KEGG   ENZYME: 1.1.1.365
Entry
EC 1.1.1.365                Enzyme                                 
Name
D-galacturonate reductase;
GalUR;
gar1 (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
L-galactonate:NADP+ oxidoreductase
Reaction(IUBMB)
L-galactonate + NADP+ = D-galacturonate + NADPH + H+ [RN:R07676]
Reaction(KEGG)
R07676
Substrate
L-galactonate [CPD:C15930];
NADP+ [CPD:C00006]
Product
D-galacturonate [CPD:C00333];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme from plants is involved in ascorbic acid (vitamin C) biosynthesis [1,2]. The enzyme from the fungus Trichoderma reesei (Hypocrea jecorina) is involved in a eukaryotic degradation pathway of D-galacturonate. It is also active with D-glucuronate and glyceraldehyde [3]. Neither enzyme shows any activity with NADH.
History
EC 1.1.1.365 created 2013
Pathway
ec00040  Pentose and glucuronate interconversions
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
Orthology
K19634  D-galacturonate reductase
K19642  D-galacturonate reductase
Genes
TCC18609968 18609969 18609970
GRA105794339
GHI107909951 107948305
GAB108488709
DZI111287841 111318131
EGR104432997
FVE101312822
RCN112179335 112180478 112197683
PPER18792656
PMUM103320996 103320997
PAVI110762797 110762798
PDUL117615044
MDM103403467 103403490 114820654
PXB103943126 103943129
CSV101206206 101220899 105434417
CMO103485931 103504508
BHJ120079163 120082065
MCHA111023026
CMAX111487459
CMOS111447573
CPEP111776980
HBR110634149
MESC110604780 110605509
JRE108996990
QSU112039299 112039897
QLO115979225 115982098
TWL120013951
VVI100233130 100254148
VRI117912187 117914996 117915195
SLY101256763
SPEN107016409
SOT102586306
SSTN125856242 125856604
NTA107770207 107822373
NSY104245176
NTO104110610
NAU109216145
INI109166850
ITR116003821
SIND105160165 105160170 105160182
OEU111399339
EGT105954242
SSPL121793704 121798253 121807646
HAN110870024 110872872 110872873 110872885 110872886 110894612 110894613 110894616 110894617
ECAD122578637 122581227 122586087 122608799 122610221
LSV111905193 111918309 111918314
CSIN114311083
NNU104588465
MING122078230
PSOM113293294 113360647
TRETRIREDRAFT_22004(gar1)
CHIGCH63R_10014
 » show all
Reference
1  [PMID:13363799]
  Authors
ISHERWOOD FA, MAPSON LW
  Title
Biological synthesis of ascorbic acid: the conversion of derivatives of D-galacturonic acid into L-ascorbic acid by plant extracts.
  Journal
Biochem J 64:13-22 (1956)
DOI:10.1042/bj0640013
Reference
2  [PMID:12524550]
  Authors
Agius F, Gonzalez-Lamothe R, Caballero JL, Munoz-Blanco J, Botella MA, Valpuesta V
  Title
Engineering increased vitamin C levels in plants by overexpression of a D-galacturonic acid reductase.
  Journal
Nat Biotechnol 21:177-81 (2003)
DOI:10.1038/nbt777
  Sequence
Reference
3  [PMID:16101307]
  Authors
Kuorelahti S, Kalkkinen N, Penttila M, Londesborough J, Richard P
  Title
Identification in the mold Hypocrea jecorina of the first fungal D-galacturonic acid reductase.
  Journal
Biochemistry 44:11234-40 (2005)
DOI:10.1021/bi050792f
  Sequence
Reference
4  [PMID:18768163]
  Authors
Martens-Uzunova ES, Schaap PJ
  Title
An evolutionary conserved d-galacturonic acid metabolic pathway operates across filamentous fungi capable of pectin degradation.
  Journal
Fungal Genet Biol 45:1449-57 (2008)
DOI:10.1016/j.fgb.2008.08.002
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.365
IUBMB Enzyme Nomenclature: 1.1.1.365
ExPASy - ENZYME nomenclature database: 1.1.1.365
BRENDA, the Enzyme Database: 1.1.1.365
LinkDB

DBGET integrated database retrieval system