KEGG   ENZYME: 1.14.19.72
Entry
EC 1.14.19.72               Enzyme                                 

Name
(-)-pluviatolide synthase;
CYP719A23 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
(-)-matairesinol,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (methylenedioxy-bridge-forming)
Reaction(IUBMB)
(-)-matairesinol + [reduced NADPH---hemoprotein reductase] + O2 = (-)-pluviatolide + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11222]
Reaction(KEGG)
R11222
Substrate
(-)-matairesinol [CPD:C10682];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(-)-pluviatolide [CPD:C21191];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. The enzyme from the plants Sinopodophyllum hexandrum and Podophyllum peltatum catalyses the formation of a methylenedioxy-bridge. It is involved in the biosynthesis of podophyllotoxin, a non-alkaloid toxin lignan whose derivatives are important anticancer drugs.
History
EC 1.14.19.72 created 2016 as EC 1.14.21.11, transferred 2018 to EC 1.14.19.72
Pathway
ec00999  Biosynthesis of various plant secondary metabolites
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21207  (-)-pluviatolide synthase
Reference
1  [PMID:23161544]
  Authors
Marques JV, Kim KW, Lee C, Costa MA, May GD, Crow JA, Davin LB, Lewis NG
  Title
Next generation sequencing in predicting gene function in podophyllotoxin biosynthesis.
  Journal
J Biol Chem 288:466-79 (2013)
DOI:10.1074/jbc.M112.400689
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.72
IUBMB Enzyme Nomenclature: 1.14.19.72
ExPASy - ENZYME nomenclature database: 1.14.19.72
BRENDA, the Enzyme Database: 1.14.19.72
LinkDB

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