KEGG   ENZYME: 1.14.99.15
Entry
EC 1.14.99.15               Enzyme                                 
Name
4-methoxybenzoate monooxygenase (O-demethylating);
4-methoxybenzoate 4-monooxygenase (O-demethylating);
4-methoxybenzoate O-demethylase;
p-anisic O-demethylase;
piperonylate-4-O-demethylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
4-methoxybenzoate,hydrogen-donor:oxygen oxidoreductase (O-demethylating)
Reaction(IUBMB)
4-methoxybenzoate + reduced acceptor + O2 = 4-hydroxybenzoate + formaldehyde + acceptor + H2O [RN:R01306]
Reaction(KEGG)
R01306
Substrate
4-methoxybenzoate [CPD:C02519];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
4-hydroxybenzoate [CPD:C00156];
formaldehyde [CPD:C00067];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
The bacterial enzyme consists of a ferredoxin-type protein and an iron-sulfur flavoprotein (FMN). Also acts on 4-ethoxybenzoate, N-methyl-4-aminobenzoate and toluate. The fungal enzyme acts best on veratrate.
History
EC 1.14.99.15 created 1972
Pathway
ec00362  Benzoate degradation
ec01120  Microbial metabolism in diverse environments
Orthology
K22553  4-methoxybenzoate monooxygenase (O-demethylating)
Genes
PPUPP_1950
PPSHG5J76_09125
BHZACR54_04430
PUDG9Q38_04420 G9Q38_04600
PIGEGT29_07245
HYRBSY239_2681
HYLLPB072_21880
MAMOA6B35_30200
AVIAvi_7683
NGGRG540_PA03940
BJAblr1048(blr1048)
BJUBJ6T_10730
BJPRN69_05235
BRABRADO6810
BBTBBta_0729
BRSS23_67580
AOLS58_06520
BRCBCCGELA001_34415
BICLMTR13_36460
BROBRAD285_6957
BRKCWS35_02050
BOTCIT37_09060
BRQCIT40_30200
BGQX265_34795 X265_38440
BGZXH91_37640
BSYMCIT39_29310
BBETF8237_13145
BARHWN72_37625 WN72_46340
BVZBRAD3257_8678
BELBE61_89430 BE61_90500
BDGLPJ38_02285 LPJ38_14515
BSEPHAP48_0012045 HAP48_0019950
BQBJ4P68_0015900 J4P68_0023470
RPARPA1871
RPBRPB_3613
RPTRpal_2077
RPXRpdx1_3652
RBKE0H22_10435
VGOGJW-30_1_04086
TRBHB776_06670
MTUNMTUNDRAET4_0240.1(cyp199a)
PHRC6569_17635
PSTGE8M01_28530
PABYGa0080574_TMP462
SPSESULPSESMR1_04506
OCDFHY55_02300
HMLHmaOT1_13885
PPRUFDP22_01200
PPAFI8N54_16495
HDHG5B40_05810
NREBES08_14180
SWISwit_3269
SPHDHY78_09210
SARIH5J25_19695
SJPSJA_C2-04160
SCHSphch_3292
SSYSLG_20150
SPHBEP837_03852
SFLASPHFLASMR4Y_02994
SPHYCHN51_15135
AAYWYH_02150(bioI)
GTIFXF46_11520
ACEKFLP30_09705
SNEANBZ79_07865
MPAAMKK62_25030
MCROMI149_07690
MPHUMPHO_51270
CMDB841_09435
CCOECETAM_04400
CCYCSCMU_02260
RHARHA1_ro02948
ROPROP_26710 ROP_33150
ROAPd630_LPD07185
RAVAAT18_16735
RRZCS378_04375
RKOJWS14_13820 JWS14_15455
GPOGPOL_c45240
GOMD7316_02823
GAMGII34_05715
GOILK459_08650 LK459_20835 LK459_20945
SALSSLNWT_0027
SLEsle_61820(sle_61820)
SGEDWG14_00501
SGZC0216_11925
SRKFGW37_30985
SPHVF9278_42100
SBATG4Z16_06250
SXNIAG42_02270
SDECL3078_20680
AGYATC03_10810
SALDFVA74_07450
ARTArth_1727
ARYATC04_12915
ARZAUT26_19685
ACRYAC20117_03845 AC20117_16210
GLUF0M17_06450
GPRJQN66_15940
BLUTEW640_11445
PSIMKR76_01035
THAONI17_008420
ACTWF7P10_09220
BSDBLASA_2994
MMARMODMU_2722
AMQAMETH_2741(cypA)
AMYYYIM_24070(vdh1)
AORISD37_05135
AACDLWP59_14425
PDXPsed_3265
PSEAWY02_26855
PSEHXF36_15935
PSEQAD006_30685
PECQAD017_30555
PAUTPdca_32280
PBROHOP40_06445 HOP40_08630 HOP40_32540
SESPBN6_55130
AMSAMIS_8190
EKEEK0264_12805
 » show all
Reference
1  [PMID:188654]
  Authors
Bernhardt FH, Nastainczyk W, Seydewitz V.
  Title
Kinetic studies on a 4-methoxybenzoate O-demethylase from Pseudomonas putida.
  Journal
Eur J Biochem 72:107-15 (1977)
DOI:10.1111/j.1432-1033.1977.tb11230.x
Reference
2  [PMID:25369]
  Authors
Paszczynski A, Trojanowski J.
  Title
An affinity-column procedure for the purification of veratrate O-demethylase from fungi.
  Journal
Microbios 18:111-21 (1977)
Reference
3  [PMID:6273164]
  Authors
Twilfer H, Bernhardt FH, Gersonde K.
  Title
An electron-spin-resonance study on the redox-active centers of the 4-methoxybenzoate monooxygenase from Pseudomonas putida.
  Journal
Eur J Biochem 119:595-602 (1981)
DOI:10.1111/j.1432-1033.1981.tb05649.x
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.15
IUBMB Enzyme Nomenclature: 1.14.99.15
ExPASy - ENZYME nomenclature database: 1.14.99.15
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.99.15
BRENDA, the Enzyme Database: 1.14.99.15
CAS: 37256-78-3
LinkDB

DBGET integrated database retrieval system