Oxidoreductases;
Acting on the CH-NH group of donors;
With an iron-sulfur protein as acceptor
BRITE hierarchy

N,N-dimethylglycine/sarcosine:ferredoxin oxidoreductase (demethylating)

(1) N,N-dimethylglycine + 2 oxidized ferredoxin + H2O = sarcosine + formaldehyde + 2 reduced ferredoxin + 2 H+ [RN:R13028];
(2) sarcosine + 2 oxidized ferredoxin + H2O = glycine + formaldehyde + 2 reduced ferredoxin + 2 H+ [RN:R13029]

R13028 R13029
Reaction

N,N-dimethylglycine [CPD:C01026];
oxidized ferredoxin [CPD:C00139];
H2O [CPD:C00001];
sarcosine [CPD:C00213]

This bacterial enzyme is involved in degradation of glycine betaine. The enzyme contains non-covalently bound FAD and NAD(P) cofactors, and catalyses the demethylation of both N,N-dimethylglycine and sarcosine, releasing formaldehyde and forming glycine as the final product. The enzyme can utilize both NAD+ and NADP+, but the catalytic efficiency with NAD+ is ~5-fold higher. The native electron acceptor of the enzyme is a membrane-bound clostridial-type ferredoxin, which transfers the electrons to an electron-transfer flavoprotein (ETF).

EC 1.5.7.3 created 2022

1  [PMID:17951379]

2  [PMID:32631860]