KEGG   ENZYME: 1.4.9.1
Entry
EC 1.4.9.1                  Enzyme                                 
Name
methylamine dehydrogenase (amicyanin);
amine dehydrogenase;
primary-amine dehydrogenase;
amine: (acceptor) oxidoreductase (deaminating);
primary-amine:(acceptor) oxidoreductase (deaminating)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With a copper protein as acceptor
Sysname
methylamine:amicyanin oxidoreductase (deaminating)
Reaction(IUBMB)
methylamine + H2O + 2 amicyanin = formaldehyde + NH3 + 2 reduced amicyanin [RN:R00606]
Reaction(KEGG)
R00606
Substrate
methylamine [CPD:C00218];
H2O [CPD:C00001];
amicyanin [CPD:C19671]
Product
formaldehyde [CPD:C00067];
NH3 [CPD:C00014];
reduced amicyanin [CPD:C19672]
Comment
Contains tryptophan tryptophylquinone (TTQ) cofactor. The enzyme oxidizes aliphatic monoamines and diamines, histamine and ethanolamine, but not secondary and tertiary amines, quaternary ammonium salts or aromatic amines.
History
EC 1.4.9.1 created 1978 as EC 1.4.99.3, modified 1986, transferred 2011 to EC 1.4.98.1, transferred 2011 to EC 1.4.9.1
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K08685  quinohemoprotein amine dehydrogenase
K15228  methylamine dehydrogenase light chain
K15229  methylamine dehydrogenase heavy chain
Genes
YMO: HRD69_07500 HRD69_07515
CARU: P0E69_20625
VFU: vfu_A02015 vfu_A02018
VFL: AL536_13995 AL536_14010
PGH: FH974_17590 FH974_17605
PRE: PCA10_23420(mauB) PCA10_23450(mauA) PCA10_32510(peaA) PCA10_34250(mauB) PCA10_34280(mauA)
PFUW: KF707C_23300 KF707C_23330 KF707C_25120
POJ: PtoMrB4_25230
PLAL: FXN65_11130 FXN65_11145 FXN65_11785(peaA)
PCQ: PcP3B5_29870 PcP3B5_30140(aauA) PcP3B5_30170(aauB)
PMUI: G4G71_11895(peaA) G4G71_16135 G4G71_16150
PNT: G5B91_13190(peaA) G5B91_15900 G5B91_15915 G5B91_33160(peaA)
PPU: PP_5602(peaA)
PPF: Pput_2307
PPG: PputGB1_2221 PputGB1_2224 PputGB1_2475
PPW: PputW619_2945
PPT: PPS_2940
PPB: PPUBIRD1_2330
PPI: YSA_11298
PPX: T1E_1613
PPUH: B479_14560
PPUT: L483_18160
PPUN: PP4_23960(peaA)
PPUD: DW66_3200
PMON: X969_14050
PMOT: X970_13695
PPJ: RK21_01725
PMOL: CLJ08_00110(peaA)
PALD: LU682_012715(peaA)
PIX: RIN61_26575(peaA)
PMOS: O165_011585
PFL: PFL_4120(peaA)
PPRC: PFLCHA0_c41800
PPRO: PPC_4221(peaA)
PFO: Pfl01_2975
PMAN: OU5_1439
PEN: PSEEN2552 PSEEN2555 PSEEN2977
PDR: H681_11585
PSV: PVLB_12820
PKC: PKB_2656(mauA) PKB_2659(mauB) PKB_2912
PCH: EY04_20885
PCZ: PCL1606_20620
PCP: JM49_10330
PCHP: C4K32_4281
PALK: PSAKL28_24530
PSEM: TO66_21570
PSEC: CCOS191_2324
PSOS: POS17_4220(peaA)
PFK: PFAS1_02675
PSET: THL1_3122 THL1_3125 THL1_3144 THL1_3147 THL1_3176
PSIL: PMA3_20245
PYM: AK972_2150 AK972_2153
PKE: DLD99_19475(peaA)
PUM: HGP31_18355 HGP31_18370 HGP31_22220(peaA)
PMAO: PMYSY11_1047 PMYSY11_1050(mauA)
PSEP: C4K39_3038 C4K39_3041
PPII: QL104_15105 QL104_15120
PVW: HU752_028865(peaA)
PQI: KH389_12680(peaA)
PXN: HU772_009655 HU772_009670 HU772_012685(peaA)
PANH: HU763_013035(peaA)
PASG: KSS96_11100 KSS96_11115
PMAM: KSS90_11360(peaA)
PAZE: KSS91_08660(peaA)
PORY: EJA05_14485(peaA)
PTW: TUM18999_24310 TUM18999_32970 TUM18999_33000
PWY: HU734_013230(peaA)
PCAV: D3880_11325(peaA)
PIZ: LAB08_R20860 LAB08_R20890 LAB08_R42330(peaA)
PSIH: LOY51_14115(peaA)
PFIT: KJY40_20070(peaA)
PASI: LG197_20270(peaA)
PKM: PZ739_15150(peaA) PZ739_16400 PZ739_16415
PNB: NK667_05315(peaA)
PSOA: PSm6_45540 PSm6_50130 PSm6_50160
PTAI: ICN73_18625(peaA)
PKK: QQ992_12005(peaA)
PKJ: Q1W70_18750(peaA) Q1W70_25785 Q1W70_25800
PHOM: KJF94_17245(peaA)
PTRL: OU419_12830(peaA) OU419_13225 OU419_13240
PSJY: AA098_12455
PSHS: JJN09_10915(peaA)
PSNC: C0058_12620 C0058_12635
PFOU: OZ911_15435(peaA)
PBEJ: PSH84_16300(peaA)
PSKP: KU43P_23980 KU43P_30770 KU43P_30800
PDEU: QEP73_09025(peaA)
PSDT: NH234_15940 NH234_15955 NH234_20610(peaA)
PSWO: SD196_15255 SD196_15270 SD196_15935(peaA)
PMOR: PMm318_A29570 PMm318_A34250 PMm318_A34280
PMK: MDS_4213 MDS_4216
PKH: JLK41_14070(peaA)
PBB: AKN87_05350
PDEN: F1C79_04845(peaA) F1C79_07630 F1C79_07645
HARR: HV822_17320
PSPI: PS2015_1722
SPSH: FM037_08780 FM037_08795
ALTE: ALT761_03050 ALT761_03053
ALTR: BM528_03615
GAG: Glaag_4148 Glaag_4151
PMES: FX988_02772 FX988_02775
PAT: Patl_0157 Patl_0160
HMI: soil367_17600 soil367_17615
HARO: R50071_32560 R50071_32590
MNY: R50073_06010 R50073_44480 R50073_44510
KIM: G3T16_03325
CVB: R0135_13150 R0135_13165
CBRV: R0137_00990 R0137_01005
MMAF: GCM100_19830 GCM100_19860
MTHA: VSX76_15095(mauB) VSX76_15110(mauA)
GAI: IMCC3135_19700(aauA) IMCC3135_19715(aauB) IMCC3135_20060
HCS: FF32_04535
HAMS: NF683_05165(peaA)
HCAM: I4484_00320(peaA)
HAXH: J4377_16945(peaA)
HAFN: KF947_09345(peaA)
HVN: EI420_18585(peaA)
HNP: SR894_19505(peaA)
ALCA: ASALC70_04365(aauA) ASALC70_04368(aauB)
ADI: B5T_00161 B5T_00164 B5T_00533 B5T_00536
AXE: P40_02480 P40_02495
TWAN: HUF19_01030
MARS: A8C75_10935 A8C75_13875
MGEO: CFI10_12590(peaA) CFI10_12680(peaA)
MSEC: LN244_10005(peaA) LN244_10130(peaA)
MRZ: KDW95_04965(peaA)
NCU: F0U83_05970(peaA) F0U83_06020(peaA)
NJP: NEJAP_2487(peaA)
AJP: AMJAP_2431 AMJAP_2439
SUTR: L0B52_07765 L0B52_07780
PSE: NH8B_2195
CUH: BJN34_11000 BJN34_11020 BJN34_23755 BJN34_23770
REU: Reut_B4077 Reut_B4080
CBW: RR42_s0274 RR42_s0277
CCUP: BKK81_28180 BKK81_28195
CUP: BKK80_28180 BKK80_28195
CUU: BKK79_34095 BKK79_34110
BUR: Bcep18194_B0601 Bcep18194_B0604
BCM: Bcenmc03_5222 Bcenmc03_5225
BCH: Bcen2424_5059 Bcen2424_5062
BCJ: BCAM2301 BCAM2304
BCEN: DM39_4450(peaA)
BCEO: I35_6192 I35_6195
BAM: Bamb_4469 Bamb_4472
BAC: BamMC406_4994 BamMC406_4997
BMJ: BMULJ_04934 BMULJ_04937
BMU: Bmul_3580 Bmul_3583
BMK: DM80_4965 DM80_4968(aauA)
BMUL: NP80_4958(aauA) NP80_4961
BCT: GEM_3380 GEM_3383 GEM_5082
BCED: DM42_5686 DM42_5689(aauA)
BCEP: APZ15_27590 APZ15_27605
BDL: AK34_3489 AK34_3492(aauA)
BCON: NL30_01045 NL30_01060
BDF: WI26_17220
BTEI: WS51_08585 WS51_08600
BSEM: WJ12_31395 WJ12_31410
BPSL: WS57_02710 WS57_02725 WS57_10400
BMEC: WJ16_29835 WJ16_29850
BSTG: WT74_30205 WT74_30220
BSTL: BBJ41_25375 BBJ41_25390 BBJ41_34655 BBJ41_34670
BANN: JFN94_21045(peaA) JFN94_29355 JFN94_29370
BARI: NLX30_25745 NLX30_25760
BAEN: L3V59_26030 L3V59_26045 L3V59_33245(peaA)
BSER: R0290_18105 R0290_18120
BGF: BC1003_3869 BC1003_3872
BYI: BYI23_B006010 BYI23_B006040
BURK: DM992_22970 DM992_22985
BURT: BTHE68_33140 BTHE68_33170
BURM: GJG85_22575 GJG85_22590
BPH: Bphy_3809 Bphy_3812
BGE: BC1002_1587 BC1002_1590
BCAI: K788_0000141 K788_0000144
PHS: C2L64_30710 C2L64_30725
PTER: C2L65_27370 C2L65_27385
PMEG: FNZ07_14525 FNZ07_14540
PSAA: QEN71_14395 QEN71_14410
PPAI: E1956_39495(peaA) E1956_39820 E1956_39835
PLAD: PPGU16_43860 PPGU16_43890
PAZZ: PBP221_52550 PBP221_52580
BUO: BRPE64_BCDS07560 BRPE64_BCDS07590
CABK: NK8_36020 NK8_36050
BUE: BRPE67_BCDS08290 BRPE67_BCDS08320
AXY: AXYL_02123 AXYL_02126
AXO: NH44784_037061 NH44784_037091
AXN: AX27061_1937 AX27061_1940
AXX: ERS451415_01935(aauB) ERS451415_01938(aauA)
ADT: APT56_09285 APT56_09300
AIS: BUW96_00985 BUW96_01000
ASW: CVS48_14415 CVS48_14430
ACHR: C2U31_25675 C2U31_25690
ACHB: DVB37_08350 DVB37_08365
ADY: HLG70_12820 HLG70_12835
AAEG: RA224_22495 RA224_22510
ASEL: RAS12_12580 RAS12_12595 RAS12_16880(mauA) RAS12_16895
ACHA: KYT87_08865 KYT87_08880
AVH: WHX56_14835 WHX56_14850
ACHH: ABFG95_23850 ABFG95_23865
PUS: CKA81_14940 CKA81_14955
AKA: TKWG_18915 TKWG_18935
AMIM: MIM_c29740(mauA) MIM_c29770(mauB)
CDN: BN940_17491 BN940_17506
AAMM: FE795_01630 FE795_01645
ALCN: MID00_06280 MID00_06295
HPSE: HPF_21410
MPT: Mpe_A2653
METP: C1M51_14860 C1M51_14875
MFA: Mfla_0548 Mfla_0551
MMB: Mmol_1572 Mmol_1575
EBA: ebA2235(qhpA) ebA5178(qhpA)
ABRE: pbN1_04760(peaA)
APET: ToN1_01780(qhpA1) ToN1_38420(qhpA2)
DDEN: KI615_16540 KI615_16555
DECH: GBK02_00305 GBK02_00320
QAU: KI612_13825 KI612_13840
AZO: azo1239(qhpA) azo2257(mauA) azo2260(maub)
AOA: dqs_1354 dqs_2388 dqs_2391
AZA: AZKH_3023(qhpA)
AZI: AzCIB_2960
AZD: CDA09_16050(peaA)
AZR: CJ010_14330 CJ010_14345
TMZ: Tmz1t_0761 Tmz1t_2982
THU: AC731_014475 AC731_014490 AC731_017170
THAU: C4PIVTH_0800 C4PIVTH_1325(mauA) C4PIVTH_1328(mauB)
TCL: Tchl_2123
THK: CCZ27_00380
TAK: Tharo_1281
THAG: CKCBHOJB_03182
AZQ: G3580_00160(peaA)
MESP: C1M53_03980 C1M53_03995
KMN: HW532_09310(peaA)
RBS: RHODOSMS8_03492(aauA) RHODOSMS8_03495(aauB)
MEA: Mex_1p2770(mauB) Mex_1p2773(mauA)
MCH: Mchl_0562 Mchl_0565
METD: C0214_27025(mauA) C0214_27040(mauB)
HNI: W911_02545 W911_02560
FIL: BN1229_v1_3765(mauB) BN1229_v1_3768(mauA)
FIY: BN1229_v1_3755(mauB) BN1229_v1_3758(mauA)
PHL: KKY_2939 KKY_2942
PEM: OF122_03140 OF122_03155
PNF: V6617_03100 V6617_03115
DNP: N8A98_21260
BLAG: BLTE_16570
PLEO: OHA_1_02005(MadB) OHA_1_02008(MadA)
LAP: ACP90_10715
LABR: CHH27_09745(peaA)
LABP: FJ695_27525(peaA)
LABT: FIU93_16290
LAGG: B0E33_22635
CAK: Caul_0557 Caul_0560
LMD: METH_16640
LEJ: ETW24_23980(peaA)
LAQU: R2C4_20365(peaA)
LEV: ETW23_22245(peaA)
LCAE: K3721_19810(peaA)
CID: P73_3335 P73_3338
CEH: CEW89_12055 CEW89_12070
PALX: GQA70_00090(peaA)
PDE: Pden_1703 Pden_4730 Pden_4733
PAMI: JCM7686_0163 JCM7686_0166
PYE: A6J80_01745(peaA) A6J80_21995(mauB) A6J80_22010(mauA)
PCON: B0A89_14420
PZH: CX676_03775(peaA)
PARO: CUV01_13130(peaA)
PKD: F8A10_18985(peaA) F8A10_20020(mauA) F8A10_20035(mauB)
PPAN: ESD82_08405(peaA)
PSAN: HGN31_15410(peaA)
PMEH: JWJ88_20975(mauB) JWJ88_20990(mauA)
PSEW: JHW44_17740(peaA)
PALP: JHW40_00705(mauB) JHW40_00720(mauA)
PDIM: PAF18_02540 PAF18_02555
PFEO: E3U26_13605(mauA) E3U26_13620(mauB)
RHC: RGUI_4107
GFU: KM031_08500(peaA)
HML: HmaOT1_16775 HmaOT1_16790
PGV: SL003B_2489
THAS: C6Y53_00400(peaA)
RHP: LPB142_09660 LPB142_09675
HBA: Hbal_2737 Hbal_2741
NAR: Saro_1685 Saro_1688 Saro_3028 Saro_3818 Saro_3821
NPP: PP1Y_Mpl7173 PP1Y_Mpl7192
NOT: C7W88_08020(peaA) C7W88_11135 C7W88_11150 C7W88_11315 C7W88_11330
NOR: FA702_12465(peaA) FA702_12490 FA702_12505 FA702_14885 FA702_14900 FA702_20775 FA702_20790
NDR: HT578_04720(peaA) HT578_17915 HT578_17930
NHUM: PQ457_03230
NCP: U0041_07350 U0041_07365
NOVO: IM701_09270 IM701_09285 IM701_09420 IM701_09435 IM701_12710(peaA)
SMAG: AN936_02850 AN936_02865
SMAZ: LH19_18390 LH19_18405
SPJJ: V6U71_16220 V6U71_16235
SWI: Swit_3251 Swit_3254 Swit_4363 Swit_4365
SPHD: HY78_09285 HY78_09300
SPHM: G432_07310 G432_07325
SSAN: NX02_15530 NX02_15540 NX02_15555
SBIN: SBA_ch1_01450 SBA_ch1_01480 SBA_ch1_16750
SPHR: BSY17_2364(peaA) BSY17_2810(mauA) BSY17_2813
SHYD: CJD35_17800 CJD35_17815 CJD35_17865(peaA)
SYA: A6768_01720(peaA) A6768_17000 A6768_17015
SPAG: sphantq_01412(mauB) sphantq_01415(mauA) sphantq_02895
SPCA: GL174_07495(peaA) GL174_09035 GL174_09050
SPWT: N6H05_09395 N6H05_09410 N6H05_23340(peaA)
SMIC: SmB9_02170 SmB9_02200 SmB9_03800 SmB9_03830
ALB: AEB_P3055
ERQ: PS060_15585 PS060_15600
GFA: MKW11_12685(mauA) MKW11_12700
GDI: GDI0271(mauB) GDI0273(mauA) GDI1806(mauB) GDI1807(mauA)
GDJ: Gdia_0035 Gdia_0036 Gdia_2335 Gdia_2337
GXL: H845_1153 H845_1154 H845_1163 H845_1164 H845_1172 H845_1175
KEU: S101446_00287(mauB) S101446_00288(mauA) S101446_00300 S101446_00301 S101446_00310 S101446_00313
APT: APA01_04540 APA01_04570
APW: APA42C_04540 APA42C_04570
APF: APA03_04540 APA03_04570
APU: APA07_04540 APA07_04570
APG: APA12_04540 APA12_04570
APQ: APA22_04540 APA22_04570
APX: APA26_04540 APA26_04570
APZ: APA32_04540 APA32_04570
APK: APA386B_1936(mauB) APA386B_1939(mauA)
ASZ: ASN_2153(mauB) ASN_2156(mauA)
ASV: WG31_02710 WG31_02725
AACE: A0U92_10600 A0U92_10615
APER: A0U91_00065
APOM: CPF11_05025(mauA) CPF11_05040
ATO: CIW82_01900(mauA) CIW82_01915
AASC: A4S02_09860
ACET: DS739_02395 DS739_02410(mauA)
AOY: EOV40_002245 EOV40_002260(mauA)
NTN: D5366_10130 D5366_10145(mauA)
NGU: QN315_00185 QN315_00190 QN315_02220 QN315_02235 QN315_08615 QN315_08625
AZZ: DEW08_00570(peaA)
AZS: E6C72_23175(peaA)
NCB: C0V82_12865 C0V82_12880
MAGX: XM1_2531
ECOG: FIV45_14145(peaA) FIV45_16005(peaA)
GSO: PH603_12710 PH603_12725
ABU: Abu_0465
ABT: ABED_0440
ABL: A7H1H_0465
AELL: AELL_0619
ASUI: ASUIS_0583
AVP: AVENP_0707 AVENP_0712
ADZ: ADFLV_0614 ADFLV_1358
ARC: ABLL_0607
AMOL: AMOL_1804
AANA: AANAER_1824
DTO: TOL2_C06430(qhpA)
DHG: EYB58_07975(peaA)
DVR: Dvar_52670
MIN: Minf_1997 Minf_2000
MKC: kam1_317 kam1_320
MFH: MFUM_0347 MFUM_0350(aauA)
MCAO: IT6_04770 IT6_04785
BPIT: BPIT_26730
TALB: FTW19_10610(peaA)
BACO: OXB_3506
BKN: WDJ61_07915(peaA)
NMK: CHR53_12040(peaA)
BBAD: K7T73_07500(peaA)
PTL: AOT13_15015
ANX: ACH33_09155
ATHE: K3F53_09935(peaA)
CPRE: Csp1_26450(mauA)
RER: RER_53630
REY: O5Y_25460
REB: XU06_25810
RQI: C1M55_27045
RPY: Y013_17135
RBY: CEJ39_03535
RAV: AAT18_05020
RRZ: CS378_20525
RHQ: IM25_07085
RHOD: AOT96_07005
RRT: 4535765_00133(mauA)
RCR: NCTC10994_01857(mauA)
RTM: G4H71_05040
RGO: KYT97_16925
RHOP: D8W71_22610
RHOO: RA302_26060
RHOU: KZJ41_05565
RHOW: A4U64_19525
RHOS: QWW67_22505
RHOQ: QWW98_22515
RHOY: QNA09_03095
RHOH: CPI83_03470
RHOX: RBB84_21715
RSF: OED52_00610
RHZG: BO226_18235
RHBH: A0W34_25680
RHPD: U9J23_00600
RHS: A3Q41_02066(mauA)
REQ: REQ_44990(mauA)
DIZ: CT688_08630
MIO: AOA12_17540
MOJ: D7D94_07795
 ยป show all
Reference
1  [PMID:6246962]
  Authors
de Beer R, Duine JA, Frank J, Large PJ.
  Title
The prosthetic group of methylamine dehydrogenase from Pseudomonas AM1: evidence for a quinone structure.
  Journal
Biochim Biophys Acta 622:370-4 (1980)
DOI:10.1016/0005-2795(80)90050-1
Reference
2  [PMID:4388687]
  Authors
Eady RR, Large PJ.
  Title
Purification and properties of an amine dehydrogenase from Pseudomonas AM1 and its role in growth on methylamine.
  Journal
Biochem J 106:245-55 (1968)
DOI:10.1042/bj1060245
Reference
3  [PMID:5124384]
  Authors
Eady RR, Large PJ.
  Title
Microbial oxidation of amines. Spectral and kinetic properties of the primary amine dehydrogenase of Pseudomonas AM1.
  Journal
Biochem J 123:757-71 (1971)
DOI:10.1042/bj1230757
Reference
4  [PMID:18512962]
  Authors
Cavalieri C, Biermann N, Vlasie MD, Einsle O, Merli A, Ferrari D, Rossi GL, Ubbink M
  Title
Structural comparison of crystal and solution states of the 138 kDa complex of methylamine dehydrogenase and amicyanin from Paracoccus versutus.
  Journal
Biochemistry 47:6560-70 (2008)
DOI:10.1021/bi7023749
Reference
5  [PMID:20873742]
  Authors
Meschi F, Wiertz F, Klauss L, Cavalieri C, Blok A, Ludwig B, Heering HA, Merli A, Rossi GL, Ubbink M
  Title
Amicyanin transfers electrons from methylamine dehydrogenase to cytochrome c-551i via a ping-pong mechanism, not a ternary complex.
  Journal
J Am Chem Soc 132:14537-45 (2010)
DOI:10.1021/ja105498m
Other DBs
ExplorEnz - The Enzyme Database: 1.4.9.1
IUBMB Enzyme Nomenclature: 1.4.9.1
ExPASy - ENZYME nomenclature database: 1.4.9.1
UM-BBD (Biocatalysis/Biodegradation Database): 1.4.9.1
BRENDA, the Enzyme Database: 1.4.9.1
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