KEGG   ENZYME: 1.14.14.190
Entry
EC 1.14.14.190              Enzyme                                 
Name
beta-colubrine 11-hydroxylase;
CYP71AH44
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
beta-colubrine,[NADPH---hemoprotein reductase]:oxygen oxidoreductase (11-hydroxylating)
Reaction(IUBMB)
beta-colubrine + [reduced NADPH---hemoprotein reductase] + O2 = 11-demethylbrucine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R13340]
Reaction(KEGG)
R13340
Substrate
beta-colubrine [CPD:C22930];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
11-demethylbrucine [CPD:C22931];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme, isolated from the tree Strychnos nux-vomica, participates in the biosynthesis of brucine.
History
EC 1.14.14.190 created 2024
Pathway
ec00901  Indole alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K27788  beta-colubrine 11-hydroxylase
Genes
ACAK: 137945373 137962174
Reference
1  [PMID:35794473]
  Authors
Hong B, Grzech D, Caputi L, Sonawane P, Lopez CER, Kamileen MO, Hernandez Lozada NJ, Grabe V, O'Connor SE
  Title
Biosynthesis of strychnine.
  Journal
Nature 607:617-622 (2022)
DOI:10.1038/s41586-022-04950-4
  Sequence
[ag:UQZ09629]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.190
IUBMB Enzyme Nomenclature: 1.14.14.190
ExPASy - ENZYME nomenclature database: 1.14.14.190
BRENDA, the Enzyme Database: 1.14.14.190
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