KEGG   ENZYME: 1.14.14.193
Entry
EC 1.14.14.193              Enzyme                                 
Name
taxol side chain-2'-hydroxylase;
CYP73A171;
T2'OH;
TB506
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
3'-N-debenzoyl-2'-deoxytaxol,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (2'-hydroxylating)
Reaction(IUBMB)
3'-N-debenzoyl-2'-deoxytaxol + [reduced NADPH-hemoprotein reductase] + O2 = 3'-N-debenzoyltaxol + [oxidized NADPH-hemoprotein reductase] + H2O [RN:R08173]
Reaction(KEGG)
R08173
Substrate
3'-N-debenzoyl-2'-deoxytaxol [CPD:C16523];
[reduced NADPH-hemoprotein reductase];
O2 [CPD:C00007]
Product
3'-N-debenzoyltaxol [CPD:C16524];
[oxidized NADPH-hemoprotein reductase];
H2O [CPD:C00001]
Comment
A cytochrome-P-450 (heme thiolate) enzyme isolated from the plant Taxus x media. The penultimate step in paclitaxel (taxol) biosynthesis.
History
EC 1.14.14.193 created 2025
Reference
1  [PMID:32528936]
  Authors
Sanchez-Munoz R, Perez-Mata E, Almagro L, Cusido RM, Bonfill M, Palazon J, Moyano E.
  Title
A Novel Hydroxylation Step in the Taxane Biosynthetic Pathway: A New Approach to Paclitaxel Production by Synthetic Biology.
  Journal
Front Bioeng Biotechnol 8:410 (2020)
DOI:10.3389/fbioe.2020.00410
Reference
2  [PMID:16137660]
  Authors
Long RM, Croteau R.
  Title
Preliminary assessment of the C13-side chain 2'-hydroxylase involved in taxol biosynthesis.
  Journal
Biochem Biophys Res Commun 338:410-7 (2005)
DOI:10.1016/j.bbrc.2005.08.119
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.193
IUBMB Enzyme Nomenclature: 1.14.14.193
ExPASy - ENZYME nomenclature database: 1.14.14.193
BRENDA, the Enzyme Database: 1.14.14.193
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