KEGG ENZYME: 1.14.19.47

ENZYME: 1.14.19.47

Entry

EC 1.14.19.47               Enzyme

Name

acyl-lipid (9-3)-desaturase;
DES6 (gene name);
acyl-lipid 6-desaturase;
acyl-lipid Delta6-desaturase;
Delta6-desaturase (ambiguous)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water

Sysname

Delta9 acyl-[glycerolipid],ferrocytochrome b5:oxygen oxidoreductase (6,7-cis-dehydrogenating)

Reaction(IUBMB)

(1) an alpha-linolenoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a stearidonoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O [RN:R13058];
(2) a linoleoyl-[glycerolipid] + 2 ferrocytochrome b5 + O2 + 2 H+ = a gamma-linolenoyl-[glycerolipid] + 2 ferricytochrome b5 + 2 H2O [RN:R13059]

Reaction(KEGG)

R13058 R13059

Substrate

alpha-linolenoyl-[glycerolipid] [CPD:C21531];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
linoleoyl-[glycerolipid] [CPD:C21529]

Product

stearidonoyl-[glycerolipid] [CPD:C22564];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
gamma-linolenoyl-[glycerolipid] [CPD:C22563]

Comment

The enzyme, characterized from the moss Physcomitrella patens and the plant Borago officinalis (borage), introduces a cis double bond at carbon 6 of several acyl-lipids that contain an existing Delta9 cis double bond. The enzyme contains a cytochrome b5 domain that acts as the electron donor for the active site of the desaturase.

History

EC 1.14.19.47 created 2015

Orthology

K21737

acyl-lipid Delta6-acetylenase / acyl-lipid (9-3)-desaturase

Genes

BFO:	118409704 118409836

BBEL:

109462408 109469769

CIN:

100181774

SCLV:

120339347

NAI:	NECAME_05225

LOA:	LOAG_10800

LAK:	106165720 106175792 106175795

LLON:

135487842 135497573

NVE:

5520800

AMIL:

114962826

PDAM:

113673085

SPIS:

111333863

HSY:

130657637

RES:

135693404

TAD:	TRIADDRAFT_28957

THJ:

104803726

CPAP:

110822096

CIT:

102624404

CIC:	CICLE_v10010195mg

PVY:

116143292

MINC:

123195209 123201006

TCC:

18588068

GRA:	105769952 105792967

GHI:	107907009 107932872 107958876 121204795

GAB:	108459943 108467416

HSYR:

120132029 120133942 120156831 120163763

DZI:	111285207 111285209 111300415 111304116

EGR:

104436303

GMX:	100775880(SLD1.4) 100776476(SLD1.5)

GSJ:	114385103 114393364

PVU:	PHAVU_001G036200g

VRA:

106765081

VAR:

108336250

VUN:

114195260

VUM:

124842326

CCAJ:

109795310

APRC:

113846413

MTR:	112419175 11416346

TPRA:

123916771

CAM:

101502873

PSAT:

127097864

VVO:

131596398

LJA:

130717368

ADU:	107461234 107495639

AIP:	107605498 107606314

AHF:	112697662 112706370 112755914 112766680

LANG:

109328522 109343827

PCIN:

129299122 129303299 129317564

QSA:	O6P43_015900

FVE:

101310770

RCN:

112173700

PPER:

18789224

PMUM:

103320564

PAVI:

110764665

PDUL:

117616024

MDM:

103440767

MSYL:

126631793

PXB:

103952139

ZJU:

107420047

MNT:

21402436

CSAV:

115707010

RCU:

8259572

MEAN:

126669414

JCU:

105638142

HBR:

110633287

MESC:

110604075 110610280

POP:

7462256

PEU:

105122191

PALZ:

118030785

PNZ:

133689674

JRE:

109021249

CILL:

122297210

CAVE:

132184796

QSU:	136062896 136066015

QLO:

115970363

TWL:

120005399

VVI:

100259521

VRI:

117913126

SLY:

101257882

SPEN:

107028997

SOT:

102599871

SSTN:

125841119

SDUL:

129889236

CANN:

107864646 107867549

LBB:	132635909 132636622

NTA:	107768418 107798633(DXDES)

NSY:	104239037 104239380

NTO:

104121325

NAU:

109217138

INI:	109158372 109158482 109191355

ITR:	116005129 116005533 116006009

SIND:

105164669 105176919

OEU:	111373557 111375208

EGT:

105977192

SSPL:

121780413 121782620

SMIL:

131015384

SHIS:

125216312

APAN:

127265509

HAN:

110941481

ECAD:

122579280

LSV:

111906061

CCAV:

112504110

CSIN:

114300284 114319212

RVL:

131306707

AEW:	130756676 130766263

BVG:

104886626

MOF:

131150461

NNU:	104593449 104600267

MING:

122061951 122082529

TSS:	122648698 122664603

PSOM:

113299171 113322696 113340082 113343074 113358398

OSA:

4346776

OBR:

102714763

OGL:

127785481

BDI:

100826592

ATS:

109751130

TDC:	119301325 119308865

TAES:

123103524 123121294 542825

TUA:

125509111

LPER:

127300755

LRD:	124655067 124655095

SBI:	8054590 8075008

ZMA:	100191970 100279397 103651619

MFLO:

136541869

SITA:

101761789 101764528

SVS:	117845251 117855905

PVIR:

120660411 120676547

PHAI:

112882473

PDA:	103710903 103715999 103718541

EGU:	105040770 105041773 105048251

MUS:	103978403 103988108 103996075

ZOF:	121978954 121982615 121987241 122037872

DCT:	110096312 110112964 110112966 110116583

PEQ:	110020235 110035062 110037593

AOF:	109827972 109844045

MSIN:

131241322 131245040

NCOL:

116267278

ATR:

18436755

SMO:	SELMODRAFT_100447 SELMODRAFT_231254

PPP:	112277136 112282380 112284131

CSL:	COCSUDRAFT_47604

MIS:	MICPUN_109534

MPP:	MICPUCDRAFT_48880

CCP:	CHC_T00008879001

RIE:	OCT59_016741

TET:	TTHERM_00138530 TTHERM_00339850

PTM:	GSPATT00028900001

PTI:	PHATRDRAFT_29488(PTD6)

FCY:	FRACYDRAFT_228533(FAD2)

TPS:	THAPSDRAFT_41113(DES3)

AAF:	AURANDRAFT_28278

PIF:	PITG_05127

PSOJ:

PHYSODRAFT_356130

BLAC:

94348596(CCR75_004839)

SPAR:

SPRG_06919

LIF:	LINJ_36_7290

LDO:	LDBPK_367290

LMI:	LMXM_36_6950

LBZ:	LBRM_35_7330

LPAN:

LPMP_357160

» show all

Reference

1 [PMID:9108131]

Authors

Sayanova O, Smith MA, Lapinskas P, Stobart AK, Dobson G, Christie WW, Shewry PR, Napier JA

Title

Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6-desaturated fatty acids in transgenic tobacco.

Journal

Proc Natl Acad Sci U S A 94:4211-6 (1997)
DOI:10.1073/pnas.94.8.4211

Sequence

[ag:AAC49700]

Reference

2 [PMID:9744093]

Authors

Girke T, Schmidt H, Zahringer U, Reski R, Heinz E

Title

Identification of a novel delta 6-acyl-group desaturase by targeted gene disruption in Physcomitrella patens.

Journal

Plant J 15:39-48 (1998)
DOI:10.1046/j.1365-313X.1998.00178.x