Entry |
|
Name |
D-alanine---D-serine ligase;
VanC;
VanE;
VanG
|
Class |
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
|
Sysname |
D-alanine:D-serine ligase (ADP-forming)
|
Reaction(IUBMB) |
D-alanine + D-serine + ATP = D-alanyl-D-serine + ADP + phosphate [RN: R09595]
|
Reaction(KEGG) |
|
Substrate |
|
Product |
|
Comment |
The product of this enzyme, D-alanyl-D-serine, can be incorporated into the peptidoglycan pentapeptide instead of the usual D-alanyl-D-alanine dipeptide, which is formed by EC 6.3.2.4, D-alanine---D-alanine ligase. The resulting peptidoglycan does not bind the glycopeptide antibiotics vancomycin and teicoplanin, conferring resistance on the bacteria.
|
History |
EC 6.3.2.35 created 2010
|
Orthology |
K18856 | D-alanine---D-serine ligase |
|
Genes |
» show all
|
Reference |
|
Authors |
Dutka-Malen S, Molinas C, Arthur M, Courvalin P |
Title |
Sequence of the vanC gene of Enterococcus gallinarum BM4174 encoding a D-alanine:D-alanine ligase-related protein necessary for vancomycin resistance. |
Journal |
|
Sequence |
|
Reference |
|
Authors |
Park IS, Lin CH, Walsh CT |
Title |
Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase. |
Journal |
|
Reference |
|
Authors |
Fines M, Perichon B, Reynolds P, Sahm DF, Courvalin P |
Title |
VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405. |
Journal |
|
Reference |
|
Authors |
Depardieu F, Bonora MG, Reynolds PE, Courvalin P |
Title |
The vanG glycopeptide resistance operon from Enterococcus faecalis revisited. |
Journal |
|
Reference |
|
Authors |
Watanabe S, Kobayashi N, Quinones D, Hayakawa S, Nagashima S, Uehara N, Watanabe N |
Title |
Genetic diversity of the low-level vancomycin resistance gene vanC-2/vanC-3 and identification of a novel vanC subtype (vanC-4) in Enterococcus casseliflavus. |
Journal |
|
Other DBs |
ExplorEnz - The Enzyme Database: | 6.3.2.35 |
ExPASy - ENZYME nomenclature database: | 6.3.2.35 |
|
LinkDB |
|