KEGG   ENZYME: 1.14.19.6
Entry
EC 1.14.19.6                Enzyme                                 
Name
acyl-CoA (9+3)-desaturase;
oleoyl-CoA 12-desaturase;
Delta12 fatty acid desaturase;
Delta12(omega6)-desaturase;
oleoyl-CoA Delta12 desaturase;
Delta12 desaturase;
Delta12-desaturase;
Delta12-fatty-acid desaturase;
acyl-CoA,hydrogen donor:oxygen Delta12-oxidoreductase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
acyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (12,13 cis-dehydrogenating)
Reaction(IUBMB)
(1) oleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = linoleoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11043];
(2) palmitoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + 2 ferricytochrome b5 + 2 H2O [RN:R11044]
Reaction(KEGG)
R11043 R11044
Substrate
oleoyl-CoA [CPD:C00510];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080];
palmitoleoyl-CoA [CPD:C21072]
Product
linoleoyl-CoA [CPD:C02050];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001];
(9Z,12Z)-hexadeca-9,12-dienoyl-CoA [CPD:C21000]
Comment
This microsomal enzyme introduces a cis double bond at position 12 of fatty-acyl-CoAs that contain a cis double bond at position 9. When acting on 19:1Delta10 fatty acyl-CoA the enzyme from the pathogenic protozoan Trypanosoma brucei introduces the new double bond at position 13, indicating that the new double bond is introduced three carbons from the existing cis double bond, towards the methyl-end of the fatty acid. Requires cytochrome b5 as the electron donor [4].
History
EC 1.14.19.6 created 2008, modified 2015
Pathway
ec01040  Biosynthesis of unsaturated fatty acids
Orthology
K10256  omega-6 fatty acid desaturase / acyl-lipid omega-6 desaturase (Delta-12 desaturase)
K22993  bifunctional Delta-12/omega-3 fatty acid desaturase
Genes
AJM119047578
BTAB109031516 109034286 109037327 109038609 109038610 109038611 109043360 109043386 109043387 109043419 109043487 109043488 109043505
GAE121374992 121375004
HRF124116169
HRJ124268532 124268533
OSN115209645
NVE5512103
EPA110243700
ATEN116303763
ADF107344154
AMIL114948134 114973367
PDAM113667909 113682750
SPIS111329485 111342409
ATHAT3G12120(FAD2)
ALY9318834
CRB17890721
CSAT104745425 104764975 104776214
EUSEUTSA_v10020910mg
BRP103870276
BNA106417584 106418116 106452409 106452523 106453262 111197729 111197774
BOE106295108
RSZ108862913
THJ104799296 104826770
CPAP110811677
CIT102627239
CICCICLE_v10011949mg
PVY116125738 116136611
MINC123198158 123214921 123225237 123228010
TCC18589688 18599101
GRA105761671 105783314 105785452 105785453
GHI107901973 107921152 107921339 107924178 107934594 107941903 107945411 121212649
GAB108450424 108473778 108480547 108482302
DZI111295947 111304019 111311572
EGR104433804 104445836
GMX100780912(FAD2-2D) 100805040(FAD2-2B) 100805777(FAD2-1B) 100809290(FAD2-2A) 102662570 547814(FAD2-1A) 547815(FAD2-2) 732617(FAD2-3)
GSJ114367074 114370405 114400352 114401640 114406779
PVUPHAVU_001G142100g PHAVU_007G0234001 PHAVU_007G259500g
VRA106757695 106770720 106771256
VAR108323476 108325188 108325364
VUN114174864 114189943 114190784
CCAJ109794836 109795239 109796052 109796649
APRC113861993 113872359 113873750
MTRMTR_1g054035 MTR_1g111880 MTR_7g093200
CAM101489419 101507051 101513688
LJALj1g3v4317650.1(Lj1g3v4317650.1) Lj5g3v2237610.1(Lj5g3v2237610.1) Lj5g3v2237610.2(Lj5g3v2237610.2)
ADU107464693 107467337 107492893
AIP107616829 107619143 107645414
AHF112696114 112710390 112712140 112755017 112776164 112779709
LANG109328907 109331342 109345961 109346314 109347587
FVE101290788 101309231
RCN112194753 112201466
PPER18769718 18774949
PMUM103339420 103341921
PAVI110763023 110770740
PDUL117633799
MDM103427045 103443127 103454260
PXB103958977 103963937 103964864
ZJU107432351
MNT21386779 21391283 21401389
CSV101222623
CMO103486770
BHJ120088910 120090365
MCHA111024845
CMAX111470178 111481665 111484610 111490747
CMOS111441899 111448352 111448353 111459302
CPEP111777569 111777619 111786454 111786608 111789928 111795654 111800320
RCU8267086
JCU105646514
HBR110635031 110650724
MESC110621802 110622513
POP7479530
PEU105111221 105131774
PALZ118043755 118049781 118049805 118049806 118049817 118052395
JRE109001694 109011954 109021930
QSU111998386 112012330 112015494 112015748 112018769 112023137
QLO115952674 115977653
TWL119995107 119996264 119998754 120017269
VVI100252129
VRI117915771 117920052
SLY101250481 101259966 112941338
SPEN107003514 107015240
SOT102580407 102593159
CANN107843168 107849596 107849612 107851251 107851421 107851434 107851473 107851685 107851744 107873633
NTA107773163 107797104 107814090 107823061(FAD2) 107829678 107831844
NSY104226786 104230317 104246180
NTO104089719 104090741 104098987 104106672 104121261
NAU109215055 109215819 109216417 109216418 109218761 109235687
INI109150720 109152414 109182042
ITR116007185 116007582 116007943 116024476 116029392
SIND105159686 105163532(FAD2)
OEU111365935 111371390 111390764 111393798 111399693
EGT105951255 105955229
SSPL121747569 121751845 121794328 121798703
HAN110865031 110865032 110865239 110865277 110865281 110865284 110865285 110867681 110868684 110879372 110894444 110894736 110897314 110897316 110897318 110897319 110897568 110904312 110938218 110938220 110938221 110939886 110939887 110939961 110939971
ECAD122578445 122578764 122579373 122582919 122590523 122591112 122591269 122591356 122591604 122606452 122607353 122608062 122608674 122611246
LSV111879673 111882294 111884484 111885018 111889077 111889078 111889080 111889206 111890254 111896570 111896575 111896578 111896579 111896580 111896581 111896582 111897874 111899826 111900952 111900953 111904205 111908893 111909558 111915490 111918622
CCAV112505457 112505482 112505726 112506618 112506654 112506812 112506837 112506966 112506967 112506968 112512117 112513526 112521107 112521212 112521380 112521518 112521640 112521907 112521908 112521909 112521958 112525343 112526112 112527377 112527536 112527987 112528359 112528364
DCR108193493 108194523 108197230 108197256 108197419 108198376 108198633 108198634 108198635 108198637 108198638 108199375 108202677 108204656 108204690 108207522 108210933 108211018 108212237 108216232 108217089 108217440 108218481 108218482 108218742 108218743 108219326 108220169 108220600 108220695 108221065 108221821 108221989 108222165 108222186 108223766 108223875 108224006 108225347 108227781
CSIN114267865 114282301
BVG104889198 104889897
SOE110785100 110800912
CQI110689244 110695943 110734516 110736134
NNU104603541 104611778
MING122061917 122087967
TSS122642840 122642959
PSOM113311026 113322906
NCOL116257778
OSA4330523
DOSAOs02t0716500-01(Os02g0716500) Os07t0416700-00(Os07g0416700)
OBR102719739
ATS109756354 109768827 109774922 109774938 109781985
TDC119317653 119317654 119321176 119322537 119326524
TAES123128186 123130860 123130862 123134421 123134423 123138008 123145288 123145289 123145293 123145295
TUA125512392 125512434 125514359 125514360
SBI110435045 8066368
ZMA100282744 118475586
PVIR120650240 120657027 120677804 120701919
PDA103713931
EGU105050201
MUS103980416
DCT110101255 110108827
PEQ110023778 110033260
AOF109833613 109843629 109843630
ATR18439780
SMOSELMODRAFT_163663
PPP112278989 112291137
CRECHLRE_17g711150v5
VCNVOLCADRAFT_66373
MNGMNEG_7806
CVRCHLNCDRAFT_48812
CCPCHC_T00008296001
AGOAGOS_AFR589C
ERCEcym_2769
KLAKLLA0_F07095g
KMXKLMA_20392
LTHKLTH0D13640g
ZROZYRO0B07414g
TDLTDEL_0E01510(TDEL0E01510)
TGBHG536_0E01410
ZMKHG535_0F01710
PPAPAS_chr4_0052
DHADEHA2G05346g
PICPICST_72518(ODD1)
PGUPGUG_02928
SPAASPAPADRAFT_63149
LELLELG_04625
CALCAALFM_C601110WA(FAD2)
CTPCTRG_02975
COTCORT_0F04690
CDUCD36_61210
CTENCANTEDRAFT_114308
YLIYALI0B10153g
CLUCLUG_01582
CLUSA9F13_13g00209
CAURCJI97_004862
SLBAWJ20_1033(ODE1)
PKZC5L36_0C09550 C5L36_0C09560
BNNFOA43_000373
BBRXBRETT_002981
OPAHPODL_03473
NCRNCU02209(ods) NCU09497
NTENEUTE1DRAFT116751(NEUTE1DRAFT_116751) NEUTE1DRAFT86398(NEUTE1DRAFT_86398)
SMPSMAC_03650 SMAC_07882
PANPODANSg2430
TTTTHITE_76756
MTMMYCTH_50837 MYCTH_88639
CTHRCTHT_0000240 CTHT_0071750
MGRMGG_01985 MGG_08474
PPEIPpBr36_10589 PpBr36_10691
TMNUCRPA7_3220 UCRPA7_5047
SSCKSPSK_00128 SPSK_07620
FGRFGSG_05784 FGSG_07890
FPUFPSE_04515 FPSE_11043
FVRFVEG_05830 FVEG_12065
FOXFOXG_03793 FOXG_08835
NHENECHADRAFT_102202 NECHADRAFT_66003
TRETRIREDRAFT_45250 TRIREDRAFT_74228
TRRM419DRAFT_108308 M419DRAFT_26391
MAWMAC_06628
MAJMAA_07889
CMTCCM_06866 CCM_09560
PLJVFPFJ_10241 VFPFJ_10448
VALVDBG_10165
VDAVDAG_02971 VDAG_10469
CFJCFIO01_04749 CFIO01_09629
CHIGCH63R_11053 CH63R_12552
SAPOSAPIO_CDS0643 SAPIO_CDS7954
ELAUCREL1_10483 UCREL1_5610
PFYPFICI_02105 PFICI_05540 PFICI_11937
SSLSS1G_03438 SS1G_13683
BFUBCIN_01g05120 BCIN_01g10750 BCIN_02g06310 BCIN_13g00280
MBEMBM_04489 MBM_08304
PSCOLY89DRAFT_613354 LY89DRAFT_693845 LY89DRAFT_706947
GLZGLAREA_04493 GLAREA_09934
ANIAN1037.2 AN7204.2
AFMAFUA_1G12530 AFUA_6G03050
ACTACLA_022950 ACLA_097340
NFINFIA_013020 NFIA_049390
AORAO090001000224 AO090010000714
ANGANI_1_3412014(An01g14970) ANI_1_742074(An08g05160) ANI_1_962124(An14g06980)
AFVAFLA_008570 AFLA_014214
ALUCAKAW2_20019A AKAW2_30542S(FAH12) AKAW2_41307S
ACHEACHE_30343S(FAH12)
APUUAPUU_20896A APUU_50747S(FAH12)
PCSPc12g03490 Pc13g13170 Pc22g24730
PDPPDIP_27200 PDIP_61260
TMFPMAA_068450
TRGTRUGW13939_09539 TRUGW13939_10884
CIMCIMG_06871
CPWCPC735_032710
UREUREG_02885 UREG_03614
PBLPAAG_08655
PBNPADG_07259
ABEARB_01637
TVETRV_05152
AJEHCAG_01866
BGHBDBG_00166
PNOSNOG_01471
PTEPTT_13901
BZECOCCADRAFT_35692 COCCADRAFT_95357
BSCCOCSADRAFT_255949 COCSADRAFT_263376
BORCOCMIDRAFT_80232 COCMIDRAFT_99684
AALTCC77DRAFT_261211 CC77DRAFT_674296 CC77DRAFT_946470
ZTRMYCGRDRAFT_101437
PFJMYCFIDRAFT_72590 MYCFIDRAFT_86366
FFUCLAFUR5_03699 CLAFUR5_05071
BCOMBAUCODRAFT_24063
NPAUCRNP2_9247 UCRNP2_969
TMLGSTUM_00002898001
CNECND02750
CNBCNBD3600
CNGCNAG_01150
CGICGB_D5300C
TMSTREMEDRAFT_45585
TASAA1Q1_01239 A1Q1_06326
TVSTRAVEDRAFT_147014 TRAVEDRAFT_28463 TRAVEDRAFT_38834
DSQDICSQDRAFT_102286
PCOPHACADRAFT_265001
SHSSTEHIDRAFT_104093
HIRHETIRDRAFT_440056
PSQPUNSTDRAFT_141429
ADLAURDEDRAFT_115855
FMEFOMMEDRAFT_128530
GTRGLOTRDRAFT_61689
LBCLACBIDRAFT_292603(FAD12-5)
MRRMoror_13992
MOREE1B28_003350 E1B28_006255 E1B28_006258 E1B28_006259 E1B28_006748 E1B28_009359 E1B28_009360 E1B28_012456 E1B28_013509
CCICC1G_03449
SCMSCHCODRAFT_75336
ABPAGABI1DRAFT113988(AGABI1DRAFT_113988)
ABVAGABI2DRAFT193825(AGABI2DRAFT_193825)
CPUTCONPUDRAFT_56006
SLASERLADRAFT_469833
WSEWALSEDRAFT_64045
WICJ056_000579
UMAUMAG_11270
PFPPFL1_00921
MGLMGL_1345
MRTMRET_4015
MSYMMSY001_0308
PGRPGTG_00121
MLRMELLADRAFT_70376
SREPTSG_08157
NGDNGA_0081700(DES)
TBRTb927.2.3080
TBGTbgDal_II1450
TCR429257.20
LMALMJF_33_3270
LIFLINJ_33_3420
LDOLDBPK_333420
LMILMXM_32_3270
LBZLBRM_33_3550
LPANLPMP_101260 LPMP_333400
 » show all
Reference
1  [PMID:2248971]
  Authors
Borgeson CE, de Renobales M, Blomquist GJ.
  Title
Characterization of the delta 12 desaturase in the American cockroach, Periplaneta americana: the nature of the substrate.
  Journal
Biochim Biophys Acta 1047:135-40 (1990)
DOI:10.1016/0005-2760(90)90039-Z
Reference
2  [PMID:8900454]
  Authors
Lomascolo A, Dubreucq E, Galzy P.
  Title
Study of the delta 12-desaturase system of Lipomyces starkeyi.
  Journal
Lipids 31:253-9 (1996)
DOI:10.1007/BF02529871
Reference
3  [PMID:9829122]
  Authors
Tocher DR, Leaver MJ, Hodgson PA.
  Title
Recent advances in the biochemistry and molecular biology of fatty acyl desaturases.
  Journal
Prog Lipid Res 37:73-117 (1998)
DOI:10.1016/S0163-7827(98)00005-8
Reference
4  [PMID:15009186]
  Authors
Petrini GA, Altabe SG, Uttaro AD
  Title
Trypanosoma brucei oleate desaturase may use a cytochrome b5-like domain in another desaturase as an electron donor.
  Journal
Eur J Biochem 271:1079-86 (2004)
DOI:10.1111/j.1432-1033.2004.04005.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.6
IUBMB Enzyme Nomenclature: 1.14.19.6
ExPASy - ENZYME nomenclature database: 1.14.19.6
BRENDA, the Enzyme Database: 1.14.19.6
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