Entry
Name
diphthine---ammonia ligase;
diphthamide synthase;
diphthamide synthetase;
DPH6 (gene name);
ATPBD4 (gene name);
diphthine:ammonia ligase (AMP-forming)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
BRITE hierarchy
Sysname
diphthine-[translation elongation factor 2]:ammonia ligase (AMP-forming)
Reaction(IUBMB)
ATP + diphthine-[translation elongation factor 2] + NH3 = AMP + diphosphate + diphthamide-[translation elongation factor 2] [RN:
R03613 ]
Reaction(KEGG)
Substrate
ATP [CPD:
C00002 ];
diphthine-[translation elongation factor 2] [CPD:
C01573 ];
NH3 [CPD:
C00014 ]
Product
AMP [CPD:
C00020 ];
diphosphate [CPD:
C00013 ];
diphthamide-[translation elongation factor 2]
Comment
This amidase catalyses the last step in the conversion of an L-histidine residue in the translation elongation factor EF2 to diphthamide. This factor is found in all archaea and eukaryota, but not in eubacteria, and is the target of bacterial toxins such as the diphtheria toxin and the Pseudomonas exotoxin A (see EC
2.4.2.36 , NAD+---diphthamide ADP-ribosyltransferase). The substrate of the enzyme, diphthine, is produced by EC
2.1.1.98 , diphthine synthase.
History
EC 6.3.1.14 created 1990 as EC 6.3.2.22, transferred 2010 to EC 6.3.1.14, modified 2013
Orthology
K06927 diphthine-ammonia ligase
Genes
OGA : 100958185(DPH6) 100958823
HHV : 120224227 120241885(DPH6)
HAI : 109386232(DPH6) 109386235
SHON : 118993625(DPH6) 118993721
EEE : 113572703 113591790(dph6)
MSAM : 119900178 119910319(dph6)
BPEC : 110161158(dph6) 110173883
ARUT : 117416705 117421836
DSI : Dsimw501_GD15968(Dsim_GD15968)
LCQ : 111678657 111689493 111689730
AALB : 109426358 115258087 115266292
CQU : CpipJ_CPIJ008398 CpipJ_CPIJ008400
HAME : 121859316 121866220
DPTE : 113788661 113794700
CSAT : 104701366 104744552 104764074
BNA : 106349636 106438981 106447631
DZI : 111277117 111296659 111296660
LJA : Lj1g3v3976130.1(Lj1g3v3976130.1) Lj1g3v3977140.1(Lj1g3v3977140.1)
AHF : 112696922 112696923 112754787 112757437
PALZ : 118036487 118050649
QSU : 112002671 112003069 112011407
SSPL : 121749653 121792384
PSOM : 113319064 113349896
DOSA : Os02t0810450-01(Os02g0810450)
TAES : 123128904 123139472 123146059
PVIR : 120645796 120657557
SMO : SELMODRAFT_130429 SELMODRAFT_134094
NDI : NDAI_0G02460(NDAI0G02460)
TPF : TPHA_0C00650(TPHA0C00650)
TBL : TBLA_0A04110(TBLA0A04110)
TDL : TDEL_0F04730(TDEL0F04730)
KAF : KAFR_0H02150(KAFR0H02150)
KNG : KNAG_0A02870(KNAG0A02870)
SPAA : SPAPADRAFT_60556 SPAPADRAFT_60558
CAL : CAALFM_C203830WA(CaO19.839)
NTE : NEUTE1DRAFT73167(NEUTE1DRAFT_73167)
PAN : PODANSg2029 PODANSg2030
VAL : VDBG_04681 VDBG_04682
BFU : BCIN_01g05150(Bcdph6)
ANG : ANI_1_1794184(An04g03970)
CGI : CGB_J0485W CGB_J0500W
ABP : AGABI1DRAFT38553(AGABI1DRAFT_38553)
ABV : AGABI2DRAFT67613(AGABI2DRAFT_67613)
SCM : SCHCO_02498721(SCHCODRAFT_02498721)
ACAN : ACA1_166560 ACA1_379050
PTM : GSPATT00008350001 GSPATT00013073001 GSPATT00015871001
SMIN : v1.2.008547.t1(symbB.v1.2.008547.t1)
SSIL : SOLI23_06825 SOLI23_07065
SOB : CSE16_17195 CSE16_17545
ACIT : HPK19_07920 HPK19_20370
DEV : DhcVS_1335(rdhH) DhcVS_95(rdhH)
DMG : GY50_0083 GY50_0092 GY50_0238 GY50_0243 GY50_1406
DMX : X792_00420 X792_00505 X792_00530 X792_07360 X792_07530
DMY : X793_01105 X793_01240
DMZ : X794_00985 X794_06575 X794_06790
DUC : UCH007_09910(rdhH) UCH007_13590(rdhH) UCH007_13800(rdhH)
DFO : Dform_01992 Dform_02039
DETH : HX448_03210 HX448_03360
METT : CIT01_00560 CIT01_00955
AFG : AFULGI_00008000 AFULGI_00019150
AST : Asulf_01658 Asulf_02236
PYC : TQ32_01380 TQ32_10715
TGY : X802_01995 X802_07610
THV : ADU37_CDS15740 ADU37_CDS19070
TCH : CHITON_0543 CHITON_1512
TCE : A3L02_03940 A3L02_05680
TPRF : A3L09_03250 A3L09_09485
TPAF : A3L08_00130 A3L08_05910
THY : A3L12_02075 A3L12_04905
PPAC : PAP_02520 PAP_03190
MBY : MSBRM_1435 MSBRM_2503
MBAR : MSBR2_0970 MSBR2_2481
MMAZ : MmTuc01_2410 MmTuc01_2873
MMJ : MSMAS_1914 MSMAS_2340
MMAC : MSMAC_1212 MSMAC_2105
MVC : MSVAZ_1776 MSVAZ_2553
MEK : MSKOL_1752 MSKOL_2561
NOU : Natoc_0679 Natoc_4175
NBG : DV706_01080 DV706_16190
PYW : PYWP30_00404 PYWP30_01304
ACIA : SE86_00240 SE86_02920
CLG : Calag_0969 Calag_1396
FFO : FFONT_0024 FFONT_0041 FFONT_1384
CCAI : NAS2_1008 NAS2_1478
PSYT : DSAG12_00331 DSAG12_03850
» show all
Taxonomy
Reference
1
Authors
Moehring, T.J. and Moehring, J.M.
Title
Mutant cultured cells used to study the synthesis of diphthamide.
Journal
UCLA Symp Mol Cell Biol New Ser 45:53-63 (1987)
Reference
Authors
Moehring JM, Moehring TJ.
Title
The post-translational trimethylation of diphthamide studied in vitro.
Journal
J Biol Chem 263:3840-4 (1988)
Reference
Authors
Su X, Lin Z, Chen W, Jiang H, Zhang S, Lin H
Title
Chemogenomic approach identified yeast YLR143W as diphthamide synthetase.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.14
ExPASy - ENZYME nomenclature database: 6.3.1.14
LinkDB
All DBs