin biosynthesis.
Journal
Reference
Authors
Harris AK, Williamson NR, Slater H, Cox A, Abbasi S, Foulds I, Simonsen HT, Leeper FJ, Salmond GP
Title
The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation.
Journal
Sequence
Reference
Authors
Williamson NR, Simonsen HT, Ahmed RA, Goldet G, Slater H, Woodley L, Leeper FJ, Salmond GP
Title
Biosynthesis of the red antibiotic, prodigiosin, in Serratia: identification of a novel 2-methyl-3-n-amyl-pyrrole (MAP) assembly pathway, definition of the terminal condensing enzyme, and implications for undecylprodigiosin biosynthesis in Streptomyces.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.53
ExPASy - ENZYME nomenclature database: 6.2.1.53
LinkDB
All DBs