KEGG   ENZYME: 1.14.15.35
Entry
EC 1.14.15.35               Enzyme                                 

Name
6-deoxyerythronolide B hydroxylase;
DEB hydroxylase;
eryF (gene name);
P450(eryF);
CYP107A1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
6-deoxyerythronolide-B,reduced ferredoxin:oxygen oxidoreductase
Reaction(IUBMB)
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R05270]
Reaction(KEGG)
R05270
Substrate
6-deoxyerythronolide B [CPD:C03240];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
erythronolide B [CPD:C06635];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from the bacterium Saccharopolyspora erythraea. The enzyme is involved in the biosynthesis of the antibiotic erythromycin.
History
EC 1.14.15.35 created 2014 as EC 1.14.13.188, transferred 2018 to EC 1.14.15.35
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14366  6-deoxyerythronolide B hydroxylase
Genes
SNR: SNOUR_10185
AER: AERYTH_15880
SEN: SACE_0730(eryF)
AEY: CDG81_13145
Reference
1  [PMID:2011746]
  Authors
Weber JM, Leung JO, Swanson SJ, Idler KB, McAlpine JB
  Title
An erythromycin derivative produced by targeted gene disruption in Saccharopolyspora erythraea.
  Journal
Science 252:114-7 (1991)
DOI:10.1126/science.2011746
  Sequence
[sen:SACE_0730]
Reference
2  [PMID:2446657]
  Authors
Shafiee A, Hutchinson CR
  Title
Macrolide antibiotic biosynthesis: isolation and properties of two forms of 6-deoxyerythronolide B hydroxylase from Saccharopolyspora erythraea (Streptomyces erythreus).
  Journal
Biochemistry 26:6204-10 (1987)
DOI:10.1021/bi00393a037
Reference
3  [PMID:7846029]
  Authors
Cupp-Vickery JR, Li H, Poulos TL
  Title
Preliminary crystallographic analysis of an enzyme involved in erythromycin biosynthesis: cytochrome P450eryF.
  Journal
Proteins 20:197-201 (1994)
DOI:10.1002/prot.340200210
Reference
4  [PMID:15824115]
  Authors
Nagano S, Cupp-Vickery JR, Poulos TL
  Title
Crystal structures of the ferrous dioxygen complex of wild-type cytochrome P450eryF and its mutants, A245S and A245T: investigation of the proton transfer system in P450eryF.
  Journal
J Biol Chem 280:22102-7 (2005)
DOI:10.1074/jbc.M501732200
  Sequence
[sen:SACE_0730]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.35
IUBMB Enzyme Nomenclature: 1.14.15.35
ExPASy - ENZYME nomenclature database: 1.14.15.35
BRENDA, the Enzyme Database: 1.14.15.35
LinkDB

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