KEGG ENZYME: 1.5.8.3

ENZYME: 1.5.8.3

Entry

EC 1.5.8.3                  Enzyme

Name

sarcosine dehydrogenase;
sarcosine N-demethylase;
monomethylglycine dehydrogenase;
sarcosine:(acceptor) oxidoreductase (demethylating);
sarcosine:electron-transfer flavoprotein oxidoreductase (demethylating)

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With a flavin or flavoprotein as acceptor

Sysname

sarcosine, 5,6,7,8-tetrahydrofolate:electron-transferflavoprotein oxidoreductase (demethylating,5,10-methylenetetrahydrofolate-forming)

Reaction(IUBMB)

sarcosine + 5,6,7,8-tetrahydrofolate + oxidized [electron-transfer flavoprotein] = glycine + 5,10-methylenetetrahydrofolate + reduced [electron-transfer flavoprotein] [RN:R00611]

Reaction(KEGG)

R00611

Substrate

sarcosine [CPD:C00213];
5,6,7,8-tetrahydrofolate [CPD:C00101];
oxidized [electron-transfer flavoprotein] [CPD:C04253]

Product

glycine [CPD:C00037];
5,10-methylenetetrahydrofolate [CPD:C00143];
reduced electron-transfer flavoprotein [CPD:C04570]

Comment

A flavoprotein (FMN) found in eukaryotes. In the absence of tetrahydrofolate the enzyme produces formaldehyde. cf. EC 1.5.3.1, sarcosine oxidase (formaldehyde-forming), and EC 1.5.3.24, sarcosine oxidase (5,10-methylenetetrahydrofolate-forming).

History

EC 1.5.8.3 created 1972 as EC 1.5.99.1, transferred 2012 to EC 1.5.8.3, modified 2022

Pathway

ec00260

Glycine, serine and threonine metabolism

ec01100

Metabolic pathways

Orthology

K00314

sarcosine dehydrogenase

Genes

HSA:	1757(SARDH)

PTR:	464828(SARDH)

PPS:	100986136(SARDH)

GGO:	101126679(SARDH)

PON:	100172073(SARDH)

PPYG:

129044476(SARDH)

NLE:	100581547(SARDH)

HMH:	116475832(SARDH)

SSYN:

129478713(SARDH)

MCC:	713289(SARDH)

MCF:	102139165(SARDH)

MTHB:

126936903

MNI:	105471877(SARDH)

CSAB:

103239675(SARDH)

CATY:

105578092 105580522(SARDH)

PANU:

101015658(SARDH)

TGE:	112608502(SARDH) 112632442

MLEU:

105547817(SARDH)

RRO:	104669713(SARDH)

RBB:	108513254(SARDH)

TFN:	117099201(SARDH)

PTEH:

111524002(SARDH)

CANG:

105503971(SARDH)

CJC:	100396240(SARDH)

SBQ:	101038416(SARDH)

CIMI:

108292304(SARDH)

ANAN:

105712262(SARDH)

CSYR:

103273376(SARDH)

MMUR:

105880753(SARDH)

LCAT:

123645984(SARDH)

PCOQ:

105826695(SARDH)

OGA:	100950320(SARDH)

MMU:	192166(Sardh)

MCAL:

110310298(Sardh)

MPAH:

110318656(Sardh)

RNO:	114123(Sardh)

MCOC:

116089881(Sardh)

ANU:	117704184(Sardh)

MUN:	110557507(Sardh)

CGE:	100761121(Sardh)

MAUA:

101837481(Sardh)

PROB:

127222657(Sardh)

PLEU:

114684225(Sardh)

MORG:

121462827(Sardh)

MFOT:

126509210

AAMP:

119818433(Sardh)

NGI:	103742819(Sardh)

HGL:	101717296(Sardh)

CPOC:

100718504(Sardh)

CCAN:

109695047(Sardh)

DORD:

105995659(Sardh)

DSP:	122108903(Sardh)

PLOP:

125353290(Sardh)

NCAR:

124964225

MMMA:

107159138(Sardh)

OPI:	101531608(SARDH)

TUP:	102468288(SARDH)

GVR:	103581999 103607776

CFA:	491277(SARDH)

CLUD:

112677395(SARDH)

VVP:	112935890(SARDH)

VLG:	121472927(SARDH)

NPO:	129505342(SARDH)

AML:	100477821(SARDH)

UMR:	103679941(SARDH)

UAH:	113266576(SARDH)

UAR:	123775645(SARDH)

ELK:

111146213

LLV:

125083188

MPUF:

101689810(SARDH)

MNP:	132024271(SARDH)

MLK:	131812505(SARDH)

NVS:	122917099(SARDH)

ORO:	101363163(SARDH)

EJU:	114200995(SARDH)

ZCA:	113933946(SARDH)

MLX:	118017541(SARDH)

NSU:	110581413(SARDH)

LWW:	102727859(SARDH)

FCA:	101082702(SARDH)

PYU:	121021238(SARDH)

PBG:	122475224(SARDH)

PVIV:

125150065(SARDH)

LRUF:

124522436

PTG:	102959524(SARDH)

PPAD:

109258271(SARDH)

PUC:

125923035

AJU:

106988257

HHV:	120247321(SARDH)

BTA:	614603(SARDH)

BOM:	102286478(SARDH)

BIU:	109566616(SARDH)

BBUB:

102407897(SARDH)

BBIS:

104983331(SARDH)

CHX:	102176539(SARDH)

OAS:	101110407(SARDH)

BTAX:

128056075(SARDH)

ODA:	120852212(SARDH)

CCAD:

122442336(SARDH)

MBEZ:

129539422(SARDH)

SSC:	100154338(SARDH)

CFR:	102524074(SARDH)

CBAI:

105068574(SARDH)

CDK:	105102809(SARDH)

VPC:	102539312(SARDH)

BACU:

103001806(SARDH)

BMUS:

118896710(SARDH)

LVE:	103082579(SARDH)

OOR:	101269445(SARDH)

DLE:	111177031(SARDH)

PCAD:

114487445

PSIU:

116755110(SARDH)

NASI:

112411329(SARDH)

ECB:	100069193(SARDH)

EPZ:	103564041(SARDH)

EAI:	106845348(SARDH)

MYB:	102263827(SARDH)

MYD:	102763367(SARDH)

MMYO:

118666898(SARDH)

MDT:	132212529(SARDH)

PKL:	118708904(SARDH)

EFUS:

103296146(SARDH)

MNA:	107524617(SARDH)

DRO:	112306574(SARDH)

SHON:

118980108(SARDH)

AJM:	119036072(SARDH)

PDIC:

114502600(SARDH)

PHAS:

123823382(SARDH)

MMF:	118629383(SARDH)

PPAM:

129079162(SARDH)

HAI:	109381141(SARDH)

RFQ:	117032350(SARDH)

PALE:

102882489(SARDH)

PGIG:

120618873(SARDH)

PVP:	105308211(SARDH)

RAY:	107506266(SARDH)

MJV:	108388196(SARDH)

TOD:	119231222(SARDH)

SARA:

101550003(SARDH)

SETR:

126009328(SARDH)

LAV:

100677707

TMU:

101344177

ETF:	101656739(SARDH)

DNM:	101446999(SARDH)

MDO:	100018850(SARDH)

GAS:	123237208(SARDH)

SHR:	100925921(SARDH)

AFZ:

127550957

PCW:	110205373(SARDH)

TVP:	118841799(SARDH)

OAA:	100073946(SARDH)

GGA:	417146(SARDH)

PCOC:

116234304(SARDH)

MGP:	100544931(SARDH)

CJO:	107321866(SARDH)

TPAI:

128073307(SARDH)

LMUT:

125702507(SARDH)

NMEL:

110406941(SARDH)

APLA:

101793569(SARDH)

ACYG:

106044083(SARDH)

CATA:

118252264(SARDH)

AFUL:

116496886(SARDH)

TGU:	100221431(SARDH)

LSR:	110477625(SARDH)

SCAN:

103819130(SARDH)

PMOA:

120512467(SARDH)

OTC:	121337015(SARDH)

PRUF:

121362436(SARDH)

GFR:	102042792(SARDH)

FAB:	101807275(SARDH)

OMA:	130260255(SARDH)

PHI:	102100623(SARDH)

PMAJ:

107211981(SARDH)

CCAE:

111937046(SARDH)

CCW:	104688488(SARDH)

ETL:	114059122(SARDH)

ZAB:	102072207(SARDH)

ZLE:	135454925(SARDH)

ACHL:

103807317(SARDH)

SVG:	106851371(SARDH)

MMEA:

130576689(SARDH)

HRT:	120761614(SARDH)

SATI:

136369787(SARDH)

FPG:	101910621(SARDH)

FCH:	102055614(SARDH)

CCRI:

104161252(SARDH)

NNT:	104401845(SARDH)

SHAB:

115617139(SARDH)

ACUN:

113486534(SARDH)

TALA:

104358507(SARDH)

ACHC:

115335020(SARDH)

HLE:	104834021(SARDH)

AGEN:

126052419

GCL:

127023702

CSTI:

104559665(SARDH)

LDI:	104353408(SARDH)

DPUB:

104296481(SARDH)

AVIT:

104275305(SARDH)

BRHI:

104496130(SARDH)

EGZ:	104129358(SARDH)

NNI:	104023287(SARDH)

PCRI:

104028748(SARDH)

PADL:

103915344(SARDH)

AFOR:

103905337(SARDH)

CLV:	102089708(SARDH)

MUI:	104537222(SARDH)

PGUU:

104462250(SARDH)

EHS:	104510227(SARDH)

CMAC:

104484056(SARDH)

CUCA:

104066940(SARDH)

TEO:	104375627(SARDH)

BREG:

104632937(SARDH)

OHA:	104329475(SARDH)

ACAR:

104524269(SARDH)

CPEA:

104389905(SARDH)

CVF:	104292890(SARDH)

RTD:	128917707(SARDH)

AAM:	106484478(SARDH)

AROW:

112964334(SARDH)

NPD:	112955996(SARDH)

TGT:	104568109(SARDH)

DNE:	112979933(SARDH)

SCAM:

104138293(SARDH)

ASN:

102387705

AMJ:	102565496(SARDH)

CPOO:

109313993(SARDH)

GGN:	109293014(SARDH)

PSS:	102455800(SARDH)

CMY:	102936719(SARDH)

CCAY:

125623620(SARDH)

DCC:	119844196(SARDH)

CPIC:

101938284(SARDH)

TST:	117867214(SARDH)

CABI:

116817467(SARDH)

MRV:	120386569(SARDH)

ACS:	100567227 103281982(sardh)

ASAO:

132763715(SARDH)

PVT:	110089290(SARDH)

SUND:

121937135(SARDH)

PBI:

103065615

PMUR:

107291024(SARDH)

CTIG:

120310204(SARDH)

TSR:	106552855(SARDH)

PGUT:

117671326(SARDH)

APRI:

131186144(SARDH)

PTEX:

113448476(SARDH)

NSS:	113426289(SARDH)

VKO:	123035146(SARDH)

PMUA:

114588913(SARDH)

PRAF:

128406238(SARDH)

ZVI:	118083953(SARDH)

HCG:	128338781(SARDH)

GJA:	107106570(SARDH)

STOW:

125441578(SARDH)

EMC:	129342011(SARDH)

XLA:	108698171(sardh.L)

XTR:	100492087(sardh)

NPR:

108793117

RTEM:

120913855(SARDH)

BBUF:

120978222(SARDH)

BGAR:

122946102(SARDH)

MUO:	115473063(SARDH)

GSH:	117368407(SARDH)

DRE:	394103(sardh)

SRX:	107713264 107744257

SANH:

107685122(sardh)

SGH:	107595553(sardh)

CCAR:

109052549 109064274

CAUA:

113109650 113109661

CGIB:

127966273

PTET:

122352742(sardh)

LROH:

127171925(sardh)

OMC:	131548321(sardh)

PPRM:

120482668(sardh)

RKG:	130073503(sardh)

MAMB:

125257695(sardh)

CIDE:

127521118

CERY:

137028883(sardh)

MASI:

127432966

TROS:

130546400(sardh)

TDW:	130411579(sardh)

MANU:

129447250(sardh)

IPU:

108276635

IFU:	128620169 128620698(sardh)

PHYP:

113544041(sardh)

SMEO:

124393246(sardh)

TFD:	113653074(sardh)

TVC:	132863207(sardh)

TRN:	134332200(sardh)

AMEX:

103038491(sardh)

CMAO:

118824921(sardh)

EEE:	113576323(sardh)

CHAR:

105906040(sardh)

TRU:	101066393(sardh)

TFS:	130517643(sardh)

TNG:	GSTEN00024728G001

LCO:	104923713(sardh)

NCC:	104968189(sardh)

TBEN:

117476396(sardh)

CGOB:

115016604(sardh)

PGEO:

117456399(sardh)

GACU:

117534085(sardh)

EMAC:

134868707(sardh)

ELY:	117270080(sardh)

EFO:	125905775(sardh)

PLEP:

121959519(sardh)

SLUC:

116034954(sardh)

ECRA:

117959051(sardh)

ESP:	116704714(sardh)

PFLV:

114571014(sardh)

GAT:	120832222(sardh)

PPUG:

119226499(sardh)

AFB:	129102463(sardh)

CLUM:

117740414(sardh)

PSWI:

130205130(sardh)

MSAM:

119914706(sardh)

SCHU:

122865194(sardh)

CUD:	121525654(sardh)

ALAT:

119030407(sardh)

MZE:	101464347(sardh)

ONL:	100708425(sardh)

OAU:	116335193(sardh)

OLA:	101171504(sardh)

OML:	112162906(sardh)

CSAI:

133455326(sardh)

XMA:	102218956(sardh)

XCO:	114149434(sardh)

XHE:	116724090(sardh)

PRET:

103473627(sardh)

PFOR:

103147877(sardh)

PLAI:

106948297(sardh)

PMEI:

106921877(sardh)

GAF:	122847257(sardh)

PPRL:

129366447(sardh)

CVG:	107088710(sardh)

CTUL:

119782613(sardh)

GMU:	124872392(sardh)

NFU:	107372413(sardh)

KMR:	108232690(sardh)

ALIM:

106514558(sardh)

NWH:	119424550(sardh)

AOCE:

111567010(sardh)

MCEP:

124996692(sardh)

CSEM:

103389066(sardh)

POV:	109626083(sardh)

SSEN:

122758351(sardh)

HHIP:

117771594(sardh)

HSP:	118125404(sardh)

PPLT:

128424548(sardh)

SMAU:

118284742(sardh)

LCF:

108879253

SDU:	111238764(sardh)

SLAL:

111666073(sardh)

XGL:	120806360(sardh)

HCQ:	109517069(sardh)

SSCV:

125980042

SBIA:

133490562(sardh)

PEE:	133414082(sardh)

PTAO:

133489894(sardh)

BPEC:

110174148(sardh)

MALB:

109963453(sardh)

BSPL:

114866771(sardh)

SJO:	128380397(sardh)

SASA:

106561570(sardh)

STRU:

115159137(sardh)

OTW:	112235511(sardh)

OMY:	110523941(sardh)

OGO:	124048581(sardh)

ONE:	115127526(sardh)

OKI:	109868567(sardh)

OKE:	118387955(sardh)

SALP:

111961266(sardh)

SNH:	120026194(sardh)

CCLU:

121582864(sardh)

ELS:	105014778(sardh)

SFM:	108932522(sardh)

PKI:	111854167(sardh)

AANG:

118212851(sardh)

LOC:	102683068(sardh)

PSPA:

121303520(sardh) 121307070

ARUT:

117396956 117422502

PSEX:

120535140(sardh)

LCM:	102358838(SARDH)

CMK:	103183089(sardh)

HOC:	132825979(sardh)

LERI:

129712112(sardh)

PMRN:

116953236(SARDH)

LRJ:	133354673(SARDH)

BFO:

118421387

BBEL:

109465509

CIN:	100179924 100182727 100184925

SCLV:

120326566 120326837 120326875 120343979

SPU:

576973

LPIC:

129278088

APLC:

110984820

ARUN:

117303284

AJC:

117102826

SKO:	100374616(Sardh)

DME:	Dmel_CG6385(Sardh)

DER:

6546660

DSE:

6609685

DSI:	Dsimw501_GD25443(Dsim_GD25443)

DYA:	Dyak_GE13964

DAN:

6496069

DSR:

110191126

DPO:

4805367

DPE:

6592810

DMN:	108159573 117193886

DWI:

6652181

DGR:

6562281

DMO:	Dmoj_GI23785

DAZ:

108610357

DNV:

115562207

DHE:

111600959

DVI:

6628364

CCAT:

101460422

BOD:

106618894

BDR:

105228945

RZE:

108359402

AOQ:

129245314

TDA:

119680841

MDE:

101899451

SCAC:

106091682

LCQ:

111688870

LSQ:	119613729 119614622

GFS:

119641567

ECOE:

129951912

HIS:

119655277

AGA:

1269983

ACOZ:

120951228

AARA:

120907545

AMER:

121591818

ASTE:

118511380

AFUN:

125767575

AMOU:

128304153

AALI:

118465372

AAG:	5565677 5572760

AALB:

109403674 109407988

ASUA:

134210618

CQU:	CpipJ_CPIJ004591 CpipJ_CPIJ016929

CPII:

120420098

CNS:	116345252 116348710 116349036

BCOO:

119067948 119079361

BIM:

100748086

BBIF:

117208124

BVK:

117235543

BVAN:

117154114

BTER:

100643113

BAFF:

126916392

BPYO:

122565774

BPAS:

132905386

FVI:

122534812

CCAL:

108629225

OBB:

114879267

OLG:

117610733

MGEN:

117227684

NMEA:

116432029

CGIG:

122398593

SOC:

105195588

MPHA:

105835145

AEC:	105144562 105144729

ACEP:

105618338

PBAR:

105431690

VEM:

105569751

HST:

105184399

DQU:

106746182

CFO:

105253367

FEX:

115234381

LHU:	105673907(Sardh)

PGC:

109857207

OBO:

105288106

PCF:

106788912

PFUC:

122517706

VPS:

122638164

VCRB:

124426914

VVE:

124946579

NVI:

103316656

CSOL:

105364420

TPRE:

106655629

LHT:

122508053

LBD:

127280105

MDL:

103575471

CGLO:

123272457

FAS:

105267456

DAM:

107038954

AGIF:

122855053

CINS:

118064118

VCAN:

122410613

CCIN:

107273333

DSM:

124407662

NPT:

124214228

NFB:

124177765

NLO:

107217484

NVG:

124300370

AROA:

105684623

TCA:

656849

DPA:

109533033

SOY:

115885282

AGRG:

126736428

ATD:

109597723

CSET:

123310519

AGB:

108903272

LDC:

111502476

DVV:

126884307

NVL:

108568210

APLN:

108738448

PPYR:

116169980 116169982 116172170

OTU:

111425006

BMOR:

101743034

BMAN:

114247185

MSEX:

115453464

DPL:	KGM_204504

BANY:

112050165

MJU:

123864905

NIQ:

126779451

VCD:

124541659

MCIX:

123660816

PMAC:

106710382

PPOT:

106108150

PXU:

106117377

PRAP:

110993772

PBX:

123707243

PNAP:

125055796

ZCE:

119830144

CCRC:

123703688

LSIN:

126965660

AAGE:

121729279

HAW:

110382187

HZE:

124643796

TNL:

113492560

SLIU:

111357600

OFU:

114360011

ATRA:

106129444

GMW:

113522951

PXY:

105381895

PGW:

126381603

LGLY:

125241262

CFEL:

113365503 113365504 113386018

CCRN:

123296798

CLEC:

106666323

HHAL:

106690642(Sardh)

MQU:

128992710

PHU:	Phum_PHUM213030

FOC:

113207710

TPAL:

117641156

ZNE:	110831180 110831181

CSEC:

111870731 111870733

SGRE:

126299609

BROR:

134536721

IEL:

124169506

FCD:

110856220

DPX:	DAPPUDRAFT_308482

DMK:

116930444

DPZ:

124338461

PVM:	113821151 113822901

PJA:	122255467 122265981

PCHN:

125026237 125034535

PMOO:

119579938 119589287

HAME:

121870600 121870602

PCLA:

123764040

CQD:

128701620

PTRU:

123508387 123508458

ESN:	126981425 126986885

MNZ:	135198650 135203034 135203035 135203036 135220766

HAZT:

108675385

EAF:

111713614

LSM:

121119332

PPOI:

119103280

ISC:	8034007 8036170

DSV:

119437074

RSAN:

119394083

RMP:

119161133

VDE:

111248602

VJA:

111265115

CSCU:

111628631

CVS:	136986961(Sardh)

PTEP:

107449896

ABRU:

129981674

UDV:

129223402

SDM:

118185941

LPOL:

106471624

LJP:

135480924

LAK:

106167453

LLON:

135500943

NVE:

5503178

EPA:

110254849

ATEN:

116292581

ADF:

107344630

AMIL:

114976066

PDAM:

113668163

SPIS:

111329248

DGT:

114530955

HMG:

100197405

HSY:

130662453

RES:

135693684

TAD:	TRIADDRAFT_53512

AAF:	AURANDRAFT_22613(SDH1)

SPAR:

SPRG_03481

EHX:	EMIHUDRAFT_97687

» show all

Reference

1 [PMID:13716069]

Authors

HOSKINS DD, MACKENZIE CG.

Title

Solubilization and electron transfer flavoprtein requirement of mitochondrial sarcosine dehydrogenase and dimethylglycine dehydrogenase.

Journal

J Biol Chem 236:177-83 (1961)

Reference

2 [PMID:13895406]

Authors

FRISELL WR, MACKENZIE CG.

Title

Separation and purification of sarcosine dehydrogenase and dimethylglycine dehydrogenase.

Journal

J Biol Chem 237:94-8 (1962)

Reference

3 [PMID:6163778]

Authors

Wittwer AJ, Wagner C

Title

Identification of the folate-binding proteins of rat liver mitochondria as dimethylglycine dehydrogenase and sarcosine dehydrogenase. Flavoprotein nature and enzymatic properties of the purified proteins.

Journal

J Biol Chem 256:4109-15 (1981)

Reference

4 [PMID:6180732]

Authors

Steenkamp DJ, Husain M

Title

The effect of tetrahydrofolate on the reduction of electron transfer flavoprotein by sarcosine and dimethylglycine dehydrogenases.

Journal

Biochem J 203:707-15 (1982)
DOI:10.1042/bj2030707

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