KEGG   ENZYME: 1.14.99.66
Entry
EC 1.14.99.66               Enzyme                                 
Name
[histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase;
KDM1 (gene name);
LSD1 (gene name);
lysine-specific histone demethylase 1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
[histone H3]-N6,N6-dimethyl-L-lysine4:acceptor oxidoreductase (demethylating)
Reaction(IUBMB)
a [histone H3]-N6,N6-dimethyl-L-lysine4 + 2 acceptor + 2 H2O = a [histone H3]-L-lysine4 + 2 formaldehyde + 2 reduced acceptor (overall reaction) [RN:R12462];
(1a) a [histone H3]-N6,N6-dimethyl-L-lysine4 + acceptor + H2O = a [histone H3]-N6-methyl-L-lysine4 + formaldehyde + reduced acceptor [RN:R12463];
(1b) a [histone H3]-N6-methyl-L-lysine4 + acceptor + H2O = a [histone H3]-L-lysine4 + formaldehyde + reduced acceptor [RN:R12464]
Reaction(KEGG)
Substrate
[histone H3]-N6,N6-dimethyl-L-lysine4;
acceptor [CPD:C00028];
H2O [CPD:C00001];
[histone H3]-N6-methyl-L-lysine4
Product
[histone H3]-L-lysine4;
formaldehyde [CPD:C00067];
reduced acceptor [CPD:C00030];
[histone H3]-N6-methyl-L-lysine4
Comment
The enzyme specifically removes methyl groups from mono- and dimethylated lysine4 of histone 3. During the reaction the substrate is oxidized by the FAD cofactor of the enzyme to generate the corresponding imine, which is subsequently hydrolysed in the form of formaldehyde.The enzyme is similar to flavin amine oxidases, and differs from all other known histone lysine demethylases, which are iron(II)- and 2-oxoglutarate-dependent dioxygenases. The physiological electron acceptor is not known with certainty. In vitro the enzyme can use oxygen, which is reduced to hydrogen peroxide, but generation of hydrogen peroxide in the chromatin environment is unlikely as it will result in oxidative damage of DNA.
History
EC 1.14.99.66 created 2019
Orthology
K11450  [histone H3]-N6,N6-dimethyl-L-lysine4 FAD-dependent demethylase
Genes
HSA23028(KDM1A)
PTR456614(KDM1A)
PPS100995039(KDM1A)
GGO101144258(KDM1A)
PON100171870(KDM1A)
NLE100579631(KDM1A)
HMH116813055(KDM1A)
MCC718609(KDM1A)
MCF102135743(KDM1A)
MTHB126954770
MNI105494413(KDM1A)
CSAB103225404(KDM1A)
CATY105595010(KDM1A)
PANU101010766(KDM1A)
TGE112621190(KDM1A)
MLEU105529437(KDM1A)
RRO104663232(KDM1A)
RBB108538436(KDM1A)
TFN117093150(KDM1A)
PTEH111547709(KDM1A)
CANG105514283(KDM1A)
CJC100406478(KDM1A) 103787766
SBQ101034653(KDM1A)
CIMI108308628(KDM1A)
CSYR103270779(KDM1A)
MMUR105863495(KDM1A)
LCAT123634438(KDM1A)
PCOQ105815693(KDM1A)
OGA100948050(KDM1A)
MMU99982(Kdm1a)
MCAL110292704(Kdm1a)
MPAH110322979(Kdm1a)
RNO500569(Kdm1a)
MCOC116096867(Kdm1a)
ANU117708797(Kdm1a)
MUN110545399(Kdm1a)
CGE100770721(Kdm1a)
MAUA101823674(Kdm1a)
PROB127213383(Kdm1a)
PLEU114697214(Kdm1a)
MORG121448564(Kdm1a)
MFOT126506926
AAMP119816860(Kdm1a)
NGI103746403(Kdm1a)
HGL101706376(Kdm1a)
CPOC100723459(Kdm1a)
CCAN109681855(Kdm1a)
DORD105997903(Kdm1a)
DSP122115260(Kdm1a)
PLOP125354700(Kdm1a)
NCAR124990146
MMMA107142145(Kdm1a)
OCU100358443
OPI101536443(KDM1A)
TUP102491246(KDM1A)
GVR103590545(KDM1A)
CFA478193(KDM1A)
CLUD112660368(KDM1A)
VVP112930162(KDM1A)
VLG121496707(KDM1A)
NPO129513311(KDM1A)
AML100469590(KDM1A)
UMR103666569(KDM1A)
UAH113268534(KDM1A)
UAR123782267(KDM1A)
ELK111154639
LLV125096779
MPUF101680728(KDM1A)
MNP132001752(KDM1A)
MLK131809011(KDM1A)
NVS122899554(KDM1A)
ORO101371349(KDM1A)
EJU114199057(KDM1A)
ZCA113908763(KDM1A)
MLX118019904(KDM1A)
NSU110575168(KDM1A)
LWW102744436(KDM1A)
FCA101096583(KDM1A)
PYU121022945(KDM1A)
PCOO112870421(KDM1A)
PBG122480080(KDM1A)
PVIV125172726(KDM1A)
LRUF124515788
PTG102952813(KDM1A)
PPAD109274289(KDM1A)
PUC125913718
AJU106977650
HHV120231390(KDM1A)
BTA532997(KDM1A)
BOM102287646(KDM1A)
BIU109574017(KDM1A)
BBUB102396562(KDM1A)
BBIS104981096(KDM1A)
CHX102185879(KDM1A)
OAS101121409(KDM1A)
BTAX128043394(KDM1A)
ODA120855026(KDM1A)
CCAD122426548(KDM1A)
MBEZ129538204(KDM1A) 129559913
SSC100127157(KDM1A)
CFR102517288(KDM1A)
CBAI105070864(KDM1A)
CDK105088880(KDM1A)
VPC102533289(KDM1A)
BACU103012479(KDM1A)
BMUS118905801(KDM1A)
LVE103084888(KDM1A)
OOR101273117(KDM1A)
DLE111163462(KDM1A)
PCAD102995575(KDM1A)
PSIU116748436(KDM1A)
NASI112414418(KDM1A)
ECB100071646(KDM1A)
EPZ103551290(KDM1A)
EAI106839994(KDM1A)
MYB102261213(KDM1A)
MYD102754604(KDM1A)
MMYO118675325(KDM1A)
MLF102441324(KDM1A)
PKL118723786(KDM1A)
EFUS103291963(KDM1A)
MNA107538326(KDM1A)
DRO112299203(KDM1A)
SHON118977833(KDM1A)
AJM119042278(KDM1A)
PDIC114497837(KDM1A)
PHAS123832048(KDM1A)
MMF118626407(KDM1A)
PPAM129069159(KDM1A)
HAI109375888(KDM1A)
RFQ117027824(KDM1A)
PALE102887129(KDM1A)
PGIG120596321(KDM1A)
PVP105290441(KDM1A)
RAY107513066(KDM1A)
MJV108391096(KDM1A)
TOD119235233(KDM1A)
SARA101542116(KDM1A)
SETR126011026(KDM1A)
LAV100663912(KDM1A)
TMU101347919
ETF101645404(KDM1A)
DNM101420988(KDM1A)
MDO100025161(KDM1A)
GAS123241010(KDM1A)
SHR100923421(KDM1A)
AFZ127555729
PCW110206041(KDM1A)
OAA100086658(KDM1A)
GGA419571(KDM1A)
PCOC116230114(KDM1A)
MGP100544521(KDM1A)
CJO107323904(KDM1A)
TPAI128088523(KDM1A)
LMUT125703890(KDM1A)
NMEL110387355(KDM1A)
APLA101803659(KDM1A)
ACYG106046571(KDM1A)
CATA118257303(KDM1A)
AFUL116497985(KDM1A)
TGU100221744(KDM1A)
LSR110484269(KDM1A)
SCAN103822578(KDM1A)
PMOA120511678(KDM1A)
OTC121342723(KDM1A)
PRUF121359874(KDM1A)
GFR102041827(KDM1A)
FAB101810697(KDM1A)
OMA130262093(KDM1A)
PHI102109140(KDM1A)
PMAJ107214056(KDM1A)
CCAE111939020(KDM1A)
CCW104697964(KDM1A)
CBRC103614642(KDM1A)
ETL114061578(KDM1A)
ZAB102070080(KDM1A)
ZLE135457319(KDM1A)
ACHL103808196(KDM1A)
SVG106856107(KDM1A)
MMEA130581882(KDM1A)
HRT120762996(KDM1A)
FPG101923221(KDM1A)
FCH102058829(KDM1A)
CLV102088529(KDM1A)
EGZ104132548(KDM1A)
NNI104015516(KDM1A)
PCRI104024412(KDM1A)
PLET104620970
EHS104513426(KDM1A)
PCAO104041452(KDM1A)
ACUN113488263(KDM1A)
TALA104366984(KDM1A)
PADL103925455(KDM1A)
AFOR103905072(KDM1A)
ACHC115351984(KDM1A)
HALD104311330(KDM1A)
HLE104837246(KDM1A)
AGEN126047464
GCL127025006
CSTI104554150(KDM1A)
CMAC104487132(KDM1A)
MUI104533808(KDM1A)
BREG104633717(KDM1A)
GSTE104260759(KDM1A)
MNB103780978(KDM1A)
OHA104328064(KDM1A)
SHAB115615991 115616143(KDM1A)
DPUB104310069(KDM1A)
CPEA104393407(KDM1A)
CVF104293484(KDM1A)
RTD128918764(KDM1A)
CUCA104061495(KDM1A)
BRHI104495457(KDM1A)
AAM106484726(KDM1A)
AROW112971154(KDM1A)
NPD112951862(KDM1A)
TGT104571756(KDM1A)
DNE112984542(KDM1A)
SCAM104152038(KDM1A)
ASN102375236(KDM1A)
AMJ102563192(KDM1A)
CPOO109318445(KDM1A)
GGN109294012(KDM1A)
PSS102460305(KDM1A)
CMY102931629(KDM1A)
CCAY125624714(KDM1A)
DCC119845333(KDM1A)
CPIC101940420(KDM1A)
TST117868370(KDM1A)
CABI116826277(KDM1A)
MRV120389133(KDM1A)
ACS100557968(kdm1a)
ASAO132780315(KDM1A)
PVT110089669(KDM1A)
SUND121915735(KDM1A)
PBI103067389(KDM1A)
PMUR107288027(KDM1A)
CTIG120300147(KDM1A)
TSR106540788(KDM1A)
PGUT117669298(KDM1A)
APRI131204476(KDM1A)
PTEX113437434(KDM1A)
NSS113419875(KDM1A)
VKO123026095(KDM1A)
PMUA114603591(KDM1A)
PRAF128418603(KDM1A)
ZVI118087744(KDM1A)
HCG128332557(KDM1A)
GJA107116662(KDM1A)
STOW125434929(KDM1A)
EMC129343459(KDM1A)
XLA100127344(kdm1a.S) 108708259(kdm1a.L)
XTR100216250(kdm1a)
NPR108788279(KDM1A)
RTEM120926857(KDM1A)
BBUF120995362(KDM1A)
BGAR122932490(KDM1A)
MUO115480881(KDM1A)
GSH117365042(KDM1A)
DRE558450(kdm1a)
SRX107711588
SANH107694865 107697707
SGH107582487
CCAR109048821(kdm1a)
CAUA113098446 113117536
CGIB127933332 127976370
PTET122362007(kdm1a)
LROH127179663(kdm1a)
OMC131523222(kdm1a)
PPRM120484808(kdm1a)
RKG130069919(kdm1a)
MAMB125269126(kdm1a)
CIDE127498673
MASI127411423 127455097
TROS130560314(kdm1a)
TDW130437274(kdm1a)
MANU129417475(kdm1a)
IPU108269802
IFU128612980(kdm1a)
PHYP113533951(kdm1a)
SMEO124389936(kdm1a)
TFD113651181(kdm1a)
TVC132852894(kdm1a)
TRN134325016(kdm1a)
AMEX103023119(kdm1a)
CMAO118823321(kdm1a)
EEE113584671(kdm1a)
CHAR105908309(kdm1a)
TRU101073663(kdm1a)
TFS130513074(kdm1a)
TNGGSTEN00032051G001
LCO104931691(kdm1a)
NCC104968042(kdm1a)
TBEN117495248(kdm1a)
CGOB115027390(kdm1a)
PGEO117467647(kdm1a)
GACU117556396(kdm1a)
EMAC134881900(kdm1a)
ELY117267293(kdm1a)
EFO125900681(kdm1a)
PLEP121953332(kdm1a)
SLUC116057633(kdm1a)
ECRA117935764(kdm1a)
ESP116670128(kdm1a)
PFLV114546575(kdm1a)
GAT120833360(kdm1a)
PPUG119227607(kdm1a)
AFB129103899(kdm1a)
CLUM117725111(kdm1a)
PSWI130201167(kdm1a)
MSAM119909920(kdm1a)
SCHU122861745(kdm1a)
CUD121526479(kdm1a)
ALAT119004662(kdm1a)
MZE101466759(kdm1a)
ONL100702505(kdm1a)
OAU116327060(kdm1a)
OLA101172293(kdm1a)
OML112143592(kdm1a)
CSAI133462307(kdm1a)
XMA102231844(kdm1a)
XCO114134181(kdm1a)
XHE116708968(kdm1a)
PRET103459946(kdm1a)
PFOR103147265(kdm1a)
PLAI106936795(kdm1a)
PMEI106928434(kdm1a)
GAF122845663(kdm1a)
PPRL129374705(kdm1a)
CVG107084713(kdm1a)
CTUL119797817(kdm1a)
GMU124855435(kdm1a)
NFU107392201(kdm1a)
KMR108236889(kdm1a)
ALIM106533898
NWH119427524(kdm1a)
AOCE111573331(kdm1a)
MCEP125023899(kdm1a)
CSEM103378492(kdm1a)
POV109636394(kdm1a)
SSEN122782955(kdm1a)
HHIP117756634(kdm1a)
HSP118116543(kdm1a)
PPLT128451082(kdm1a)
SMAU118286182(kdm1a)
LCF108874888
SDU111226160(kdm1a)
SLAL111655910(kdm1a)
XGL120795666(kdm1a)
HCQ109526830(kdm1a)
SSCV125980471
SBIA133513339(kdm1a)
PEE133412950(kdm1a)
PTAO133488368(kdm1a)
BPEC110161360(kdm1a)
MALB109955009(kdm1a)
BSPL114848220(kdm1a)
SJO128375634(kdm1a)
SASA106562352 106610674
STRU115162572 115172875
OTW112256467 112262062
OMY110498149(kdm1a) 110505285
OGO124001588 124046269
ONE115138296 115146423
OKI109894674 109903851
OKE118362272 118368525
SALP111968924 112068085
SNH120023537(kdm1a) 120060168
CCLU121538976 121545121
ELS105016064(kdm1a)
SFM108922806(kdm1a)
PKI111854724(kdm1a)
AANG118235440(kdm1a)
LOC102685391(kdm1a)
PSPA121308237(kdm1a)
ARUT117413319 117414103 117962911
PSEX120517387(kdm1a)
LCM102363978(KDM1A)
CMK103179609(kdm1a) 121849774
RTP109926736(kdm1a)
CPLA122563535(kdm1a)
HOC132829856(kdm1a)
LERI129709901(kdm1a)
PMRN116952908(KDM1A)
LRJ133353612(KDM1A)
BFO118422441
BBEL109472794
CIN100187100
SCLV120344990
SPU575875
LPIC129259185
APLC110990427
AJC117116761
SKO100373836
DMEDmel_CG17149(Su(var)3-3)
DER6545751
DSE6616257
DSIDsimw501_GD14861(Dsim_GD14861)
DYADyak_GE19651
DAN6507938
DSR110178555
DPO117185142 4812867
DPE6596031
DMN108152784
DWI6638853
DGR6557229
DMODmoj_GI11530
DAZ108613120
DNV108651092
DHE111600951
DVI6624613
CCAT101461855
BOD106618034
BDR105227333
RZE108378801
AOQ129241522
TDA119672920 119676302
MDE101897898
SCAC106094569
LCQ111681191
LSQ119609704
GFS119632213
ECOE129951994
CLON129613583
HIS119650175
AGA1280978
ACOZ120955630
AARA120900208
AMER121598493
ASTE118505889 118516469
AFUN125767394
AMOU128309407
AALI118466258
AAG5574767
AALB109422398
ASUA134220950
CQUCpipJ_CPIJ002197
CPII120417587
CNS116341632
BCOO119071155
AME726469
ACER108001570
ALAB122710417
ADR102677004
AFLR100868785
BIM100740140
BBIF117205179
BVK117243561
BVAN117164701
BTER100647583
BAFF126928919
BPYO122572094
BPAS132909181
FVI122533504
CCAL108622958
OBB114871968
OLG117605887
MGEN117220295
NMEA116426390
CGIG122401075
SOC105200954
MPHA105832792
AEC105143630
ACEP105622905
PBAR105433548
VEM105564973
HST105185849
DQU106751689
CFO105253997
FEX115234610
LHU105674077
PGC109854993
OBO105282940
PCF106786794
PFUC122522128
VPS122638115
VCRB124426245
VVE124946949
NVI100678665
CSOL105360759
TPRE106658470
LHT122510611
LBD127276611
MDL103580293
CGLO123271527
FAS105263211
DAM107041294
AGIF122855020
CINS118065648
VCAN122405851
CCIN107267660
DSM124409338
NPT124218113
NFB124181502
NLO107220624
NVG124303377
AROA105692451
TCA103315031
DPA109547044
SOY115879099
AGRG126748190
ATD109597000
CSET123316124
AGB108905798
LDC111506347
DVV114331553
NVL108560657
APLN108735938
PPYR116169590
OTU111420804
BMOR101739058
BMAN114240311
MSEX115445640
DPLKGM_203173
BANY112057943
MJU123870247
NIQ126772486
VCD124543931
MCIX123658188
PMAC106719592
PPOT106103126
PXU106122099
PRAP110998007
PBX123713842
PNAP125063026
ZCE119833759
CCRC123696832
LSIN126972211
AAGE121733849
HAW110370308(Su(var)3-3)
HZE124633665
TNL113497903
SLIU111357637
OFU114361070
ATRA106136234
PXY105386718(bursa)
PGW126371216
LGLY125229312
CFEL113366183
CCRN123296727 123301095
API100168654
DNX107174085
AGS114126851
RMD113549832
ACOO126836037
DVT126910446
DCI103512113
CLEC106672331
HHAL106677548
NLU111059681
MQU128985742
PHUPhum_PHUM106950
FOC113204568 113207403
TPAL117652223
ZNE110831592 110841493
CSEC111864370 111865515
SGRE126284166
BROR134541268 134541884
IEL124165642
FCD110853963
DPXDAPPUDRAFT_39808
DMK116935472
DPZ124343487
PVM113807228
PJA122262527
PCHN125032108
PMOO119596469
HAME121869618
PCLA123769852
CQD128699780
PTRU123501269
ESN127005220
MNZ135226955
HAZT108664332
EAF111695147
LSM121115894
TCF131879015
PPOI119104122
ISC120842250 8029709
DSV119442251 119466038
RSAN119371688 119377057
RMP119159651 119163729
VDE111244720 111245465
VJA111261470 111271582
TUT107368558
DPTE113789474 113795938
DFR124492606 124500459
CSCU111638896
PTEP107446922
ABRU129972669
UDV129234543
SDM118182146
LPOL106464174
PMEO129587433 129587466
HROHELRODRAFT_90753
LGILOTGIDRAFT_154476
PVUL126810979
PCAN112561001
BGT106057596
GAE121369986 121391193
HRF124133544
HRJ124279824
CRG105346267
CVN111103635 111109649
CANU128184668
OED125675105
MYI110454266
PMAX117328302
MCAF127724654
MMER123563686 128549072
RPHI132743205
DPOL127865834
OBI106869849
OSN115224219
LJP135479956
LAK106170923
SMMSmp_150560
SHXMS3_00009234(KDM1A_1)
OVIT265_01693
EGLEGR_08887
LLON135488369
NVE5512813
EPA110237306
ATEN116302459
ADF107335615
AMIL114968998
PDAM113675980
SPIS111342045
DGT114533681
XEN124441854
HMG105844871
HSY130645197
TADTRIADDRAFT_34137
AQU100631436
OSA4344644
DOSAOs08t0143400-01(Os08g0143400)
OGL127781674
BDI100835742
ATS109781192
TDC119330479 119338613
TAES123147418 123157372 123165349
TUA125519949
LPER127314487
LRD124683171
PVIR120678932 120711365
PDA103724047
EGU105045418
MUS103986351
DCT110107040
PEQ110039551
AOF109844854
AVMJQX13_23305
 » show all
Reference
1  [PMID:15811342]
  Authors
Forneris F, Binda C, Vanoni MA, Mattevi A, Battaglioli E
  Title
Histone demethylation catalysed by LSD1 is a flavin-dependent oxidative process.
  Journal
FEBS Lett 579:2203-7 (2005)
DOI:10.1016/j.febslet.2005.03.015
  Sequence
[hsa:23028]
Reference
2  [PMID:19624733]
  Authors
Forneris F, Battaglioli E, Mattevi A, Binda C
  Title
New roles of flavoproteins in molecular cell biology: histone demethylase LSD1 and chromatin.
  Journal
FEBS J 276:4304-12 (2009)
DOI:10.1111/j.1742-4658.2009.07142.x
  Sequence
[hsa:23028]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.66
IUBMB Enzyme Nomenclature: 1.14.99.66
ExPASy - ENZYME nomenclature database: 1.14.99.66
BRENDA, the Enzyme Database: 1.14.99.66
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