KEGG   ENZYME: 1.14.14.82
Entry
EC 1.14.14.82               Enzyme                                 
Name
flavonoid 3'-monooxygenase;
CYP75B1 (gene name);
flavonoid 3'-hydroxylase;
flavonoid 3-hydroxylase (incorrect);
NADPH:flavonoid-3'-hydroxylase (incorrect);
flavonoid 3-monooxygenase (incorrect)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
flavonoid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)
Reaction(IUBMB)
a flavonoid + [reduced NADPH---hemoprotein reductase] + O2 = a 3'-hydroxyflavonoid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03615]
Reaction(KEGG)
Substrate
flavonoid [CPD:C01579];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
3'-hydroxyflavonoid [CPD:C02790];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in plants. Acts on a number of flavonoids, including the flavanone naringenin and the flavone apigenin. Does not act on 4-coumarate or 4-coumaroyl-CoA.
History
EC 1.14.14.82 created 1983 as EC 1.14.13.21, transferred 2018 to EC 1.14.14.82
Pathway
ec00941  Flavonoid biosynthesis
ec00944  Flavone and flavonol biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K05280  flavonoid 3'-monooxygenase
Genes
ATHAT5G07990(TT7)
ALY9309404
CRB17881114
CSAT104708304 104734816 104769003
EUSEUTSA_v10013289mg
BRP103847118
BNA106419056 111197716
BOE106313667
RSZ108814778
THJ104801243
CPAP110811144
CIT102620909
CICCICLE_v10019637mg
PVY116116966 116134244
MINC123204634
TCC18604820
GRA105764705
GHI107946535 107951234
GAB108479810
DZI111289514 111290609 111303535
EGR104420423 104420432 104420450
GMX100777999 100778531 100779588 100807293 547475(SF3'H1)
GSJ114392946 114411811 114411812 114411813 114416612
PVUPHAVU_003G1855001 PHAVU_009G192400g
VRA106756729 106766418
VAR108329986 108336235
VUN114164840
CCAJ109787887 109787893 109797283 109807203 109808745
APRC113846100 113848078 113848080 113848081 113852790
MTRMTR_3g024520 MTR_3g025230 MTR_3g025260 MTR_4g109470
CAM101507543 101510693
LJALj1g3v2293820.1(Lj1g3v2293820.1) Lj4g3v1708650.1(Lj4g3v1708650.1) Lj4g3v2267360.1(Lj4g3v2267360.1)
ADU107471019
AIP107623580
AHF112716295 112783881
LANG109344553 109353774
RCN112178545
PPER18777306
PMUM103338591
PAVI110753332 110762285
PDUL117627102
MDM103437875 103455413
PXB103930751
ZJU107419096
MNT21388201 21388202
CSV101207160
CMO103494036
BHJ120070880
CMAX111499893
CMOS111440987
CPEP111792334
RCU8284440
JCU105640611
HBR110635515 110636257
MESC110617654
POP112325778 7482418
PEU105115867
PALZ118047241
JRE108982908
QSU112039462
QLO115964033
TWL119981407 119987383
VVI100232999(F3'H) 100263810(F3'H)
VRI117904722
SLY101266618
SPEN107015315
SOT102593656
CANN107862334
NTA107773493(NtF3'H) 107795677(F3'H1)
NSY104221951 104236297
NTO104093246
NAU109223789
INI109160581(Magenta)
ITR116028975 116028976
SIND105157984
OEU111401587
EGT105958663 105973421
SSPL121762367
HAN110873618 110908215 110908322
ECAD122579393 122580625 122606651
LSV111891078 111908262
CCAV112514430
DCR108216209
CSIN114310487
BVG104907876
SOE110789886
CQI110700687 110726355
NNU104597960 104598992
MING122075299
TSS122654067
PSOM113275817 113314103 113323649
NCOL116256966 116257503 116257504 116257698 116257842
OSA4348299 4348304
DOSAOs10t0317900-01(Os10g0317900) Os10t0320100-01(Os10g0320100)
OBR102704205 102716721
BDI100832738 100840140 100844617 104583406
ATS109737789 109767455 109773560
TDC119295285 119319554 119322175 119329613 119338089 119349858 119366542
TAES123042898 123070997 123101915 123133137 123148568 123162411 123167460 123183410
TUA125518733 125526476 125532439
SBI8056468 8070594 8070599 8072541
ZMA100273383 100279887 103653707
SITA101759053 101763802
SVS117835112 117836868
PVIR120650957 120651359
PHAI112878303 112903334
PDA103708250 103710250
EGU105055971 105058071
MUS103979831
DCT110095936 110096779 110096781 110113263 110113268 110115941
PEQ110019582 110022396 110031953 110031993 110032025 110038151
AOF109819797 109835873 109837072
ATR18428045
 » show all
Reference
1
  Authors
Forkmann, G., Heller, W. and Grisebach, H.
  Title
Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
  Journal
Z Naturforsch C: Biosci 35:691-695 (1980)
Reference
2  [PMID:10504566]
  Authors
Brugliera F, Barri-Rewell G, Holton TA, Mason JG.
  Title
Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida.
  Journal
Plant J 19:441-51 (1999)
DOI:10.1046/j.1365-313X.1999.00539.x
  Sequence
Reference
3  [PMID:11030432]
  Authors
Schoenbohm C, Martens S, Eder C, Forkmann G, Weisshaar B
  Title
Identification of the Arabidopsis thaliana flavonoid 3'-hydroxylase gene and functional expression of the encoded P450 enzyme.
  Journal
Biol Chem 381:749-53 (2000)
DOI:10.1515/BC.2000.095
  Sequence
[ath:AT5G07990]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.82
IUBMB Enzyme Nomenclature: 1.14.14.82
ExPASy - ENZYME nomenclature database: 1.14.14.82
BRENDA, the Enzyme Database: 1.14.14.82
CAS: 75991-44-5
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