KEGG ENZYME: 1.23.1.1

ENZYME: 1.23.1.1

Entry

EC 1.23.1.1                 Enzyme

Name

(+)-pinoresinol reductase;
pinoresinol/lariciresinol reductase;
pinoresinol-lariciresinol reductases;
(+)-pinoresinol/(+)-lariciresinol;
(+)-pinoresinol-(+)-lariciresinol reductase;
PLR

Class

Oxidoreductases;
Reducing C-O-C group as acceptor;
With NADH or NADPH as donor

Sysname

(+)-lariciresinol:NADP+ oxidoreductase

Reaction(IUBMB)

(+)-lariciresinol + NADP+ = (+)-pinoresinol + NADPH + H+ [RN:R10225]

Reaction(KEGG)

R10225

Substrate

(+)-lariciresinol [CPD:C10646];
NADP+ [CPD:C00006]

Product

(+)-pinoresinol [CPD:C05366];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

The reaction is catalysed in vivo in the opposite direction to that shown. A multifunctional enzyme that further reduces the product to the lignan (-)-secoisolariciresinol [EC 1.23.1.2, (+)-lariciresinol reductase]. Isolated from the plants Forsythia intermedia [1,2], Thuja plicata (western red cedar) [3], Linum perenne (perennial flax) [5] and Linum corymbulosum [6]. The 4-pro-R hydrogen of NADH is transferred to the 7-pro-R position of lariciresinol [1].

History

EC 1.23.1.1 created 2013

Pathway

ec00999

Biosynthesis of various plant secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K21568

pinoresinol/lariciresinol reductase

Genes

ATH:	AT1G32100(PRR1) AT4G13660(PRR2)

ALY:	9306436 9329761

CRB:	17878264 17898640 17899710

CSAT:

104719089 104723827 104723828 104732343 104741806 104757581 104757582 104777243

EUS:	EUTSA_v10008239mg EUTSA_v10009995mg EUTSA_v10026944mg

BRP:	103840191 103868905

BNA:	106441553 106451749 106451753 106453825 111203922 125608748

BOE:	106292815 106294294

RSZ:	108856438 108856439 108856515

THJ:	104798498 104814402 104814409

CPAP:

110826366

CIT:	102608004 102608287

CIC:	CICLE_v10001909mg CICLE_v10001913mg

PVY:	116120000 116125874 116126062 116137530 116141105

MINC:

123195988 123196053 123196855

TCC:	18606866 18606867

GRA:	105791865 105791869

GHI:	107900659 107900663 107929415 107929420

GAB:	108468897 108468899

HSYR:

120162653 120164192 120164207 120172355

DZI:	111286131 111289303 111304310

EGR:	104444249 104444250 104444251 104444254

RARG:

115731745 115744354 115757594

GMX:	100775382 100780465 100786578

GSJ:	114367117 114369002 114390881

PVU:	137808554 137811181 137811182

VRA:	106765640 106776909 106777876

VAR:	108331686 108335043 108335182

VUN:	114173139 114173523 114175607

VUM:

124823054

CCAJ:

109791501 109801295 109806363

APRC:

113858524 113869210 113869519

MTR:	11406505 11411102 25501556

TPRA:

123881501 123907611

CAM:	101492057 101501430

PSAT:

127074848 127119827 127119828

VVO:	131624483 131641724 131646643 131646645 131646647 131646648 131646649

LJA:	130711595 130740214 130740218

ADU:	107475254 107479134 107487050 107487131 107487132 127745668

AIP:	107622394 107624243 107641612 107642385 107642387

AHF:	112747483 112747484 112747485 112782427 112783546 112798030 112798048 112800088 112800089 112800090

LANG:

109332970 109349050

PCIN:

129299883 129299885 129310698 129311401 129312313 129322839

QSA:	O6P43_025080 O6P43_032233 O6P43_032234 O6P43_033394 O6P43_033395

FVE:	101307211 101307794 105351891

AANS:

126794693 126794822 126794823

RCN:	112177128 112177129 112177130

PPER:

18775767 18775835 18776245 18777823 18778052

PMUM:

103336794 103336795 103336796 103336797

PAVI:

110761041 110772979 110772980

PDUL:

117613517 117627184 117627185 117627188 117628069 117628098 117628169 117628285

MDM:	103453140 103453142

MSYL:

126596308 126596309

PXB:

103928035

PCOX:

137738124 137738707

ZJU:	107430985 107434370

MNT:	21405631 21405632 21405639

CSAV:

115715730 115715995 115715996 115716735

CSV:	101212644 101218862

CMO:	103499245 103499247

BHJ:	120077628 120078251

MCHA:

111022994 111025718

CMAX:

111466859 111466861 111466862

CMOS:

111464117 111464118 111464122

CPEP:

111776321 111776322 111811889

RCU:	8284632 8284633

MEAN:

126679873

JCU:

105632658

HBR:	110640204 110649727

MESC:

110602482 110604749 110605546

POP:	18094088 18094089 7465466

PEU:	105121482 105121485 105128209

PALZ:

118028664 118038662 118038664

PNZ:	133692775 133699112 133700753

JRE:	108980191 109014289

CILL:

122281553 122281556 122290381

CAVE:

132175489 132175736 132175890 132176272

AGLU:

133854309 133854381

QSU:	112001516 112001517 112001518 112015239 112023934 112023936 136069733 136069736

QLO:	115969769 115969770 115970900 115971845 115972164 115972599

TWL:	119993294 119997790 119997791 119997987

VVI:	100243193 100248319 100253437(IFRL1) 100259918 100260187

VRI:	117905205 117921037 117928281 117928296 117928956 117929200 117929210

SLY:	101258966 101259550

SPEN:

107021294 107021639

SOT:	102590543 102597564

SSTN:

125873254 125874732

SDUL:

129881689 129900339 129901055

CANN:

107869653 107872854 107872855

LBB:	132608820 132635391 132639992

NTA:	107767259 107806386 107825620

NSY:	104220290 104234527 138872096

NTO:	104092562 104092564 104102952

NAU:

109227228

INI:	109177381 109177391 109177392

ITR:	116001368 116001648 116002687 116002701

SIND:

105172735 105172736

OEU:	111387338 111387358 111387367 111408132 111408280

EGT:

105973797

SSPL:

121778590 121780365 121780799 121781020

SMIL:

131004047 131004049

SHIS:

125212402 125214001

APAN:

127255558

HAN:	110865009 110920952 110930107 110930108 110930109

ECAD:

122580001 122600817 122600894 122600922 122602236

LSV:	111887528 111898507 111902652

CCAV:

112529291 112529297 112529363 112529620

DCR:	108192976 108193011 108206746

CSIN:

114262347 114281967 114283576

RVL:

131333837

AEW:	130763691 130765593 130766469 130766914

DLT:	127786630 127786631 127786633 127786634

BVG:

104893088

SOE:

110801329

CQI:	110692822 110732670

ATRI:

130799740

MOF:

131164875

NNU:	104596321 104596322 104596323

MING:

122060488 122062137 122086245 122086283 122087666

TSS:	122642691 122664432 122664715

PSOM:

113322331

OSA:	4351925 4351926 4351932

DOSA:

Os12g0263200 Os12g0265100

OBR:	102717852 102718130

OGL:	127756218 127756250 127756441 127756791

BDI:

100842184

ATS:	109737033 109785841 109785845 109785846 120973419

TDC:	119332686 119337515 119353140 119353141 119353142 119360533 119362236 119362237 119362238 119362239 119362241

TAES:

123040504 123043537 123043538 123043539 123043540 123051402 123051405 123154222 123159917 123170562 123187358

TUA:	125523955 125535496 125535498

LPER:

127331363 127343531

LRD:	124646832 124647065 124649053 124675570 124675818

SBI:	110429729 110429730

ZMA:

103650598

MFLO:

136502557 136504171 136504520 136504577 136504849 136505247

SITA:

101764846 101781583

SVS:	117847104 117849214

PVIR:

120665987 120665988 120667037 120699226 120699228 120699229

PHAI:

112888068

PAUA:

133906517 133906598

PDA:	103697428 103698420

EGU:	105040244 105041891 105060630

MUS:

103981518

DCT:	110113106 110116470

PEQ:

110020110

AOF:	109827161 109840604

MSIN:

131218405 131233580 131247951 131247952 131247953 131247954 131249377 131249385 131249392 131249402 131249409

ACAK:

137934347 137934448 137945751 137950295

NCOL:

116252548 116252549 116254772 116254791 116255118 116255193

ATR:	18423509 18443892 18443893 18443896

CJF:	131030747 131030757 131030758 131052716 131062760 131065630 131065642 131072143 131858038

SMO:	SELMODRAFT_122655 SELMODRAFT_172293 SELMODRAFT_230858 SELMODRAFT_233106 SELMODRAFT_414671 SELMODRAFT_414678 SELMODRAFT_438560 SELMODRAFT_74219 SELMODRAFT_74385 SELMODRAFT_74402

SSE:

Ssed_3603

» show all

Reference

1 [PMID:8262939]

Authors

Chu A, Dinkova A, Davin LB, Bedgar DL, Lewis NG

Title

Stereospecificity of (+)-pinoresinol and (+)-lariciresinol reductases from Forsythia intermedia.

Journal

J Biol Chem 268:27026-33 (1993)

Reference

2 [PMID:8910615]

Authors

Dinkova-Kostova AT, Gang DR, Davin LB, Bedgar DL, Chu A, Lewis NG

Title

(+)-Pinoresinol/(+)-lariciresinol reductase from Forsythia intermedia. Protein purification, cDNA cloning, heterologous expression and comparison to isoflavone reductase.

Journal

J Biol Chem 271:29473-82 (1996)
DOI:10.1074/jbc.271.46.29473

Sequence

[ag:AAC49608]

Reference

3 [PMID:9872995]

Authors

Fujita M, Gang DR, Davin LB, Lewis NG

Title

Recombinant pinoresinol-lariciresinol reductases from western red cedar (Thuja plicata) catalyze opposite enantiospecific conversions.

Journal

J Biol Chem 274:618-27 (1999)
DOI:10.1074/jbc.274.2.618