KEGG ENZYME: 1.21.99.5

ENZYME: 1.21.99.5

Entry

EC 1.21.99.5                Enzyme

Name

tetrachloroethene reductive dehalogenase;
tetrachloroethene reductase

Class

Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With unknown physiological acceptors

Sysname

acceptor:trichloroethene oxidoreductase (chlorinating)

Reaction(IUBMB)

trichloroethene + chloride + acceptor = tetrachloroethene + reduced acceptor [RN:R05753]

Reaction(KEGG)

R05753;
(other) R05499 R05500 R05501

Substrate

trichloroethene [CPD:C06790];
chloride [CPD:C00698];
acceptor [CPD:C00028]

Product

tetrachloroethene [CPD:C06789];
reduced acceptor [CPD:C00030]

Comment

This enzyme allows the common pollutant tetrachloroethene to support bacterial growth and is responsible for disposal of a number of chlorinated hydrocarbons. The reaction occurs in the reverse direction. The enzyme also reduces trichloroethene to dichloroethene. Although the physiological reductant is unknown, the supply of reductant in some organisms involves menaquinol, which is reduced by molecular hydrogen via the action of EC 1.12.5.1, hydrogen:quinone oxidoreductase. The enzyme contains a corrinoid and two iron-sulfur clusters. Methyl viologen can act as electron donor in vitro.

History

EC 1.21.99.5 created 2001 as EC 1.97.1.8, transferred 2017 to EC 1.21.99.5

Pathway

ec00625

Chloroalkane and chloroalkene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K21647

tetrachloroethene reductive dehalogenase

Genes

SMUL:

SMUL_1531(pceA)

SHAL:

SHALO_1495

SULS:

Sdiek1_1587

SULJ:

SJPD1_1504

SULT:

FA592_00015 FA592_00070 FA592_13750

PBIZ:

LWC08_09670

GBX:	SANA_26110

CALK:

HUE98_04865

DETH:

HX448_00270 HX448_00330 HX448_01225 HX448_02085 HX448_02090 HX448_02095 HX448_03030 HX448_03180 HX448_03295 HX448_03300 HX448_03310 HX448_03315 HX448_03350 HX448_03355 HX448_05565 HX448_07555 HX448_07630 HX448_07635 HX448_08110 HX448_08115 HX448_08120 HX448_08405 HX448_08815 HX448_08840 HX448_08850 HX448_09535

DLF:	V8247_02575 V8247_05395

Reference

Authors

Holliger C Wohlfarth G, Diekert G.

Title

Reductive dechlorination in the energy metabolism of anaerobic bacteria.

Journal

FEMS Microbiol Rev 22:383-398 (1998)

Reference

Authors

Glod G, Angst W, Holliger C, Schwarzenbach RP.

Title

Corrinoid-mediated reduction of tetrachloroethene, trichloroethene, and trichlorofluoroethene in homogeneous aqueous solution: Reaction kinetics and reaction mechanisms.

Journal

Environ Sci Technol 31:253-260 (1997)

Reference

3 [PMID:8663199]

Authors

Neumann A, Wohlfarth G, Diekert G.

Title

Purification and characterization of tetrachloroethene reductive dehalogenase from Dehalospirillum multivorans.

Journal

J Biol Chem 271:16515-9 (1996)
DOI:10.1074/jbc.271.28.16515

Reference

4 [PMID:9224702]

Authors

Schumacher W, Holliger C, Zehnder AJ, Hagen WR.

Title

Redox chemistry of cobalamin and iron-sulfur cofactors in the tetrachloroethene reductase of Dehalobacter restrictus.

Journal

FEBS Lett 409:421-5 (1997)
DOI:10.1016/S0014-5793(97)00520-6

Reference

5 [PMID:8636034]

Authors

Schumacher W, Holliger C.

Title

The proton/electron ration of the menaquinone-dependent electron transport from dihydrogen to tetrachloroethene in "Dehalobacter restrictus".

Journal

J Bacteriol 178:2328-33 (1996)
DOI:10.1128/JB.178.8.2328-2333.1996

Other DBs

ExplorEnz - The Enzyme Database:

1.21.99.5

IUBMB Enzyme Nomenclature:

1.21.99.5

ExPASy - ENZYME nomenclature database:

1.21.99.5

BRENDA, the Enzyme Database:

1.21.99.5

CAS:	163913-51-7

LinkDB

DBGET integrated database retrieval system