KEGG   ENZYME: 1.14.99.38
Entry
EC 1.14.99.38               Enzyme                                 
Name
cholesterol 25-monooxygenase;
cholesterol 25-hydroxylase (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
cholesterol,hydrogen-donor:oxygen oxidoreductase (25-hydroxylating)
Reaction(IUBMB)
cholesterol + reduced acceptor + O2 = 25-hydroxycholesterol + acceptor + H2O [RN:R07218]
Reaction(KEGG)
R07218
Substrate
cholesterol [CPD:C00187];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
25-hydroxycholesterol [CPD:C15519];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P-450. Instead, it uses diiron cofactors to catalyse the hydroxylation of hydrophobic substrates [1]. The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues [4,5]. In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs). cf. EC 1.17.99.10, cholesterol C-25 hydroxylase.
History
EC 1.14.99.38 created 2005, modified 2020
Pathway
ec00120  Primary bile acid biosynthesis
Orthology
K10223  cholesterol 25-hydroxylase
Genes
HSA9023(CH25H)
PTR450584(CH25H)
PPS100974384(CH25H)
GGO101125247(CH25H)
PON100452872(CH25H)
NLE100583426(CH25H)
MCC694532(CH25H)
MCF102142173(CH25H)
CSAB103216226(CH25H)
CATY105587154(CH25H)
PANU101025055(CH25H)
TGE112631802(CH25H)
RRO104675146(CH25H)
RBB108526604(CH25H)
TFN117086228(CH25H)
PTEH111551335(CH25H)
CJC100405652(CH25H)
SBQ101034268(CH25H)
CSYR103273945(CH25H)
MMUR105871663(CH25H)
OGA100962631(CH25H)
MMU12642(Ch25h)
MCAL110286100(Ch25h)
MPAH110336789(Ch25h)
RNO309527(Ch25h)
MCOC116101152(Ch25h)
MUN110565101(Ch25h)
CGE100762824(Ch25h)
NGI103747462(Ch25h)
HGL101713638(Ch25h)
CPOC100726955(Ch25h)
CCAN109694732(Ch25h)
DORD105986298(Ch25h)
DSP122115977(Ch25h)
NCAR124985376
OCU100341039(CH25H)
OPI101528461(CH25H)
TUP102477002(CH25H)
CFA100856263(CH25H)
VVP112931975(CH25H)
VLG121475644(CH25H)
AML100470751(CH25H)
UMR103661935(CH25H)
UAH113258967(CH25H)
UAR123795495(CH25H)
ELK111145154
LLV125085217
MPUF101677681(CH25H)
ORO101385951(CH25H)
EJU114210228(CH25H)
ZCA113923929(CH25H)
MLX118012957(CH25H)
FCA101098893(CH25H)
PYU121016589(CH25H)
PBG122492669(CH25H)
PPAD109255418(CH25H)
AJU113596491(CH25H)
HHV120223792(CH25H)
BTA506132(CH25H)
BOM102271621(CH25H)
BIU109553211(CH25H)
BBUB102409279(CH25H)
CHX102191759(CH25H)
OAS101121645(CH25H)
ODA120873795(CH25H)
CCAD122446177(CH25H)
SSC100157868(CH25H)
CFR102516799(CH25H)
CBAI105081851(CH25H)
CDK105102833(CH25H)
VPC102529169(CH25H)
BACU103019491(CH25H)
LVE103080660(CH25H)
OOR101271130(CH25H)
DLE111172517(CH25H)
PCAD102984806(CH25H)
PSIU116741626(CH25H)
ECB100062138(CH25H)
EPZ103566587(CH25H)
EAI106831912(CH25H)
MYB102240160(CH25H)
MYD102760598(CH25H)
MMYO118669988(CH25H)
MLF102425166(CH25H)
MNA107543291(CH25H)
PKL118719016(CH25H)
HAI109377127(CH25H)
DRO112307321(CH25H)
SHON118987957(CH25H)
AJM119057716 119057746(CH25H)
PDIC114497800(CH25H)
PHAS123828950(CH25H)
MMF118632719(CH25H)
RFQ117036002(CH25H)
PALE102879471(CH25H)
PGIG120609344(CH25H)
PVP105293103(CH25H)
RAY107502210(CH25H)
MJV108392866(CH25H)
TOD119233913(CH25H)
SARA101550231(CH25H)
LAV100657141(CH25H)
TMU101357853
DNM101411736 101414027 101415306 101421920 101426258 101442861 101446245 101446828
GAS123255338(CH25H)
SHR100930151(CH25H)
PCW110198978(CH25H)
OAA100078582(CH25H)
GGA423788(CH25H)
PCOC116238153(CH25H)
MGP100550290(CH25H)
CJO107315869(CH25H)
NMEL110399914(CH25H)
APLA101794901(CH25H)
ACYG106044273(CH25H)
AFUL116491509(CH25H)
TGU100222744(CH25H)
LSR110475749(CH25H)
SCAN103813932(CH25H)
PMOA120502037(CH25H)
OTC121341848(CH25H)
PRUF121350187(CH25H)
GFR102033532(CH25H)
FAB101806492(CH25H)
PHI102102616(CH25H) 102109333
PMAJ107206999(CH25H)
CCAE111931167(CH25H)
CCW104696497(CH25H)
ETL114055897(CH25H)
ZAB102072815(CH25H)
FPG101915580(CH25H)
FCH102060118(CH25H)
CLV102084518(CH25H)
EGZ104129156(CH25H)
NNI104013120(CH25H)
ACUN113481803(CH25H)
TALA104363239(CH25H)
PADL103913652(CH25H)
ACHC115348512(CH25H)
AAM106487843(CH25H)
AROW112975233(CH25H)
NPD112957470(CH25H)
DNE112979724(CH25H)
ASN102371917(CH25H)
AMJ102561874(CH25H)
CPOO109324314(CH25H)
GGN109287470(CH25H)
PSS102456208(CH25H)
CMY102939174(CH25H)
CPIC101938379(CH25H)
TST117880266(CH25H)
CABI116818987(CH25H)
MRV120369311(CH25H)
ACS100554078 100568259(ch25h)
PVT110070876(CH25H)
SUND121926373(CH25H)
PBI103058588(CH25H)
PMUR107293838(CH25H)
TSR106548506(CH25H)
PGUT117673144(CH25H)
VKO123018246(CH25H)
PMUA114596710(CH25H)
ZVI118096366(CH25H)
GJA107121654(CH25H)
XLA100037239(ch25h.L) 108697353(ch25h.S) 108710600
XTR100494906(ch25h) 100497262 100497425
NPR108788867 108788868 108788869 108796192 108796200 108804259
RTEM120930304 120931550 120947102 120947103
BBUF120985595 120986151 121003961 121003962
BGAR122925766 122929725 122940819(CH25H)
DRE100005337 100136851(ch25hl2) 336673(ch25hl1.1) 494109(ch25h) 798384(ch25hl1.2)
SRX107715196 107718661 107720887 107725732 107725743 107736627 107740202 107753436 107757531 107758440
SANH107655924 107663027 107669234 107683723 107690388 107691130 107691148 107700893 107701946
SGH107562118 107567886 107567893 107584143 107584572 107585461 107594211 107596167 107597898
CCAR109053824 109056552 109056804 109062966 109075736 109082232 109099486 109099495 109109020 109109516
CAUA113049542 113056214 113056215 113061636 113070454 113075320 113076202 113095476 113112232 113118497
IPU100528885(ch25h) 108255855(ch25hl3) 108278808(ch25hl2)
PHYP113527115(ch25h) 113535659 113536926
SMEO124380638(ch25hl3) 124389010(ch25h) 124397654(ch25hl2)
TFD113646574(ch25hl2) 113657139(ch25hl3) 113662513(ch25h)
AMEX103034249 103034469 103045179 103046335 103047082(ch25h) 111194838
EEE113576619 113579506(ch25h) 113581374 113583153
TRU101065133 101077007 101077849(ch25h)
TNGGSTEN00021570G001 GSTEN00025231G001
LCO104917835 104917877 104926621 104936550(ch25h) 109142690
NCC104944613 104946607 104946608 104954979
CGOB115009395 115010316 115014068 115017024(ch25h)
ELY117246843 117248764 117251940(ch25hl3) 117262752(ch25hl1.1) 117262835(ch25hl1.2) 117270128(ch25hl2)
PLEP121944851(ch25hl3) 121950428(ch25hl1.2) 121950544(ch25hl1.1) 121958812(ch25h) 121959425(ch25hl2)
SLUC116038559(ch25hl1.2) 116050499(ch25hl3) 116058002(ch25h) 116062991(ch25hl2)
ECRA117940092 117944316(ch25hl3) 117949050(ch25hl1.1) 117959182(ch25hl2)
PFLV114548621 114555330 114570545 114572561
GAT120809648(ch25hl1.1) 120812854 120819662(ch25h) 120830874 120831757(ch25hl2)
PPUG119212566(ch25h) 119225133(ch25hl3) 119226756(ch25hl2)
MSAM119892469 119893763(ch25hl1.1) 119894120(ch25hl1.2) 119896308 119904787 119914596(ch25hl2) 119915784(ch25h) 119916808
CUD121509691(ch25hl3) 121514558(ch25hl1.2) 121515688(ch25hl1.1) 121525603(ch25hl2) 121528501(ch25h)
MZE101464039 101464326 101470860 101481798 105941282 106674885 112429828
ONL100696013 100696562 100699268 100699809 100701652 109194213(ch25h)
OAU116315924(ch25hl2) 116322653(ch25hl3) 116324416(ch25h) 116326256(ch25hl1.1) 116326257(ch25hl1.2)
OLA101156734 101157212 101157865 101164274(ch25h) 101168135
OML112141841(ch25h) 112147991(ch25hl3) 112151827(ch25hl1.2) 112152648(ch25hl1.1) 112162812(ch25hl2)
XMA102220316 102220847 102226187(ch25h) 102228291 102233589
XCO114149338 114150608(ch25h) 114151933 114153140 114154193
XHE116711389 116724864 116726903(ch25h) 116730350 116732729
PRET103466608 103466611 103466612 103469762 103473293 103482136(ch25h)
PFOR103135849 103146713 103147873 103147874 103154699(ch25h)
PLAI106939549(ch25h) 106946133 106946155 106952272 106957700
PMEI106908836(ch25h) 106927449 106928281 106928292 106928736
GAF122822928(ch25h) 122828451(ch25hl3) 122835305(ch25hl1.2) 122835307(ch25hl1.1) 122847290(ch25hl2)
CVG107090365 107098555 107099843(ch25h) 107099912 107099916 107105109
CTUL119772477(ch25hl1.1) 119772481 119788292(ch25hl2) 119792881(ch25h) 119795570(ch25hl3)
GMU124859703 124872886(ch25hl2) 124875354(ch25h) 124878233(ch25hl3)
NFU107374896 107387499(ch25h) 107388554 107388555 107393522
KMR108229945(ch25hl1.1) 108230007(ch25hl1.2) 108234932(ch25hl3) 108244749(ch25hl2) 108250736(ch25h)
ALIM106514260 106514353 106527498 106528206(ch25h) 106535365
NWH119414095(ch25hl3) 119416436(ch25hl1.1) 119416438(ch25hl1.2) 119423881(ch25h) 119424452(ch25hl2)
AOCE111568530(ch25h) 111572649 111580157 111580177 111580718 111588232 111588578
CSEM103379449 103380340 103382303(ch25h) 103389923 103397457
POV109624069 109624296 109626203 109633719 109646054
SSEN122758601(ch25hl2) 122759451(ch25h) 122761719(ch25hl1.1) 122769910(ch25hl3) 122770032(ch25hl1.2)
HHIP117753470(ch25h) 117762572(ch25hl1.1) 117762574(ch25hl1.2) 117768407(ch25hl3) 117771696(ch25hl2)
LCF108873757 108875282 108875283 108878399 108891073 108891484(ch25h) 108895464 108897287
SDU111220117(ch25h) 111228628 111228688 111231653 111235179 111239585
SLAL111647921 111657183(ch25h) 111666783 111667029 111667050 111670054
XGL120792869(ch25hl1.2) 120793386(ch25hl1.1) 120793946(ch25h) 120796129 120805429(ch25hl3) 120806724(ch25hl2)
HCQ109524973 109529265 109531322
BPEC110156548 110156554 110157477 110161056
MALB109951790 109954096 109954943 109960357 109966367 109969654(ch25h)
SASA100195833(c25ha) 106561197 106563107 106567920 106577089 106579045 106580502 106585111 106589227
OTW112218585 112219235(ch25hl1.2) 112223449(ch25h) 112223989 112223991 112230551 112248656 112253351 112253430 112262684
OMY110491381(ch25h) 110495809 110498990 110498991 110509673 110522829 110525871 110529015 110532186 110536255
OGO123992595 124006600(ch25hl2) 124033247 124036489 124036494(ch25hl1.1) 124039270 124039271 124040765 124043028(ch25hl1.2) 124048168(ch25h)
ONE115106155 115108916 115111060 115117321 115123412 115134240 115136334 115140690 115144560 115144561
SALP111952029 111953068 111957758 111960989 111962419 111963625 111967775 111973817 111978295
SNH120017986 120021879 120029730 120029734 120029768 120029796 120045437(ch25h) 120053228 120062780 120065844(ch25hl1.2) 120066274
ELS105006363 105014364 105015270 114839368 114839369
SFM108925090 108925091 108931682 108939583(ch25h) 108940315
PKI111848549 111849940 111849941 111853761(ch25h)
AANG118213427(ch25hl2) 118215571(ch25hl1.2) 118221090(ch25h) 118222843(ch25hl1.1) 118237105(ch25hl3)
LOC102684791(ch25h) 102688217 102697999 102698265 102698466
PSPA121302508 121303173 121322034 121325861(ch25h) 121327316 121328677 121330015 121330075
ARUT117397904 117403758 117403820 117403828 117416266 117418550 117966132 117973423
LCM102349366 102354597 102355462 102359996(CH25H) 102362070
CMK103180777(ch25h) 103186385(ch25hl2) 103188202(ch25hl1.1)
RTP109929454(ch25h) 109932007 109934755
BFO118403424 118404076
BBEL109463145 109474254
CIN104266587
SCLV120345747 120348064
SPU583592
SKO100368773 102803507
CELCELE_F35C8.5(F35C8.5)
CBRCBG_14675
LGILOTGIDRAFT_103020 LOTGIDRAFT_194826
PCAN112554126 112574477
BGT106054271 106054281
GAE121375390 121378554
HRF124146820 124147958
HRJ124260256 124290972
CRG105321530 105336654
MYI110447233 110455884
PMAX117325553 117334195
MMER123522906 123523342
LAK106158069 106178152
ADF107342391
AMIL114976650
CIMCIMG_09639
CPWCPC735_023820
AALTCC77DRAFT_1062674
 » show all
Reference
1  [PMID:9852097]
  Authors
Lund EG, Kerr TA, Sakai J, Li WP, Russell DW
  Title
cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism.
  Journal
J Biol Chem 273:34316-27 (1998)
DOI:10.1074/jbc.273.51.34316
  Sequence
[hsa:9023] [mmu:12642]
Reference
2  [PMID:11967195]
  Authors
Chen JJ, Lukyanenko Y, Hutson JC.
  Title
25-hydroxycholesterol is produced by testicular macrophages during the early postnatal period and influences differentiation of Leydig cells in vitro.
  Journal
Biol Reprod 66:1336-41 (2002)
DOI:10.1095/biolreprod66.5.1336
  Sequence
[rno:309527]
Reference
3  [PMID:12390873]
  Authors
Lukyanenko Y, Chen JJ, Hutson JC.
  Title
Testosterone regulates 25-hydroxycholesterol production in testicular macrophages.
  Journal
Biol Reprod 67:1435-8 (2002)
DOI:10.1095/biolreprod.102.007575
  Sequence
[rno:309527]
Reference
4  [PMID:7947683]
  Authors
Fox BG, Shanklin J, Ai J, Loehr TM, Sanders-Loehr J.
  Title
Resonance Raman evidence for an Fe-O-Fe center in stearoyl-ACP desaturase. Primary sequence identity with other diiron-oxo proteins.
  Journal
Biochemistry 33:12776-86 (1994)
DOI:10.1021/bi00209a008
Reference
5  [PMID:12543708]
  Authors
Russell DW
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu Rev Biochem 72:137-74 (2003)
DOI:10.1146/annurev.biochem.72.121801.161712
  Sequence
[mmu:12642]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.38
IUBMB Enzyme Nomenclature: 1.14.99.38
ExPASy - ENZYME nomenclature database: 1.14.99.38
BRENDA, the Enzyme Database: 1.14.99.38
CAS: 60202-07-5
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