Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy

germacra-1(10),4,11(13)-trien-12-oate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (8alpha-hydroxylating)

germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH---hemoprotein reductase] + O2 = 8-epi-inunolide + [oxidized NADPH---hemoprotein reductase] + 2 H2O (overall reaction) [RN:R12275];
(1a) germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH---hemoprotein reductase] + O2 = 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R12273];
(1b) 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate = 8-epi-inunolide + H2O (spontaneous) [RN:R12274]

R12273 R12274 R12275
Reaction

germacra-1(10),4,11(13)-trien-12-oate [CPD:C19678];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate [CPD:C22051]

A cytochrome P-450 (heme-thiolate) protein from the plant Inula hupehensis. The enzyme also produces 8beta-hydroxygermacra-1(10),4,11(13)-triene-12-oate (EC 1.14.14.168, germacrene A acid 8beta-hydroxylase).

EC 1.14.14.170 created 2018

1  [PMID:29086444]