KEGG   ENZYME: 1.14.14.68
Entry
EC 1.14.14.68               Enzyme                                 
Name
syn-pimaradiene 3-monooxygenase;
CYP701A8
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
9beta-pimara7,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (9beta-pimara-7,15-diene-3beta-ol-forming)
Reaction(IUBMB)
9beta-pimara-7,15-diene + [reduced NADPH---hemoprotein reductase] + O2 = 9beta-pimara-7,15-diene-3beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11980]
Reaction(KEGG)
R11980
Substrate
9beta-pimara-7,15-diene [CPD:C18225];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
9beta-pimara-7,15-diene-3beta-ol [CPD:C21825];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from rice, Oryza sativa.
History
EC 1.14.14.68 created 2018
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K21719  ent-sandaracopimaradiene 3-hydroxylase / syn-pimaradiene 3-monooxygenase
Genes
OSA: 4341340(CYP701A9) 4341343(CYP701A8)
OBR: 102701085
OGL: 127776065 127776066
Reference
1  [PMID:25758958]
  Authors
Kitaoka N, Wu Y, Xu M, Peters RJ
  Title
Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis.
  Journal
Appl Microbiol Biotechnol 99:7549-58 (2015)
DOI:10.1007/s00253-015-6496-2
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.68
IUBMB Enzyme Nomenclature: 1.14.14.68
ExPASy - ENZYME nomenclature database: 1.14.14.68
BRENDA, the Enzyme Database: 1.14.14.68
LinkDB

DBGET integrated database retrieval system