KEGG   ENZYME: 1.14.15.32
Entry
EC 1.14.15.32               Enzyme                                 
Name
pentalenene oxygenase;
PtlI
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
pentalenene,reduced ferredoxin:oxygen 13-oxidoreductase
Reaction(IUBMB)
pentalenene + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H+ + 2 O2 = pentalen-13-al + 4 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O (overall reaction) [RN:R09814];
(1a) pentalenene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pentalen-13-ol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R09812];
(1b) pentalen-13-ol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = pentalen-13-al + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O [RN:R09813]
Reaction(KEGG)
Substrate
pentalenene [CPD:C01841];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007];
pentalen-13-ol [CPD:C19938]
Product
pentalen-13-al [CPD:C19939];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
pentalen-13-ol [CPD:C19938]
Comment
A cytochrome P-450 (heme-thiolate) protein found in the bacterium Streptomyces avermitilis. The enzyme is involved in the biosynthesis of pentalenolactone and related antibiotics.
History
EC 1.14.15.32 created 2011 as EC 1.14.13.133, transferred 2018 to EC 1.14.15.32
Pathway
ec00909  Sesquiterpenoid and triterpenoid biosynthesis
ec00998  Biosynthesis of various antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15907  pentalenene oxygenase
Genes
SMASAVERM_2999(ptlI)
SCTSCAT_p0090(ptlI)
SCYSCATT_p16540
SBHSBI_09680(cyp48)
SDVBN159_1442(ptlI)
SCIB446_29700
SCWTU94_27410
SLEsle_13150(sle_13150)
SCLFBB341_00225
SSIAA7J05_09235
SALFSMD44_00690(ptlI)
SALJSMD11_0076(ptlI) SMD11_6153(ptlI)
SFEUIM697_14465
SGJIAG43_27160
SHKJ2N69_35800
KSKKSE_03600
 » show all
Reference
1  [PMID:17017767]
  Authors
Quaderer R, Omura S, Ikeda H, Cane DE
  Title
Pentalenolactone biosynthesis. Molecular cloning and assignment of biochemical function to PtlI, a cytochrome P450 of Streptomyces avermitilis.
  Journal
J Am Chem Soc 128:13036-7 (2006)
DOI:10.1021/ja0639214
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.32
IUBMB Enzyme Nomenclature: 1.14.15.32
ExPASy - ENZYME nomenclature database: 1.14.15.32
BRENDA, the Enzyme Database: 1.14.15.32
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