KEGG   ENZYME: 1.10.3.16
Entry
EC 1.10.3.16                Enzyme                                 
Name
dihydrophenazinedicarboxylate synthase;
phzG (gene name)
Class
Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor
Sysname
1,4,5a,6,9,10a-hexahydrophenazine-1,6-dicarboxylate:oxygen oxidoreductase
Reaction(IUBMB)
(1) (1R,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylate + O2 = (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate + H2O2 [RN:R11489];
(2) (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate + O2 = (5aS)-5,5a-dihydrophenazine-1,6-dicarboxylate + H2O2 [RN:R11490];
(3) (1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylate + O2 = (10aS)-10,10a-dihydrophenazine-1-carboxylate + H2O2 [RN:R11491];
(4) (1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate + O2 = (10aS)-5,10-dihydrophenazine-1-carboxylate + H2O2
Reaction(KEGG)
R11489 R11490 R11491;
(other) R11492
Substrate
(1R,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylate [CPD:C21408];
O2 [CPD:C00007];
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate [CPD:C21409];
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylate [CPD:C21411];
(1R)-1,4,5,10-tetrahydrophenazine-1-carboxylate [CPD:C21413]
Product
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylate [CPD:C21409];
H2O2 [CPD:C00027];
(5aS)-5,5a-dihydrophenazine-1,6-dicarboxylate [CPD:C21410];
(10aS)-10,10a-dihydrophenazine-1-carboxylate [CPD:C21412];
(10aS)-5,10-dihydrophenazine-1-carboxylate
Comment
Requires FMN. The enzyme, isolated from the bacteria Pseudomonas fluorescens 2-79 and Burkholderia lata 383, is involved in biosynthesis of the reduced forms of phenazine, phenazine-1-carboxylate, and phenazine-1,6-dicarboxylate, where it catalyses multiple reactions.
History
EC 1.10.3.16 created 2016
Pathway
ec00405  Phenazine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20262  dihydrophenazinedicarboxylate synthase
Genes
SFWWN53_03370
ECAECA2698(ehpE)
PATREV46_13180
PATOGZ59_18620(ehpE)
PPAVLOZ86_13685
LABLA76x_3331(phzG)
LAQGLA29479_2364(phzG)
PAEPA1905(phzG2) PA4216(phzG1)
PAEVN297_1965(pdxH) N297_4348(pdxH)
PAEIN296_1965(pdxH) N296_4348(pdxH)
PAUPA14_09410(phzG1) PA14_39880(phzG2)
PAPPSPA7_0883 PSPA7_3379
PAGPLES_07111(phzG1) PLES_34191(phzG2)
PAFPAM18_0721(phzG1) PAM18_3137(phzG2)
PNCNCGM2_2822(phzG1) NCGM2_5461(phzG1)
PAEBNCGM1900_0743(phzG1) NCGM1900_4584(phzG1)
PDKPADK2_03590
PSGG655_03625 G655_15590
PRPM062_09945 M062_22210
PAEPPA1S_03740 PA1S_16415
PAERPA1R_gp2107 PA1R_gp5534
PAEMU769_03770 U769_16130
PAELT223_03675 T223_17475
PAESSCV20265_0745 SCV20265_3490
PAEUBN889_04687(phzG2_1) BN889_06679(phzG2_2)
PAEGAI22_00170 AI22_17575
PAECM802_1963(pdxH) M802_4346(pdxH)
PAEOM801_1964(pdxH) M801_4214(pdxH)
PFBVO64_0340
POIBOP93_12290
PCHEY04_26300
PCPJM49_05445
PYMAK972_2559
PSEPC4K39_3095
BUUWS70_27195
BURBcep18194_B1572
BGLbglu_2g09280
BGUKS03_4989(phzG)
SECHB18_04225 B18_21415
CMVCMUST_14515
RERRER_pREL1-02980
SALSSLNWT_0234
STRCAA958_30785
STRDNI25_24415
SNWBBN63_05600
SQZFQU76_00270 FQU76_33470
SHAWCEB94_18890(pdxH)
SAQUEJC51_01035
SSEOD0Z67_01695(pdxH)
SCINCP977_15870(pdxH)
SHUNDWB77_04285(phzG)
SROIIAG44_40270(phzG)
SMAOCAG99_00505
LSEF1C12_15950(pdxH)
CUMNI26_14645
CUBBJK06_09775
CUGC1N91_14975(pdxH)
CQFGBG65_05105(pdxH)
CPHYB5808_16800
SALCC2138_10050(pdxH)
SALDFVA74_10700(pdxH)
HUMDVJ78_04080
PLAPEAO79_06655(pdxH)
CELHGXP71_13205(pdxH)
BRIFDF13_09775(pdxH)
THAONI17_009025(phzG)
NDANdas_5033
NOABKM31_19735
APRECNX65_16905(pdxH)
ACTAC1701_23220(pdxH)
ALOCRK58743
PRYPrubr_64710(phzG2)
NAVJQS30_14025(phzG)
 » show all
Reference
1  [PMID:23897464]
  Authors
Xu N, Ahuja EG, Janning P, Mavrodi DV, Thomashow LS, Blankenfeldt W
  Title
Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis.
  Journal
Acta Crystallogr D Biol Crystallogr 69:1403-13 (2013)
DOI:10.1107/S0907444913008354
Other DBs
ExplorEnz - The Enzyme Database: 1.10.3.16
IUBMB Enzyme Nomenclature: 1.10.3.16
ExPASy - ENZYME nomenclature database: 1.10.3.16
BRENDA, the Enzyme Database: 1.10.3.16
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