Entry
Name
flavonoid 3'-monooxygenase;
CYP75B1 (gene name);
flavonoid 3'-hydroxylase;
flavonoid 3-hydroxylase (incorrect);
NADPH:flavonoid-3'-hydroxylase (incorrect);
flavonoid 3-monooxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
flavonoid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)
Reaction(IUBMB)
a flavonoid + [reduced NADPH---hemoprotein reductase] + O2 = a 3'-hydroxyflavonoid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
R03615 ]
Reaction(KEGG)
Substrate
flavonoid [CPD:
C01579 ];
[reduced NADPH---hemoprotein reductase] [CPD:
C03024 ];
O2 [CPD:
C00007 ]
Product
3'-hydroxyflavonoid [CPD:
C02790 ];
[oxidized NADPH---hemoprotein reductase] [CPD:
C03161 ];
H2O [CPD:
C00001 ]
Comment
A cytochrome P-450 (heme-thiolate) protein found in plants. Acts on a number of flavonoids, including the flavanone naringenin and the flavone apigenin. Does not act on 4-coumarate or 4-coumaroyl-CoA.
History
EC 1.14.14.82 created 1983 as EC 1.14.13.21, transferred 2018 to EC 1.14.14.82
Pathway
ec00944 Flavone and flavonol biosynthesis
ec01110 Biosynthesis of secondary metabolites
Orthology
K05280 flavonoid 3'-monooxygenase
Genes
CSAT : 104708304 104734816 104769003
DZI : 111289514 111290609 111303535
EGR : 104420423 104420432 104420450 120290178
GMX : 100777999 100778531 100779588 100807293 547475(SF3'H1)
GSJ : 114392946 114411811 114411812 114411813 114416612
VUM : 124828505 124830858 124830859
CCAJ : 109787887 109787893 109797283 109807203 109808745
APRC : 113846100 113848078 113848080 113848081 113852790
MTR : 11425400 11443726 25488708 25493879
TPRA : 123884828 123917490 123917493
PSAT : 127073732 127085494
VVO : 131604603 131627758 131627768 131634888 131638826 131656961 131656964 131659463 131662631 131662633 131662634 131662638
LJA : 130712022 130714133 130715267
LANG : 109344553 109353774
PCIN : 129308142 129308176 129309718 129309724 129320287
PAVI : 110753332 110762285
MSYL : 126599791 126625207
CSAV : 115709313 115709845 115709933
CAVE : 132174935 132176604
VVI : 100232999(F3'H) 100263810
NTA : 107773493(NtF3'H) 107795677(F3'H1)
SMIL : 131008844 131020359
APAN : 127240258 127240266 127255223 127255224 127255225
HAN : 110873618 110908215 110908322
ECAD : 122579393 122580625 122606651
PSOM : 113275817 113314103 113323649
BDI : 100832738 100840140 100844617 104583406
ATS : 109737789 109767455 109773560
TDC : 119295285 119319554 119322175 119329613 119338089 119349858 119366542
TAES : 123042898 123070997 123101915 123133137 123148568 123162411 123167460 123183410
TUA : 125518733 125526476 125532439
LPER : 127307462 127312600
LRD : 124667643 124684077 124684146 124687121
SBI : 8056468 8070594 8070599 8072541
ZMA : 100273383 100279887 103653707
MFLO : 136462318 136463721 136477613 136477614 136480697 136512247 136516893 136517003 136517739 136525850
SITA : 101759053 101763802
PVIR : 120650957 120651359
PHAI : 112878303 112903334
ZOF : 121970404 121975478 122042133 122046667
DCT : 110095936 110096779 110096781 110113263 110113268 110115941
PEQ : 110019582 110022396 110031953 110031993 110032025 110038151
AOF : 109819797 109835873 109837072
NCOL : 116256966 116257503 116257504 116257698 116257842
» show all
Taxonomy
Reference
1
Authors
Forkmann G, Heller W, Grisebach H.
Title
Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
Journal
Z Naturforsch C: Biosci 35:691-695 (1980)
Reference
Authors
Brugliera F, Barri-Rewell G, Holton TA, Mason JG.
Title
Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.
Journal
Sequence
Reference
Authors
Schoenbohm C, Martens S, Eder C, Forkmann G, Weisshaar B
Title
Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme.
Journal
Sequence
Other DBs
LinkDB
All DBs