Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
taxa-4(20),11-dien-5alpha-yl acetate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (oxetane ring forming)
A cytochrome-P-450 (heme thiolate) enzyme. Demonstrated in the plant Taxus mairei (Maire's yew). It is involved in the biosynthesis of taxol (paclitaxel). The reaction involves epoxidation of the 4(20)-double bond followed by rearangement giving the oxetane ring. The reaction was demonstrated using taxa-4(20),11-diene-2alpha,5alpha,7beta,9alpha,10beta,13alpha-hexayl hexaacetate.
Jiang B, Gao L, Wang H, Sun Y, Zhang X, Ke H, Liu S, Ma P, Liao Q, Wang Y, Wang H, Liu Y, Du R, Rogge T, Li W, Shang Y, Houk KN, Xiong X, Xie D, Huang S, Lei X, Yan J.
Title
Characterization and heterologous reconstitution of Taxus biosynthetic enzymes leading to baccatin III.
