KEGG   ENZYME: 1.14.13.249
Entry
EC 1.14.13.249              Enzyme                                 
Name
3-amino-4-hydroxybenzoate 2-monooxygenase;
creL (gene name);
ptmB3 (gene name);
ptnB3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3-amino-4-hydroxybenzoate,NADPH:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
3-amino-4-hydroxybenzoate + NADPH + H+ + O2 = 3-amino-2,4-dihydroxybenzoate + NADP+ + H2O [RN:R12912]
Reaction(KEGG)
R12912
Substrate
3-amino-4-hydroxybenzoate [CPD:C12115];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
3-amino-2,4-dihydroxybenzoate [CPD:C22455];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Requires FAD. The CreL enzyme from the bacterium Streptomyces cremeus participates in the biosynthesis of cremeomycin. The PrmB3 and PtnB3 enzymes from Streptomyces platensis are involved in the biosynthesis of platensimycin and platencin, respectively.
History
EC 1.14.13.249 created 2021
Pathway
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K25563  3-amino-4-hydroxybenzoate 2-monooxygenase
Genes
SGRF: SGFS_074080
SDW: K7C20_14895
Reference
1  [PMID:21825154]
  Authors
Smanski MJ, Yu Z, Casper J, Lin S, Peterson RM, Chen Y, Wendt-Pienkowski E, Rajski SR, Shen B.
  Title
Dedicated ent-kaurene and ent-atiserene synthases for platensimycin and platencin biosynthesis.
  Journal
Proc Natl Acad Sci U S A 108:13498-503 (2011)
DOI:10.1073/pnas.1106919108
  Sequence
[ag:ACO31289]
Reference
2  [PMID:26278892]
  Authors
Waldman AJ, Pechersky Y, Wang P, Wang JX, Balskus EP.
  Title
The Cremeomycin Biosynthetic Gene Cluster Encodes a Pathway for Diazo Formation.
  Journal
Chembiochem 16:2172-5 (2015)
DOI:10.1002/cbic.201500407
  Sequence
[ag:ALA99209]
Reference
3  [PMID:26689788]
  Authors
Sugai Y, Katsuyama Y, Ohnishi Y.
  Title
A nitrous acid biosynthetic pathway for diazo group formation in bacteria.
  Journal
Nat Chem Biol 12:73-5 (2016)
DOI:10.1038/nchembio.1991
  Sequence
[ag:BAU09309]
Reference
4  [PMID:29915173]
  Authors
Dong LB, Rudolf JD, Kang D, Wang N, He CQ, Deng Y, Huang Y, Houk KN, Duan Y, Shen B.
  Title
Biosynthesis of thiocarboxylic acid-containing natural products.
  Journal
Nat Commun 9:2362 (2018)
DOI:10.1038/s41467-018-04747-y
Other DBs
ExplorEnz - The Enzyme Database: 1.14.13.249
IUBMB Enzyme Nomenclature: 1.14.13.249
ExPASy - ENZYME nomenclature database: 1.14.13.249
BRENDA, the Enzyme Database: 1.14.13.249
LinkDB

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