KEGG   ENZYME: 1.14.14.86
Entry
EC 1.14.14.86               Enzyme                                 

Name
ent-kaurene monooxygenase;
ent-kaurene oxidase (misleading)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
ent-kaur-16-ene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
ent-kaur-16-ene + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R10066];
(1a) ent-kaur-16-ene + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06291];
(1b) ent-kaur-16-en-19-ol + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R06292];
(1c) ent-kaur-16-en-19-al + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06293]
Reaction(KEGG)
Substrate
ent-kaur-16-ene [CPD:C06090];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Product
ent-kaur-16-en-19-oate [CPD:C11874];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Comment
A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
History
EC 1.14.14.86 created 2002 as EC 1.14.13.78, transferred 2018 to EC 1.14.14.86
Pathway
ec00904  Diterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K04122  ent-kaurene oxidase
K21292  ent-kaurene oxidase
Genes
CRG: 105335893 117682816
ATH: AT5G25900(GA3)
ALY: 9308254
CRB: 17884655
CSAT: 104706907 104736615 104771068
EUS: EUTSA_v10004043mg
BRP: 103874435
BNA: 106348557 106348758
BOE: 106301555
RSZ: 108833173 108853944
PVY: 116137812
MINC: 123229342
TCC: 18591283
GRA: 105774074
DZI: 111312649
GMX: 100802970(CYP701A16) 100814223
VRA: 106762922
VAR: 108336168
VUN: 114194654
APRC: 113857239
LJA: Lj0g3v0151459.1(Lj0g3v0151459.1) Lj0g3v0218449.1(Lj0g3v0218449.1) Lj3g3v0002210.2(Lj3g3v0002210.2)
FVE: 101315406
RCN: 112172744
PPER: 18791732
PAVI: 110762553
PDUL: 117616384
ZJU: 107420337
MNT: 21397575
CSV: 101209283
CMO: 103493505
BHJ: 120086045
MCHA: 111010641
RCU: 8260887
JCU: 105633700(EKO)
MESC: 110614910
POP: 18096306
PEU: 105120662
PALZ: 118028112
JRE: 108992364
TWL: 120009866
VVI: 100255742
VRI: 117907815
SLY: 101267112
SPEN: 107016073
NSY: 104230322
NTO: 104089658
NAU: 109224931
INI: 109149104(EKO1)
ITR: 116023321
EGT: 105965462
BVG: 104883634
SOE: 110783160
NNU: 104595848
NCOL: 116255470
DOSA: Os06t0569900-01(Os06g0569900) Os06t0570100-01(Os06g0570100)
OBR: 102719778
SBI: 8065021
MUS: 103986614
AOF: 109822294
ANI: AN1598.2
ANG: ANI_1_1208164(An18g02700) ANI_1_2452094(An11g09260)
ABE: ARB_00548
TVE: TRV_00376
PTE: PTT_08290
ZTR: MYCGRDRAFT_34461(CYP-58)
 » show all
Reference
1  [PMID:2223832]
  Authors
Ashman PJ, Mackenzie A, Bramley PM.
  Title
Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi.
  Journal
Biochim Biophys Acta 1036:151-7 (1990)
DOI:10.1016/0304-4165(90)90027-T
Reference
2  [PMID:1397591]
  Authors
Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM.
  Title
Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima.
  Journal
Biochem Soc Trans 20:218S (1992)
DOI:10.1042/bst020218s
Reference
3  [PMID:9952446]
  Authors
Helliwell CA, Poole A, Peacock WJ, Dennis ES.
  Title
Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
  Journal
Plant Physiol 119:507-10 (1999)
DOI:10.1104/pp.119.2.507
  Sequence
[ath:AT5G25900]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.86
IUBMB Enzyme Nomenclature: 1.14.14.86
ExPASy - ENZYME nomenclature database: 1.14.14.86
BRENDA, the Enzyme Database: 1.14.14.86
CAS: 149565-67-3
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