KEGG   ENZYME: 1.14.14.180
Entry
EC 1.14.14.180              Enzyme                                 
Name
brassinolide synthase;
CYP85A2 (gene name);
CYP85A3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
castasterone,NADPH---hemoprotein reductase:oxygen oxidoreductase (lactonizing, brassinolide-forming)
Reaction(IUBMB)
castasterone + O2 + [reduced NADPH---hemoprotein reductase] = brassinolide + 2 H2O + [oxidized NADPH---hemoprotein reductase] [RN:R07471]
Reaction(KEGG)
R07471;
(other) R08840 R08841
Substrate
castasterone [CPD:C15794];
O2 [CPD:C00007];
[reduced NADPH---hemoprotein reductase] [CPD:C03024]
Product
brassinolide [CPD:C08814];
H2O [CPD:C00001];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161]
Comment
This cytochrome P-450 (heme thiolate) plant enzyme catalyses the lactonization of several brassinosteroids, including castasterone, teasterone, and typhasterol. The CYP85A2 enzyme of Arabidopsis thaliana also catalyses the activity of EC 1.14.14.179, brassinosteroid 6-oxygenase.
History
EC 1.14.14.180 created 2022
Pathway
ec00905  Brassinosteroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12640  brassinosteroid 6-oxygenase / brassinolide synthase
K25959  brassinosteroid 6-oxygenase / brassinolide synthase
Genes
ATHAT3G30180(BR6OX2)
ALY9311596
CRB17885676
CSAT104710811 104780197 104790595
EUSEUTSA_v10004156mg
BRP103837220 103854484 103875063
BNA106346024 106349108 106363995 106397123 106400040 106410432 106410433 106411244
BOE106301844 106316572
RSZ108807315 108825972 108849348
THJ104805522 104822777
SLY100136888(CYP85A3)
SPEN107010718
SOT102599687
SSTN125848344
CANN107861006
 » show all
Reference
1  [PMID:15710611]
  Authors
Nomura T, Kushiro T, Yokota T, Kamiya Y, Bishop GJ, Yamaguchi S
  Title
The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis.
  Journal
J Biol Chem 280:17873-9 (2005)
DOI:10.1074/jbc.M414592200
  Sequence
[sly:100136888] [ath:AT3G30180]
Reference
2  [PMID:16024588]
  Authors
Kim TW, Hwang JY, Kim YS, Joo SH, Chang SC, Lee JS, Takatsuto S, Kim SK.
  Title
Arabidopsis CYP85A2, a cytochrome P450, mediates the Baeyer-Villiger oxidation of castasterone to brassinolide in brassinosteroid biosynthesis.
  Journal
Plant Cell 17:2397-412 (2005)
DOI:10.1105/tpc.105.033738
  Sequence
[ath:AT3G30180]
Reference
3  [PMID:18685225]
  Authors
Katsumata T, Hasegawa A, Fujiwara T, Komatsu T, Notomi M, Abe H, Natsume M, Kawaide H
  Title
Arabidopsis CYP85A2 catalyzes lactonization reactions in the biosynthesis of 2-deoxy-7-oxalactone brassinosteroids.
  Journal
Biosci Biotechnol Biochem 72:2110-7 (2008)
DOI:10.1271/bbb.80192
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.180
IUBMB Enzyme Nomenclature: 1.14.14.180
ExPASy - ENZYME nomenclature database: 1.14.14.180
BRENDA, the Enzyme Database: 1.14.14.180
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