KEGG   ENZYME: 1.14.19.20
Entry
EC 1.14.19.20               Enzyme                                 

Name
Delta7-sterol 5(6)-desaturase;
lathosterol oxidase;
Delta7-sterol Delta5-dehydrogenase;
Delta7-sterol 5-desaturase;
Delta7-sterol-C5(6)-desaturase;
5-DES;
SC5DL (gene name);
ERG3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
Delta7-sterol,ferrocytochrome b5:oxygen oxidoreductase 5,6-dehydrogenating
Reaction(IUBMB)
a Delta7-sterol + 2 ferrocytochrome b5 + O2 + 2 H+ = a Delta5,7-sterol + 2 ferricytochrome b5 + 2 H2O
Reaction(KEGG)
(other) R07215 R07486 R07491 R07505
Substrate
Delta7-sterol;
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
Delta5,7-sterol;
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001]
Comment
This enzyme, found in eukaryotic organisms, catalyses the introduction of a double bond between the C5 and C6 carbons of the B ring of Delta7-sterols, to yield the corresponding Delta5,7-sterols. The enzymes from yeast, plants and vertebrates act on avenasterol, episterol, and lathosterol, respectively. The enzyme is located at the endoplasmic reticulum and is membrane bound.
History
EC 1.14.19.20 created 1972 as EC 1.3.3.2, transferred 2005 to EC 1.14.21.6, transferred 2015 to EC 1.14.19.20
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K00227  Delta7-sterol 5-desaturase
Genes
HSA: 6309(SC5D)
PTR: 451624(SC5D)
PPS: 100979606(SC5D)
GGO: 101151866(SC5D)
PON: 100459805(SC5D)
NLE: 100604476(SC5D)
MCC: 706827(SC5D)
MCF: 101867112(SC5D)
CSAB: 103248668(SC5D)
CATY: 105582529(SC5D)
PANU: 101015312(SC5D)
RRO: 104657234(SC5D)
RBB: 108523218(SC5D)
TFN: 117079880(SC5D)
CJC: 100387284(SC5D)
SBQ: 101038292(SC5D)
MMUR: 105885179(SC5D)
MMU: 235293(Sc5d)
MCAL: 110301807(Sc5d)
MPAH: 110327921(Sc5d) 110334695
RNO: 114100(Sc5d)
MCOC: 116072823(Sc5d)
MUN: 110540918(Sc5d)
CGE: 100752960(Sc5d)
PLEU: 114691145(Sc5d)
NGI: 103727913(Sc5d)
HGL: 101713479(Sc5d)
CCAN: 109698547(Sc5d)
OCU: 100358493(SC5D)
TUP: 102481940(SC5D)
CFA: 610411(SC5D)
VVP: 112920309(SC5D)
VLG: 121500224(SC5D)
AML: 100467645(SC5D)
UMR: 103662293(SC5D)
UAH: 113259940(SC5D)
ORO: 101367504(SC5D)
ELK: 111153897
MPUF: 101683752(SC5D)
EJU: 114226005(SC5D)
MLX: 118013329(SC5D)
FCA: 101099997(SC5D)
PTG: 102956406(SC5D)
PPAD: 109278494(SC5D)
AJU: 106976671(SC5D)
HHV: 120229652(SC5D)
BTA: 525154(SC5D)
BOM: 102283021(SC5D)
BIU: 109569702(SC5D)
BBUB: 102394495(SC5D)
CHX: 102180515(SC5D)
OAS: 101109313 101117697(SC5D)
ODA: 120876379(SC5D)
SSC: 100520925(SC5D)
CFR: 102520592(SC5D)
CDK: 105097554(SC5D)
BACU: 103004682(SC5D)
LVE: 103083016(SC5D)
OOR: 101282178(SC5D)
DLE: 111167467(SC5D)
PCAD: 102994174(SC5D)
ECB: 100063543(SC5D)
EPZ: 103543812(SC5D)
EAI: 106842373(SC5D)
MYB: 102246367(SC5D)
MYD: 102754805(SC5D)
MMYO: 118678221(SC5D)
MNA: 107534112(SC5D)
HAI: 109395291(SC5D)
DRO: 112301615 112301887(SC5D)
SHON: 118975222(SC5D)
AJM: 119039314(SC5D)
MMF: 118640120(SC5D)
PALE: 102891666(SC5D)
RAY: 107519049(SC5D)
MJV: 108389489 108409205(SC5D)
LAV: 100661853(SC5D)
TMU: 101351645
MDO: 100030841(SC5D)
SHR: 100932949(SC5D) 116419258
PCW: 110207244(SC5D)
OAA: 100073392(SC5D)
GGA: 100857756(SC5D)
PCOC: 116233108(SC5D)
MGP: 100538497(SC5D)
CJO: 107324219(SC5D)
NMEL: 110387590(SC5D)
APLA: 101803446(SC5D)
ACYG: 106039809(SC5D)
TGU: 100226384(SC5D)
LSR: 110477544(SC5D)
SCAN: 103823051(SC5D)
GFR: 102043549(SC5D)
FAB: 101811093(SC5D)
PHI: 102109366(SC5D)
PMAJ: 107214298(SC5D)
CCAE: 111939317(SC5D)
CCW: 104692196(SC5D)
ETL: 114066789(SC5D) 114072326
FPG: 101920444(SC5D)
FCH: 102057522(SC5D)
CLV: 102095349(SC5D)
EGZ: 104125957(SC5D)
NNI: 104016662(SC5D)
ACUN: 113488442(SC5D)
PADL: 103916253(SC5D)
AAM: 106490218(SC5D) 106491711
ASN: 102385911(SC5D)
AMJ: 102571716(SC5D)
CPOO: 109318043(SC5D)
GGN: 109292520(SC5D)
PSS: 102463426(SC5D)
CMY: 102931181(SC5D)
CPIC: 101952975(SC5D)
TST: 117868480(SC5D)
CABI: 116837729(SC5D)
ACS: 100566753(sc5d)
PVT: 110078414(SC5D)
PBI: 103051481(SC5D)
PMUR: 107285543(SC5D)
TSR: 106551450(SC5D)
PGUT: 117673231(SC5D)
PMUA: 114585161(SC5D)
ZVI: 118097123(SC5D)
GJA: 107125348(SC5D)
XLA: 108697607(sc5d.S) 447026(sc5d.L)
XTR: 100490845(sc5d)
NPR: 108797036(SC5D)
DRE: 447891(sc5d)
SANH: 107703513
IPU: 100528129(sc5d)
PHYP: 113541626(sc5d)
AMEX: 103032395(sc5d)
EEE: 113574240(sc5d)
TRU: 101067117(sc5d)
LCO: 104921101(sc5d) 104938430
ELY: 117261604 117271255(sc5d)
SLUC: 116052992 116056026(sc5d)
ONL: 100695064(sc5d) 100696185
OLA: 101173647(sc5d)
OML: 112141555(sc5d)
XMA: 102219081(sc5d)
XCO: 114153517(sc5d)
XHE: 116728775(sc5d)
PRET: 103476328(sc5d)
CVG: 107081853(sc5d)
NFU: 107387205(sc5d)
KMR: 108238026(sc5d)
CSEM: 103395378(sc5d)
POV: 109624686 109635651(sc5d)
LCF: 108891924(sc5d) 108901922
SDU: 111225164 111233090(sc5d)
SLAL: 111656091(sc5d) 111666108
XGL: 120803169(sc5d)
HCQ: 109515690(sc5d) 109526775
MALB: 109959324(sc5d)
SASA: 100195087(sc5d) 106576971
OTW: 112253002 112257983(sc5d)
OMY: 110521978 110537613(sc5d)
SALP: 111952019 111980773(sc5d)
ELS: 105023255 105024195(sc5d)
SFM: 108936096(sc5d)
PKI: 111844810(sc5d)
LOC: 102684690(sc5d)
PSPA: 121303985(sc5d)
LCM: 102365779(SC5D)
CMK: 103190397(sc5d)
RTP: 109938738(sc5d)
CIN: 100175543
SCLV: 120329567
SKO: 100375039
CSOL: 105363535
TCA: 103315179
DPA: 109535430
ATD: 109596276
APLN: 108734726
BMAN: 114240844
MSEX: 115450644
BANY: 112050995
PMAC: 106720255
PPOT: 106106039
PXU: 106121346
PRAP: 110998831
ZCE: 119838302
TNL: 113500445
API: 100163267
DNX: 107172322
NLU: 111047819
ZNE: 110831824
CSEC: 111865150
PVM: 113810916
HAME: 121876801
CSCU: 111625209
PCAN: 112567998
GAE: 121386508
PMAX: 117322145
PDAM: 113668803
SPIS: 111331638
DGT: 114523409
AQU: 100635449
ATH: AT3G02580(STE1) AT3G02590
CPAP: 110807494
CIT: 102630557
PVY: 116132598
TCC: 18602021
GRA: 105788590
GAB: 108489860
EGR: 104422113
VRA: 106765343
VAR: 108345518
VUN: 114181961
CCAJ: 109791074
APRC: 113867234
CAM: 101511716
LJA: Lj0g3v0103419.1(Lj0g3v0103419.1)
FVE: 101292472
RCN: 112174786
PPER: 18783760
PMUM: 103342673
PAVI: 110763174
PDUL: 117620560
MNT: 21409683
CSV: 101204738
CMO: 103487532
BHJ: 120082841
MCHA: 111016898
RCU: 8261209
JCU: 105638767
CANN: 107860069
SIND: 105177929
OSA: 4325687
DOSA: Os01t0134500-01(Os01g0134500)
OBR: 102707173
BDI: 100823877
ATS: 109738537
SBI: 8061354
SITA: 101780951
PHAI: 112893472
DCT: 110108874
ATR: 18432187
PPP: 112288804
SCE: YLR056W(ERG3)
ERC: Ecym_1501
KMX: KLMA_20308(ERG3)
NDI: NDAI_0C06430(NDAI0C06430)
TPF: TPHA_0D00440(TPHA0D00440) TPHA_0P00250(TPHA0P00250)
TBL: TBLA_0D02520(TBLA0D02520)
TDL: TDEL_0C00670(TDEL0C00670)
KAF: KAFR_0D01010(KAFR0D01010)
PIC: PICST_28450(ERG3)
SPAA: SPAPADRAFT_48108(ERG3)
CAL: CAALFM_C104770CA(ERG3)
CDU: CD36_04520(ERG3)
SLB: AWJ20_2868(ERG3)
NTE: NEUTE1DRAFT116882(NEUTE1DRAFT_116882) NEUTE1DRAFT78153(NEUTE1DRAFT_78153)
MGR: MGG_08832
SSCK: SPSK_02588
BFU: BCIN_16g04720(Bcerg3)
MBE: MBM_05730
ANG: ANI_1_28134(An15g00150) ANI_1_406144(An16g02930)
PTE: PTT_15444
ZTR: MYCGRDRAFT_74506(ERG3)
SPO: SPAC1687.16c(erg31) SPBC27B12.03c(erg32)
CNE: CNA05010
CNB: CNBA4830
TASA: A1Q1_03320
LBC: LACBIDRAFT_190564(ERG3)
ABP: AGABI1DRAFT59529(AGABI1DRAFT_59529)
ABV: AGABI2DRAFT218236(AGABI2DRAFT_218236)
MGL: MGL_0204
MRT: MRET_1111
DFA: DFA_02096
SMIN: v1.2.034753.t1(symbB.v1.2.034753.t1) v1.2.037901.t1(symbB.v1.2.037901.t1)
SDE: Sde_2984
HCH: HCH_01597
BPAR: BN117_0103
BPA: BPP0105
BPT: Bpet4857
AFQ: AFA_01575
ODI: ODI_R0206
DAC: Daci_1692
CFU: CFU_1702
RVA: Rvan_0861
JAN: Jann_3403
RDE: RD1_0610
PGD: Gal_00823
NAR: Saro_0236
SAL: Sala_3136
SPHP: LH20_01360
SPHU: SPPYR_3141
STAX: MC45_14775
SSAN: NX02_00960
SPKC: KC8_11295
SINB: SIDU_00390
SSY: SLG_31700
SPMI: K663_13655
SPHB: EP837_01002(erg3)
SPHR: BSY17_1944
SPHT: K426_17475
BLAS: BSY18_2315
SMIC: SmB9_24670
ALB: AEB_P0654
AAY: WYH_02232
BBAC: EP01_04755
MAR: MAE_29450
RUL: UC8_35600
FTJ: FTUN_4866
SACI: Sinac_3507
LBJ: LBJ_0847
LBL: LBL_2235
LST: LSS_15791
CPI: Cpin_1382
FLN: FLA_2486
MUC: MuYL_4794
SLI: Slin_3005
FAE: FAES_3530
PSEZ: HME7025_00151(erg3)
 » show all
Reference
1  [PMID:14189869]
  Authors
DEMPSEY ME, SEATON JD, SCHROEPFER GJ Jr, TROCKMAN RW.
  Title
THE INTERMEDIARY ROLE OF DELTA-5,7-CHOLESTADIEN-3-BETA-OL IN CHOLESTEROL BIOSYNTHESIS.
  Journal
J Biol Chem 239:1381-7 (1964)
Reference
2  [PMID:4019441]
  Authors
Honjo K, Ishibashi T, Imai Y
  Title
Partial purification and characterization of lathosterol 5-desaturase from rat liver microsomes.
  Journal
J Biochem 97:955-9 (1985)
DOI:10.1093/oxfordjournals.jbchem.a135137
Reference
3  [PMID:1864507]
  Authors
Arthington BA, Bennett LG, Skatrud PL, Guynn CJ, Barbuch RJ, Ulbright CE, Bard M
  Title
Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase.
  Journal
Gene 102:39-44 (1991)
DOI:10.1016/0378-1119(91)90535-J
  Sequence
[sce:YLR056W]
Reference
4  [PMID:8561508]
  Authors
Taton M, Rahier A.
  Title
Plant sterol biosynthesis: identification and characterization of higher plant delta 7-sterol C5(6)-desaturase.
  Journal
Arch Biochem Biophys 325:279-88 (1996)
DOI:10.1006/abbi.1996.0035
Reference
5  [PMID:9056262]
  Authors
Nishino H, Nakaya J, Nishi S, Kurosawa T, Ishibashi T.
  Title
Temperature-induced differential kinetic properties between an initial burst and the following steady state in membrane-bound enzymes: studies on lathosterol 5-desaturase.
  Journal
Arch Biochem Biophys 339:298-304 (1997)
DOI:10.1006/abbi.1996.9871
Reference
6  [PMID:10651635]
  Authors
Taton M, Husselstein T, Benveniste P, Rahier A.
  Title
Role of highly conserved residues in the reaction catalyzed by recombinant Delta7-sterol-C5(6)-desaturase studied by site-directed mutagenesis.
  Journal
Biochemistry 39:701-11 (2000)
DOI:10.1021/bi991467t
  Sequence
[ath:AT3G02580]
Reference
7  [PMID:22982564]
  Authors
Poklepovich TJ, Rinaldi MA, Tomazic ML, Favale NO, Turkewitz AP, Nudel CB, Nusblat AD
  Title
The cytochrome b5 dependent C-5(6) sterol desaturase DES5A from the endoplasmic reticulum of Tetrahymena thermophila complements ergosterol biosynthesis mutants  in Saccharomyces cerevisiae.
  Journal
Steroids 77:1313-20 (2012)
DOI:10.1016/j.steroids.2012.08.015
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.20
IUBMB Enzyme Nomenclature: 1.14.19.20
ExPASy - ENZYME nomenclature database: 1.14.19.20
BRENDA, the Enzyme Database: 1.14.19.20
CAS: 37255-37-1
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