KEGG   ENZYME: 1.14.19.43
Entry
EC 1.14.19.43               Enzyme                                 
Name
palmitoyl-[glycerolipid] 3-(E)-desaturase;
FAD4
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
1-acyl-2-palmitoyl-[glycerolipid],ferredoxin:oxygen oxidoreductase (3,4-trans -dehydrogenating)
Reaction(IUBMB)
a 1-acyl-2-palmitoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = a 1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
Substrate
1-acyl-2-palmitoyl-[glycerolipid];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
O2 [CPD:C00007];
H+ [CPD:C00080]
Product
1-acyl-2-[(3E)-hexadec-3-enoyl]-[glycerolipid];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
The enzyme introduces an unusual trans double bond at carbon 3 of a palmitoyl group attached to the sn-2 position of glycerolipids. The enzyme from the plant Arabidopsis thaliana is specific for palmitate in phosphatidylglycerol. The enzyme from tobacco can also accept oleate and alpha-linolenate if present at the sn-2 position of phosphatidylglycerol [1].
History
EC 1.14.19.43 created 2015
Orthology
K20417  palmitoyl-[glycerolipid] 3-(E)-desaturase
Genes
ATH: AT1G62190 AT2G22890 AT4G27030(FADA)
ALY: 9303600 9316535 9322552
CRB: 17880011 17889867 17894444
CSAT: 104702825 104713733 104714552 104717530 104722223 104730684 104751928 109125040
EUS: EUTSA_v10025879mg EUTSA_v10026787mg EUTSA_v10027264mg
BRP: 103834822 103835525 103861670 103866795
BNA: 106373945 106403010 106405551 106410515 106412662 106440906 106444859 106445558 111211564
BOE: 106303863 106308091 106310396 106310567 106321690
RSZ: 108821631 108822357 108826401 108852625 108854170 130508077
THJ: 104809192
CPAP: 110810962
CIT: 102608519
CIC: CICLE_v10009005mg
PVY: 116105927 116107733
MINC: 123201614
TCC: 18593565
GRA: 105780632
GHI: 107916746 107933433
GAB: 108480843
HSYR: 120159090 120160718
DZI: 111276443
EGR: 104448334
RARG: 115749908
GMX: 100793638 100795628
GSJ: 114394954 114424071
PVU: 137831173
VRA: 106775460
VAR: 108342269
VUN: 114187574
VUM: 124822779
CCAJ: 109805209
APRC: 113855030
MTR: 11423698
TPRA: 123917160
CAM: 101506527
PSAT: 127084935
VVO: 131633509 131635433
LJA: 130724042 130726147
ADU: 107479953
AIP: 107616614
AHF: 112720289 112789827
LANG: 109337761 109347611
PCIN: 129303737 129304063 129310784 129312191 129312192
QSA: O6P43_013737
FVE: 101296849
AANS: 126788795
RCN: 112165277
PPER: 18779734
PMUM: 103326516
PAVI: 110758161
PDUL: 117624771 117625430
MDM: 103434694 103448508
MSYL: 126588178 126616521
PXB: 103927610 103927630 103967035
PCOX: 137715084 137742698
ZJU: 107405836 107405839
MNT: 21400802
CSAV: 115694736 115698783 115724633
CSV: 101217074
CMO: 103501595
BHJ: 120091830
MCHA: 111019014
CMAX: 111476701
CMOS: 111447974
CPEP: 111779906
RCU: 8282921
MEAN: 126669293
JCU: 105632931
HBR: 110662372
MESC: 110601667
POP: 18095957 18103433
PEU: 105136537 105136540
PALZ: 118027850
PNZ: 133684498
JRE: 108983937
CILL: 122280451
CAVE: 132163744 132163745 132174553 132176451
AGLU: 133875780
QSU: 111996575 111996576 112012931
QLO: 115956364 115956367 115957374 115957450 115957451
TWL: 119990057
VVI: 100257229
VRI: 117909006
SLY: 101252202
SPEN: 107024217
SOT: 102586585
SSTN: 125876475
SDUL: 129892129
CANN: 107856304
LBB: 132616625 132617505 132617537 132617752 132618341
NTA: 107760919 107788973
NSY: 104243685
NTO: 104097800
NAU: 109228991
INI: 109190479
ITR: 116022715
SIND: 105166649 105175146 105176767 105176768
OEU: 111397714 111403959
EGT: 105948841 105961860 105961861
SSPL: 121755979 121786768
SMIL: 131011591 131011593 131024336 131024388
SHIS: 125192766 125209570
APAN: 127247385 127247386 127247833 127253218 127253373
HAN: 118492206 118492410
LSV: 111882811 111897379 111897388
CCAV: 112514210 112514211 112514212
DCR: 108211866
CSIN: 114297295
RVL: 131328745 131330474
AEW: 130773330 130789241
BVG: 104891650
SOE: 110776949 110782171
CQI: 110720983 110723537 110723571 110723814
ATRI: 130818310
MOF: 131163134
NNU: 104610901
MING: 122059443 122060224
TSS: 122641480
PSOM: 113287904 113304756
OSA: 107277927
OBR: 121055231
OGL: 127781185
BDI: 100826352
ATS: 109734181
TDC: 119315175 119324347
TAES: 123132194 123136224 123143728
TUA: 125513953 125513973
LPER: 127314668
LRD: 124654549 124675389
SBI: 8071119
ZMA: 103641109
MFLO: 136532549
SITA: 101754789 101755587
SVS: 117860181 117860572 117860774
PVIR: 120677787 120711630
PHAI: 112898030
PAUA: 133899617
PDA: 103720930
EGU: 105035002
MUS: 135625654
ZOF: 122001933 122003474
AOF: 109821617
MSIN: 131239880 131239892
ACAK: 137942567 137965403
NCOL: 116264693
ATR: 18448014
CJF: 131073028
SMO: SELMODRAFT_77032
PPP: 112290313
CRE: CHLRE_16g673001v5
MNG: MNEG_11576
CSL: COCSUDRAFT_11426
CVR: CHLNCDRAFT_137562
APRO: F751_3272
OTA: OT_ostta09g03220
BPG: Bathy10g01200
MIS: MICPUN_58724
MPP: MICPUCDRAFT_58905
GSL: Gasu_21010 Gasu_28340
FCY: FRACYDRAFT_177929
NGD: NGA_0194500
AAF: AURANDRAFT_67743 AURANDRAFT_67953
EHX: EMIHUDRAFT_453290
GTT: GUITHDRAFT_76752
GLO: Glov_2049
 » show all
Reference
1  [PMID:17158889]
  Authors
Fritz M, Lokstein H, Hackenberg D, Welti R, Roth M, Zahringer U, Fulda M, Hellmeyer W, Ott C, Wolter FP, Heinz E
  Title
Channeling of eukaryotic diacylglycerol into the biosynthesis of plastidial phosphatidylglycerol.
  Journal
J Biol Chem 282:4613-25 (2007)
DOI:10.1074/jbc.M606295200
Reference
2  [PMID:19682287]
  Authors
Gao J, Ajjawi I, Manoli A, Sawin A, Xu C, Froehlich JE, Last RL, Benning C
  Title
FATTY ACID DESATURASE4 of Arabidopsis encodes a protein distinct from characterized fatty acid desaturases.
  Journal
Plant J 60:832-9 (2009)
DOI:10.1111/j.1365-313X.2009.04001.x
  Sequence
[ath:AT4G27030]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.43
IUBMB Enzyme Nomenclature: 1.14.19.43
ExPASy - ENZYME nomenclature database: 1.14.19.43
BRENDA, the Enzyme Database: 1.14.19.43
LinkDB

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