KEGG ENZYME: 1.1.1.431

ENZYME: 1.1.1.431

Entry

EC 1.1.1.431                Enzyme

Name

D-xylose reductase (NADPH);
XYL1 (gene name, ambiguous);
xyl1 (gene name, ambiguous);
xyrA (gene name);
xyrB (gene name)

Class

Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor

Sysname

xylitol:NADP+ oxidoreductase

Reaction(IUBMB)

xylitol + NADP+ = D-xylose + NADPH + H+ [RN:R01431]

Reaction(KEGG)

R01431

Substrate

xylitol [CPD:C00379];
NADP+ [CPD:C00006]

Product

D-xylose [CPD:C00181];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

Xylose reductases catalyse the reduction of xylose to xylitol, the initial reaction in the fungal D-xylose degradation pathway. Most of the enzymes exhibit a strict requirement for NADPH (e.g. the enzymes from Saccharomyces cerevisiae, Aspergillus niger, Trichoderma reesei, Candida tropicalis, Saitozyma flava, and Candida intermedia). Some D-xylose reductases have dual cosubstrate specificity, though they still prefer NADPH to NADH (cf. EC 1.1.1.307, D-xylose reductase [NAD(P)H]). Very rarely the enzyme prefers NADH (cf. EC 1.1.1.430, D-xylose reductase (NADH)).

History

EC 1.1.1.431 created 2022

Orthology

K17743

D-xylose reductase

K26066

D-xylose/L-arabinose reductase (NADPH)

Genes

SASA:

100196319(xyl1)

SCE:	YHR104W(GRE3)

SEUB:

DI49_2509

SPAO:

SPAR_H01460

AGO:	AGOS_ACL107C

ERC:

Ecym_5508

KLA:	KLLA0_E21627g

KMX:	KLMA_10683(XYL1)

LTH:	KLTH0F16610g

ZRO:	ZYRO0A06336g

CGR:	2889331(GRE3)

NCS:	NCAS_0A06660(NCAS0A06660)

NDI:	NDAI_0D01600(NDAI0D01600)

TPF:	TPHA_0E01860(TPHA0E01860)

TBL:	TBLA_0A00630(TBLA0A00630) TBLA_0F01720(TBLA0F01720)

TGB:	HG536_0E00720

TDL:	TDEL_0E02150(TDEL0E02150)

KAF:	KAFR_0C05320(KAFR0C05320)

KNG:	KNAG_0B03780(KNAG0B03780)

ZMK:	HG535_0C03920

SLUD:

SCDLUD_000181

DHA:	DEHA2B00572g DEHA2E24244g DEHA2F13068g

PIC:	PICST_89614(XYL1)

PGU:	PGUG_00922 PGUG_02290 PGUG_05385

SPAA:

SPAPADRAFT_61339(XYL1.2) SPAPADRAFT_61341(XYL1.1)

LBG:	92210532(LODBEIA_P53360)

CAL:	CAALFM_C502930CA(GRE3)

CTP:	CTRG_05978 CTRG_05993

COT:	CORT_0F01440

CDU:	CD36_52690(XYRA)

CTEN:

18245566(PSN45_002368) 18250469(XYL1)

CLU:	CLUG_01135

CLUS:

A9F13_09g01958

CAUR:

CJI96_0001805 CJI96_0003286

ASAU:

88173380(PUMCH_002315)

PPA:	PAS_chr3_0744

PKZ:	C5L36_0B11990

BNN:	FOA43_003071(XYL1)

BBRX:

BRETT_005324(XYL1)

OPA:	HPODL_00132 HPODL_01370

YLI:	2910903(YALI2_D00962g)

SLB:	AWJ20_3140(GRE3)

NCR:	NCU08384(xr)

NTE:	NEUTE1DRAFT133088(NEUTE1DRAFT_133088)

PAN:	PODANSg09635

PBEL:

QC761_103840(xyl1)

PPSD:

QC762_103840(xyl1)

PPSP:

QC763_103840(xyl1)

PPSA:

QC764_103840(xyl1)

TTT:	THITE_2121064

MTM:	MYCTH_43671

CTHR:

CTHT_0056950

RTHE:

98124076(VTJ83DRAFT_309)

MGR:	MGG_01404 MGG_03648

PPEI:

PpBr36_00917 PpBr36_05674

PGRI:

PgNI_00350 PgNI_04112

TMN:	UCRPA7_3687 UCRPA7_6054 UCRPA7_6874

SSCK:

SPSK_06315 SPSK_10284

FGR:	FGSG_01523

FPU:	FPSE_05024

FPOA:

FPOAC1_001631(XYL1)

FVN:	FVRRES_01841

FVR:	FVEG_08194 FVEG_09602

FOX:	FOXG_01819 FOXG_11189

NHE:	NECHADRAFT_102983 NECHADRAFT_37143 NECHADRAFT_38020 NECHADRAFT_42050

FFC:	NCS54_00032000 NCS54_00545600

FKR:	NCS57_00030100 NCS57_00469500 NCS57_00583800

FMU:	J7337_000363(XYL1_1) J7337_002765(XYL1_2)

TRE:	TRIREDRAFT_107776(xyl1)

TRR:	M419DRAFT_94809

TATV:

25779059(TrAtP1_006040)

TASP:

36612655(XYL1) 36619134(TrAFT101_000120)

MAJ:

MAA_00542

MBRN:

26242741(xyl1)

PCHM:

VFPPC_01147 VFPPC_01327

CMT:	CCM_06357 CCM_08784

AMUS:

LMH87_002846 LMH87_007757

PLJ:	28888580(PLICBS_003754) 28891088(PLICBS_004598)

PTKZ:

JDV02_009626

VAL:	VDBG_00802 VDBG_03846 VDBG_06547

VDA:	VDAG_00411 VDAG_01073 VDAG_05087

CFJ:	CFIO01_01300 CFIO01_01602 CFIO01_09197 CFIO01_10020 CFIO01_11827

CLUP:

CLUP02_07368 CLUP02_13788 CLUP02_15584

CHIG:

CH63R_03776 CH63R_05876

CDET:

87940807(CDEST_04304) 87948860(CDEST_12360)

SAPO:

SAPIO_CDS7670 SAPIO_CDS9478

ELA:	UCREL1_14 UCREL1_6453 UCREL1_8867

PFY:	PFICI_01687 PFICI_04525 PFICI_05410 PFICI_06515 PFICI_07157 PFICI_10486

SSL:	SS1G_05911

BFU:	BCIN_05g01550

MBE:	MBM_04242 MBM_09727

PSCO:

LY89DRAFT_599386 LY89DRAFT_656415 LY89DRAFT_657917

GLZ:	GLAREA_03723

ANI:	ANIA_00423 ANIA_03312

AFM:	AFUA_1G04820 AFUA_6G13830

ACT:	ACLA_029850 ACLA_087030

NFI:	NFIA_019940 NFIA_059700

AOR:	AO090003000859 AO090103000359 AO090701000415

ANG:	An01g03740(xyrA) An08g08480 An08g09360

AFV:	AFLA_000817 AFLA_004930 AFLA_013173

ALUC:

AKAW2_20539A(XYL1) AKAW2_51678S

ACHE:

ACHE_10084A ACHE_50426S ACHE_50561S

APUU:

APUU_10973S APUU_11638A(XYL1) APUU_60796S APUU_60916A

PCS:	N7525_006115 N7525_010651 N7525_010789

PDP:	PDIP_20350

POU:	POX_e06956

TMF:	EYB26_006490 EYB26_008282

TRG:	TRUGW13939_00477 TRUGW13939_09110 TRUGW13939_09270

CIM:	CIMG_04915

CPW:	9696920(XYL1)

URE:	UREG_06636

ABE:

ARB_06141

TVE:

TRV_03808

PNO:	SNOG_12824

PTE:

PTT_08729

PTRR:

6347932(PtrM4_129140)

BZE:	COCCADRAFT_26499 COCCADRAFT_7101

BSC:	COCSADRAFT_41505 COCSADRAFT_90781

BOR:	COCMIDRAFT_103379 COCMIDRAFT_31692

AALT:

CC77DRAFT_925768

ADAC:

96085244(ACET3X_004922)

ARAB:

EKO05_0011455

ZTR:	MYCGRDRAFT_31902 MYCGRDRAFT_56833

PFJ:	MYCFIDRAFT_187110 MYCFIDRAFT_209507

FFU:	CLAFUR5_06085 CLAFUR5_06716

CBET:

CB0940_00153 CB0940_08334

BCOM:

BAUCODRAFT_374705

NPA:	UCRNP2_5371 UCRNP2_5917

TML:	GSTUM_00005097001

KMG:	30160407(I203_104460) 30162677(I203_101228)

CCAC:

CcaHIS019_0108390(xyl1)

TVS:	TRAVEDRAFT_161569 TRAVEDRAFT_68124

DSQ:	DICSQDRAFT_152560 DICSQDRAFT_95722

PCO:	PHACADRAFT_266555 PHACADRAFT_82194

SHS:	STEHIDRAFT_117277 STEHIDRAFT_124813

HIR:	HETIRDRAFT_59016

PSQ:	PUNSTDRAFT_95399

ADL:	AURDEDRAFT_181771

FME:	FOMMEDRAFT_145959

GTR:	GLOTRDRAFT_113068 GLOTRDRAFT_71965

RSX:	RhiXN_11579

CCI:	CC1G_12853

PCUB:

JR316_0009805

MPR:	MPER_01564

MRR:	Moror_16424

E1B28_001457

SCM:	SCHCO_02610865(SCHCODRAFT_02610865)

CPUT:

CONPUDRAFT_96186

SLA:	SERLADRAFT_455569

RIE:	OCT59_013315

» show all

Reference

1 [PMID:18553673]

Authors

Bolen PL, Detroy RW.

Title

Induction of NADPH-linked D-xylose reductase and NAD-linked xylitol dehydrogenase activities in Pachysolen tannophilus by D-xylose, L-arabinose, or D-galactose.

Journal

Biotechnol Bioeng 27:302-7 (1985)
DOI:10.1002/bit.260270314

Reference

2 [PMID:16232468]

Authors

Suzuki T, Yokoyama S, Kinoshita Y, Yamada H, Hatsu M, Takamizawa K, Kawai K.

Title

Expression of xyrA gene encoding for D-Xylose reductase of Candida tropicalis and production of xylitol in Escherichia coli.

Journal

J Biosci Bioeng 87:280-4 (1999)
DOI:10.1016/s1389-1723(99)80032-2

Sequence

[ag:BAA19476]

Reference

3 [PMID:10548539]

Authors

Nidetzky B, Mayr P, Hadwiger P, Stutz AE.

Title

Binding energy and specificity in the catalytic mechanism of yeast aldose reductases.

Journal

Biochem J 344 Pt 1:101-7 (1999)

Reference

4 [PMID:10681056]

Authors

Mayr P, Bruggler K, Kulbe KD, Nidetzky B.

Title

D-Xylose metabolism by Candida intermedia: isolation and characterisation of two forms of aldose reductase with different coenzyme specificities.

Journal

J Chromatogr B Biomed Sci Appl 737:195-202 (2000)
DOI:10.1016/s0378-4347(99)00380-1

Reference

5 [PMID:11963895]

Authors

Sene L, Felipe MG, Silva SS, Vitolo M.

Title

Preliminary kinetic characterization of xylose reductase and xylitol dehydrogenase extracted from Candida guilliermondii FTI 20037 cultivated in sugarcane bagasse hydrolysate for xylitol production.

Journal

Appl Biochem Biotechnol 91-93:671-80 (2001)
DOI:10.1385/abab:91-93:1-9:671

Reference

6 [PMID:11481678]

Authors

Jeong EY, Sopher C, Kim IS, Lee H.

Title

Mutational study of the role of tyrosine-49 in the Saccharomyces cerevisiae xylose reductase.

Journal

Yeast 18:1081-9 (2001)
DOI:10.1002/yea.758

Sequence

[sce:YHR104W]

Reference

7 [PMID:33666344]

Authors

Chroumpi T, Peng M, Aguilar-Pontes MV, Muller A, Wang M, Yan J, Lipzen A, Ng V, Grigoriev IV, Makela MR, de Vries RP.

Title

Revisiting a 'simple' fungal metabolic pathway reveals redundancy, complexity and diversity.

Journal

Microb Biotechnol 14:2525-2537 (2021)
DOI:10.1111/1751-7915.13790

Reference

8 [PMID:33842213]

Authors

Terebieniec A, Chroumpi T, Dilokpimol A, Aguilar-Pontes MV, Makela MR, de Vries RP.

Title

Characterization of d-xylose reductase, XyrB, from Aspergillus niger.

Journal

Biotechnol Rep (Amst) 30:e00610 (2021)
DOI:10.1016/j.btre.2021.e00610

Other DBs

ExplorEnz - The Enzyme Database:

1.1.1.431

IUBMB Enzyme Nomenclature:

1.1.1.431

ExPASy - ENZYME nomenclature database:

1.1.1.431

BRENDA, the Enzyme Database:

1.1.1.431

LinkDB

DBGET integrated database retrieval system