KEGG   ENZYME: 1.14.14.144
Entry
EC 1.14.14.144              Enzyme                                 
Name
abieta-7,13-diene hydroxylase;
abietadiene hydroxylase (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
abieta-7,13-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (18-hydroxylating)
Reaction(IUBMB)
abieta-7,13-diene + [reduced NADPH---hemoprotein reductase] + O2 = abieta-7,13-dien-18-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06351]
Reaction(KEGG)
R06351
Substrate
abieta-7,13-diene [CPD:C11878];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
abieta-7,13-dien-18-ol [CPD:C11882];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. This enzyme catalyses a step in the pathway of abietic acid biosynthesis. The activity has been demonstrated in cell-free stem extracts of Abies grandis (grand fir) and Pinus contorta (lodgepole pine). Activity is induced by wounding of the plant tissue [2].
History
EC 1.14.14.144 created 2009 as EC 1.14.13.108, modified 2012, transferred 2018 to EC 1.14.14.144
Pathway
ec00904  Diterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Reference
1  [PMID:8311462]
  Authors
Funk C, Croteau R
  Title
Diterpenoid resin acid biosynthesis in conifers: characterization of two cytochrome P450-dependent monooxygenases and an aldehyde dehydrogenase involved in abietic acid biosynthesis.
  Journal
Arch Biochem Biophys 308:258-66 (1994)
DOI:10.1006/abbi.1994.1036
Reference
2  [PMID:12232380]
  Authors
Funk C, Lewinsohn E, Vogel BS, Steele CL, Croteau R
  Title
Regulation of Oleoresinosis in Grand Fir (Abies grandis) (Coordinate Induction of Monoterpene and Diterpene Cyclases and Two Cytochrome P450-Dependent Diterpenoid Hydroxylases by Stem Wounding).
  Journal
Plant Physiol 106:999-1005 (1994)
DOI:10.1104/pp.106.3.999
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.144
IUBMB Enzyme Nomenclature: 1.14.14.144
ExPASy - ENZYME nomenclature database: 1.14.14.144
BRENDA, the Enzyme Database: 1.14.14.144
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