KEGG   ENZYME: 1.14.14.94
Entry
EC 1.14.14.94               Enzyme                                 
Name
leukotriene-B4 20-monooxygenase;
leukotriene-B4 20-hydroxylase;
leucotriene-B4 omega-hydroxylase;
LTB4 20-hydroxylase;
LTB4 omega-hydroxylase;
CYP4F2 (gene name);
CYP4F3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (20-hydroxylating)
Reaction(IUBMB)
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH---hemoprotein reductase] + O2 = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03866]
Reaction(KEGG)
R03866
Substrate
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate [CPD:C02165];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate [CPD:C04853];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in mammals.
History
EC 1.14.14.94 created 1989 as EC 1.14.13.30, transferred 2018 to EC 1.14.14.94
Pathway
ec00590  Arachidonic acid metabolism
Orthology
K17726  phylloquinone omega-hydroxylase / docosahexaenoic acid omega-hydroxylase / leukotriene-B4 20-monooxygenase
Genes
HSA: 4051(CYP4F3) 8529(CYP4F2)
PTR: 100609185 748117(CYP4F3) 748531(CYP4F2)
PPS: 100967411(CYP4F2) 100969141(CYP4F3)
GGO: 101125692 101135418 101152881
PON: 100434096 100437847 100453229
PPYG: 129020906 129020916
NLE: 100579320 100588006 115836968
HMH: 116478624 116478627
SSYN: 129459452 129460821
MCC: 718349 719317
MCF: 102130440(CYP4F3) 102134301(CYP4F2)
MTHB: 126942252 126942253
MNI: 105494188 105494717
CSAB: 103234079 103234089
CATY: 105601614 105601627
PANU: 101005940 101010705
TGE: 112612404 112613097
MLEU: 105540101 105540126
RRO: 104661012 104661014
RBB: 108543262 108543288 108543294
TFN: 117076174
PTEH: 111554233 111554287
CANG: 105519773 105519778
CJC: 100411160 100412575
SBQ: 101050944 101051361
CIMI: 108298561(CYP4F2)
ANAN: 105729220 105729221
CSYR: 103250633
MMUR: 105867925 105867928 105867931 105867933
LCAT: 123629269 123634142 123636956 123636960 123636967 123639775 123639780
PCOQ: 105810118 105825072 105825741 105827104
OGA: 100942215 100943471 100952962
CPOC: 100733735
OCU: 100348038 100350957
TUP: 102483333
GVR: 103593427 103610570
VLG: 121495723
UAR: 123779961 123780007
LLV: 125083145
ZCA: 113917975
MLX: 118005050
CHX: 102183680
BTAX: 128050384
ODA: 120864165
MLF: 102425173 102436085
MDT: 132234883 132234884
PKL: 118706555
DRO: 112305042
PDIC: 114503445
RFQ: 117038128
PGIG: 120606485
PVP: 105311420
RAY: 107517263
TOD: 119240020
LAV: 100657832 100659260
DNM: 101441145
 » show all
Reference
1  [PMID:3027095]
  Authors
Romano MC, Eckardt RD, Bender PE, Leonard TB, Straub KM, Newton JF.
  Title
Biochemical characterization of hepatic microsomal leukotriene B4 hydroxylases.
  Journal
J Biol Chem 262:1590-5 (1987)
Reference
2  [PMID:4044832]
  Authors
Shak S, Goldstein IM.
  Title
Leukotriene B4 omega-hydroxylase in human polymorphonuclear leukocytes. Partial purification and identification as a cytochrome P-450.
  Journal
J Clin Invest 76:1218-28 (1985)
DOI:10.1172/JCI112077
Reference
3  [PMID:2986111]
  Authors
Soberman RJ, Harper TW, Murphy RC, Austen KF.
  Title
Identification and functional characterization of leukotriene B4 20-hydroxylase of human polymorphonuclear leukocytes.
  Journal
Proc Natl Acad Sci U S A 82:2292-5 (1985)
DOI:10.1073/pnas.82.8.2292
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.94
IUBMB Enzyme Nomenclature: 1.14.14.94
ExPASy - ENZYME nomenclature database: 1.14.14.94
BRENDA, the Enzyme Database: 1.14.14.94
CAS: 90119-11-2
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